Functional RNA splitting drove the evolutionary emergence of type V CRISPR-Cas systems from transposons [0.03%]
功能RNA切割驱动了V型CRISPR-Cas系统从转座子中进化出现
Shuai Jin,Zixu Zhu,Yunjia Li et al.
Shuai Jin et al.
Transposon-encoded TnpB nucleases gave rise to type V CRISPR-Cas12 effectors through multiple independent domestication events. These systems use different RNA molecules as guides for DNA targeting: transposon-derived right-end RNAs (reRNAs...
Single-cell nascent transcription reveals sparse genome usage and plasticity [0.03%]
单细胞新生转录揭示了稀疏的基因组利用和可塑性
Shaoqian Ma,Yantao Hong,Junhan Chen et al.
Shaoqian Ma et al.
Understanding cell diversification from a common genome in metazoans requires single-cell transcriptional analysis. We introduce single-cell full-length EU-labeled nascent RNA sequencing (scFLUENT-seq), a single-cell nascent RNA sequencing ...
Multiscale proteomic modeling reveals protein networks driving Alzheimer's disease pathogenesis [0.03%]
多尺度蛋白质组学建模揭示了驱动阿尔茨海默病发病机制的蛋白质网络
Erming Wang,Kaiwen Yu,Jiqing Cao et al.
Erming Wang et al.
The molecular mechanisms underlying the pathogenesis of Alzheimer's disease (AD), the most common form of dementia, remain poorly understood. Proteomics offers a crucial approach to elucidating AD pathogenesis, as alterations in protein exp...
Safe immunosuppression-resistant pan-cancer immunotherapeutics by velcro-like density-dependent targeting of tumor-associated carbohydrate antigens [0.03%]
类似胶带的糖基抗原密度依赖性靶向实现安全有效的广谱免疫治疗
Raymond W Zhou,Paresh Kumar Purohit,Jai Hyun Kim et al.
Raymond W Zhou et al.
Bispecific antibodies and chimeric antigen receptor T cells are some of the most potent cancer immunotherapeutics in clinical use, yet most cancers remain poorly targetable. High-affinity antibodies required to maximize killing detect low a...
Intrinsic heterogeneity of primary cilia revealed through spatial proteomics [0.03%]
空间蛋白质组学揭示原发纤毛的内在异质性
Jan N Hansen,Huangqingbo Sun,Konstantin Kahnert et al.
Jan N Hansen et al.
Primary cilia are critical organelles found on most human cells. Their dysfunction is linked to hereditary ciliopathies with a wide phenotypic spectrum. Despite their significance, the specific roles of cilia in different cell types remain ...
Astrocytes functionally integrate multiple synapses via specialized leaflet domains [0.03%]
astrocyte通过专门的叶片结构功能上整合多个突触
Lucas Benoit,Ines Hristovska,Nicolas Liaudet et al.
Lucas Benoit et al.
Astrocyte Ca2+ dynamics control synaptic circuits and behavior, yet the underlying biology remains poorly understood. By combining volumetric high-resolution electron microscopy and two-photon Ca2+ imaging, we characterize astrocyte leaflet...
Maria Ciofani,Aviv Madar,Carolina Galan et al.
Maria Ciofani et al.
Published Erratum
Cell. 2025 Sep 23:S0092-8674(25)01083-9. DOI:10.1016/j.cell.2025.09.015 2025
Time-resolved fluorescent proteins expand fluorescent microscopy in temporal and spectral domains [0.03%]
时间解析荧光蛋白可拓展荧光显微镜的时间和光谱维度
Zizhu Tan,Chia-Heng Hsiung,Jiahui Feng et al.
Zizhu Tan et al.
Fluorescence microscopy has been widely applied in the life sciences. While intensity as a steady-state signal is widely used, the time-resolved (tr) signal using fluorescence lifetime remains underexplored. Herein, we present a family of t...
Maria Akopyan,Matthew Genchev,Ellie E Armstrong et al.
Maria Akopyan et al.
Characterizing genetic variation in natural populations is vital to evolutionary biology; however, many non-model species lack genomic resources. Here, we demonstrate that reference bias significantly affects population genomic analyses by ...
James H Leebens-Mack
James H Leebens-Mack
In this issue of Cell, Huang and colleagues reveal how ancient hybridization between ancestors of tomato and a related wild species, Solanum etuberosum, enabled the origin of tuber formation and the diversification of potato species. Their ...