Structural basis of AtCas9 recognition of PAM mutants in underwound DNA topology [0.03%]
AtCas9在DNA超螺旋结构中识别PAM突变的机制研究
Min Duan,Bing Meng,Lei Zhou et al.
Min Duan et al.
The CRISPR-Cas9 system locates targets through guide RNA pairing and recognition of a protospacer-adjacent motif (PAM). Although PAM specificity is sequence-determined, DNA topology can relax PAM requirements and enable near-PAMless cleavag...
Catalytic activation of human Argonaute 2 requires RNA duplex deformation [0.03%]
人类Argonaute 2活化需要RNA双链变形催化
Sucharita Sarkar,Luca F R Gebert,Ian J MacRae
Sucharita Sarkar
Small interfering RNAs (siRNAs) are an expanding class of RNA therapeutics, with seven drugs approved by the US Food and Drug Administration and many more in development. Rational design, however, has been limited by incomplete understandin...
Structural basis of opioid receptor activation by PCP and ketamine [0.03%]
PCP和氯胺酮通过作用于阿片受体激活的结构基础研究
Qianru Jiang,Jianming Han,Eve J Fine et al.
Qianru Jiang et al.
Ketamine offers rapid relief for treatment-resistant depression and severe pain in the clinic, providing immediate benefits that traditional medications often fail to deliver. While its antagonistic action at the N-methyl-D-aspartate recept...
Dimitris Typas
Dimitris Typas
Sara Osman
Sara Osman
Structural insights into OSTα/β-mediated transport of bile acids and steroid conjugates [0.03%]
OSTα/β介导的胆汁酸和类固醇结合物转运的结构见解
Xicheng Sun,Taikun Tian,Minying Low et al.
Xicheng Sun et al.
Mammalian organic solute transporter α/β (OSTα/β) is crucial for the enterohepatic circulation of bile acids and the homeostasis of steroid conjugates, mediating their movement across membranes as an obligate heterodimer. Here we presen...
Sammy H S Chan,Julian O Streit,Tomasz Włodarski et al.
Sammy H S Chan et al.
The ribosome biases the conformations sampled by nascent polypeptide chains along folding pathways toward biologically active states. A hallmark of the cotranslational folding (coTF) of many proteins constitutes highly stable folding interm...
A single-vesicle fluorescence microscopy platform to quantify phospholipid scrambling [0.03%]
一种单脂质体荧光显微镜平台用于量化磷脂打乱现象
Sarina Veit,Grace I Dearden,Kartikeya M Menon et al.
Sarina Veit et al.
Scramblases are physiologically important proteins that translocate phospholipids bidirectionally across cell membranes. For example, scrambling facilitated by dimers of the voltage-dependent anion channel 1 (VDAC1) enables endoplasmic reti...
Semirandom DNA adducts regulate a filamentous defense-associated reverse transcriptase [0.03%]
半随机DNA加合物调节丝状抗病相关逆转录酶
Nolan Neville,Nicole V Johnson,Edwin E Escobar et al.
Nolan Neville et al.
Retrons and several defense-associated reverse transcriptases (DRTs) synthesize non-genomic DNA for bacteriophage immunity. In some instances, this non-genomic DNA is of undefined, semirandom sequence. How undefined DNA sequences impart ant...