Reconstruction of diploid higher-order human 3D genome interactions from noisy Pore-C data using Dip3D [0.03%]
利用Pore-C噪声数据重构二倍体高阶人类3D基因组相互作用的Dip3D方法
Ying Chen,Zhuo-Bin Lin,Shao-Kai Wang et al.
Ying Chen et al.
Differential high-order chromatin interactions between homologous chromosomes affect many biological processes. Traditional chromatin conformation capture genome analysis methods mainly identify two-way interactions and cannot provide compr...
CTCF-mediated 3D chromatin sets up the gene expression program in the male germline [0.03%]
由CTCF介导的3D染色体建立了雄性生殖系的基因表达程序
Yuka Kitamura,Kazuki Takahashi,So Maezawa et al.
Yuka Kitamura et al.
Spermatogenesis is a unidirectional differentiation process that generates haploid sperm, but how the gene expression program that directs this process is established is largely unknown. Here we determine the high-resolution three-dimension...
Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR) [0.03%]
关于涉及真核生物双基因表达报告系统的实验的最小报告要求、设计和解释指南(MINDR)
Gary Loughran,Dmitry E Andreev,Ilya M Terenin et al.
Gary Loughran et al.
Dual reporters encoding two distinct proteins within the same mRNA have had a crucial role in identifying and characterizing unconventional mechanisms of eukaryotic translation. These mechanisms include initiation via internal ribosomal ent...
A chromatin mesh model for compaction of chromatin by PRC1 in condensates [0.03%]
凝聚物中PRC1介导的染色质压缩的染色质网模型
Nicole J Francis
Nicole J Francis
ZBTB16/PLZF regulates juvenile spermatogonial stem cell development through an extensive transcription factor poising network [0.03%]
ZBTB16/PLZF通过广泛的转录因子启动网络调控幼年精原干细胞的发育
Chongil Yi,Yuka Kitamura,So Maezawa et al.
Chongil Yi et al.
Spermatogonial stem cells balance self-renewal with differentiation and spermatogenesis to ensure continuous sperm production. Here, we identify roles for the transcription factor zinc finger and BTB domain-containing protein 16 (ZBTB16; al...
Human polymerase θ helicase positions DNA microhomologies for double-strand break repair [0.03%]
人类聚合酶θ解旋酶定位用于双链断裂修复的DNA微同源区
Christopher J Zerio,Yonghong Bai,Brian A Sosa-Alvarado et al.
Christopher J Zerio et al.
DNA double-strand breaks occur daily in all human cells and must be repaired with high fidelity to minimize genomic instability. Deficiencies in high-fidelity DNA repair by homologous recombination lead to dependence on DNA polymerase θ, w...
Structures of a natural circularly permuted group II intron reveal mechanisms of branching and backsplicing [0.03%]
天然的环状移位II型内含子的结构揭示了其分支和回切机制
Xiaobin Ling,Yuqi Yao,Jinbiao Ma
Xiaobin Ling
Circularly permuted (CP) group II introns, identified in various bacteria phyla, swap domains D5 and D6 near the 5' end and have reversed splice sites (SSs), leading to backsplicing and circular RNA formation. In this study, we present mult...
A PAR6-aPKC-LGL structure reveals how LGL antagonizes aPKC [0.03%]
一个PAR6-aPKC-LGL结构揭示了LGL如何拮抗aPKC的作用
Daniel St Johnston
Daniel St Johnston
Xiaoying Chen,Licheng Yuan,Han Wen et al.
Xiaoying Chen et al.
Broad-range thermal receptor 1 (BRTNaC1), activated by heat at low extracellular pH, was recently identified in myriapods. Although the overexpression of BRTNaC1 leads to robust heat-activated current with a cation selectivity profile, the ...
Local structural flexibility drives oligomorphism in computationally designed protein assemblies [0.03%]
局部结构柔性驱动了计算设计的蛋白质组装中的寡形态现象
Alena Khmelinskaia,Neville P Bethel,Farzad Fatehi et al.
Alena Khmelinskaia et al.
Many naturally occurring protein assemblies have dynamic structures that allow them to perform specialized functions. Although computational methods for designing novel self-assembling proteins have advanced substantially over the past deca...