Improving the efficiency of high-fidelity Cas9 by enhancing PAM-distal interactions [0.03%]
通过增强PAM远端相互作用来提高高保真Cas9的效率
Rong Zheng,Zhike Lu,Rongwei Wei et al.
Rong Zheng et al.
Engineering CRISPR enzymes for high fidelity often impairs cleavage activity. Meanwhile, a mechanistic understanding of why high-fidelity mutations reduce Cas9's cleavage activity remains unclear, presenting a challenge in balancing nucleas...
Toshifumi Inada
Toshifumi Inada
Ribosome-associated quality control (RQC) safeguards translation by detecting and resolving collided ribosomes and triaging their nascent chains. This Review outlines mechanisms, crosstalk and disease implications of RQC cascades and presen...
Dimitris Typas
Dimitris Typas
Checking in on proteostasis [0.03%]
检查蛋白质稳态
Eri Sakata,Ramanujan S Hegde,Yan G Zhao et al.
Eri Sakata et al.
Kirandeep K Deol,Cynthia A Harris,Sydney J Tomlinson et al.
Kirandeep K Deol et al.
Ferroptosis, a regulated form of cell death driven by excessive lipid peroxidation, has emerged as a promising therapeutic target in cancer. Ferroptosis suppressor protein 1 (FSP1) is a critical regulator of ferroptosis resistance, yet the ...