An anti-CRISPR targets the sgRNA to block Cas9 and guides the design of enhanced genome editors [0.03%]
一种抗CRISPR蛋白通过靶向sgRNA来抑制Cas9活性并用于优化基因编辑系统
Ling Yu,Mingyu Yin,Yuwei Zhu et al.
Ling Yu et al.
Bacteriophages have evolved anti-CRISPR (Acr) proteins to combat the adaptive immunity provided by bacterial CRISPR-Cas systems. Here, we report the cryo-electron microscopy structure of an anti-Cas9 protein AcrIIA27 bound to SpyCas9-sgRNA ...
Interplay between cohesin and RNA polymerase II in regulating chromatin interactions and gene transcription [0.03%]
调控染色质相互作用和基因转录的粘连素与RNA聚合酶II之间的互作关系
Minji Kim,Ping Wang,Patricia A Clow et al.
Minji Kim et al.
Cohesin is required for chromatin loop formation. However, its precise role in regulating gene transcription remains largely debated. Here we investigated the relationship between cohesin and RNA polymerase II (RNAPII) using single-molecule...
Redox-driven ADAR1 activation promotes Okazaki fragment maturation and DNA replication integrity [0.03%]
氧化还原驱动的ADAR1活化促进Okazaki片段成熟和DNA复制完整性
Bin Chen,Guangchao Sun,Jake A Kloeber et al.
Bin Chen et al.
Okazaki fragment maturation requires efficient removal of RNA primers to form a continuous lagging strand, yet how mismatched primers introduced by error-prone primase are corrected remains unresolved. Here, we show that physiological level...
Cell-cycle-dependent repression of histone gene transcription by histone H4 [0.03%]
组蛋白H4在细胞周期依赖性地抑制组蛋白基因转录
Kami Ahmad,Matt Wooten,Brittany N Takushi et al.
Kami Ahmad et al.
In all eukaryotes, DNA replication is coupled to histone synthesis to coordinate chromatin packaging of the genome. Canonical histone genes coalesce in the nucleus into the histone locus body (HLB), where gene transcription and 3' mRNA proc...
Tommy O&#x;Haren,Leila E Rieder
Tommy O&#x;Haren
Evolution of promoter-proximal pausing enabled a new layer of transcription control [0.03%]
promoters-proximal暂停的进化使转录控制的新层次成为可能
Alexandra G Chivu,Brent A Basso,Abderhman Abuhashem et al.
Alexandra G Chivu et al.
Promoter-proximal pausing of RNA polymerase (Pol) II is a key regulatory step during transcription. Despite the central role of pausing in gene regulation, we do not understand the evolutionary processes that led to the emergence of Pol II ...
Ifigenia Tsitsa,Anja Conev,Alessia David et al.
Ifigenia Tsitsa et al.