Elena A Zehr,Shufeng Sun,Stephanie L Sarbanes et al.
Elena A Zehr et al.
Microtubules scaffold cells, supporting signaling and cargo transport. They assemble from GTP-tubulin, which hydrolyzes to GDP-tubulin during polymerization. GTP-microtubule lattices are stable; GDP lattices depolymerize rapidly. In vitro, ...
Do-Hwan Ahn,Claudia Alleva,Tom Reichenbach et al.
Do-Hwan Ahn et al.
In mammals, glucose transporters (GLUTs) mediate organism-wide sugar distribution, yet the molecular basis of substrate specificity remains unclear. The bacterial xylose transporter XylE serves as a model for GLUTs. However, although xylose...
Gregg Duester
Gregg Duester
Kotaro Tsukada,Yuka Nakazawa,Tomoo Ogi
Kotaro Tsukada
MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy [0.03%]
Stephen P Burr,Kathryn Auckland,Angelos Glynos et al.
Stephen P Burr et al.
Mitochondria contain their own genome, mitochondrial DNA (mtDNA), which is under strict control by the cell nucleus. mtDNA occurs in many copies per cell and mutations often only affect a proportion of them, giving rise to heteroplasmy. mtD...
Arijit Dutta,Jae-Hoon Ji,Shahrez Syed et al.
Arijit Dutta et al.
Senataxin (SETX), an RNA-DNA helicase, accumulates at transcription pause sites through the tumor suppressor BRCA1. Here, we provide mechanistic insight into how SETX-BRCA1 resolves transcription-associated R-loops to prevent deleterious ou...
Yu Liu,Minghui Song,Liqi Wan et al.
Yu Liu et al.
Biomolecular condensates regulate essential biological processes relevant to health and disease. However, the mechanisms driving pathogenic condensate formation and their therapeutic targeting have not been fully elucidated. In amyotrophic ...
Feizuo Wang,Ruochen Guo,Senfeng Zhang et al.
Feizuo Wang et al.
IscB, a compact Cas9 ancestor from the obligate mobile element guided activity system, has attracted growing interest as a programmable genome editor because of its small size and therapeutic delivery potential. Despite its promise, structu...