Long-term reversal of Duchenne muscular dystrophy via circular arRNA-guided exon skipping in monkeys and humans [0.03%]
通过圆形单链arRNA引导的外显子跳跃逆转猴子和人类杜氏肌营养不良症
Wenting Guo,Huixian Tang,Zongyi Yi et al.
Wenting Guo et al.
Duchenne muscular dystrophy (DMD) is a fatal neuromuscular disorder caused by mutations in the DMD gene, leading to progressive muscle degeneration, loss of mobility, and premature death. Here, we applied leverage endogenous adenosine deami...
Persistent and transient senescent cells contribute to brain-barrier development [0.03%]
持久性和瞬时性衰老细胞有助于脑屏障发育
L Ashley Watson,Zoe Adelsheim,Mackenzie J Carter et al.
L Ashley Watson et al.
Establishment of the blood-brain barrier (BBB) and blood-cerebrospinal fluid (CSF) barrier requires precise coordination between diverse cell types to protect and nourish the brain. Here, we identify developmentally programmed p21+ senescen...
Prion-based protein self-assembly tunes mutagenesis to enable rapid adaptation [0.03%]
基于朊病毒的蛋白质自组装调节突变以实现快速适应
Alexandria Van Elgort,Christopher M Jakobson,Yiwen R Chen et al.
Alexandria Van Elgort et al.
Mutations supply the raw material for evolution, yet because most are neutral or deleterious, elevated mutation rates are typically transient. Nonetheless, modeling predicts that a mechanism for heritable but reversible "mutagenesis switche...
mRNA 3' UTRs chaperone intrinsically disordered regions to control protein activity [0.03%]
mRNA 3'UTR通过分子伴侣作用调控内在无序区以控制蛋白质活性
Yang Luo,Yaofeng Zhong,Sudipto Basu et al.
Yang Luo et al.
More than 2,700 human mRNA 3' UTRs have hundreds of highly conserved nucleotides, but their biological roles are unclear. These mRNAs encode proteins strongly enriched for long intrinsically disordered regions (IDRs) with hydrophobic amino ...
Sicong Wang,Kouta Niizuma,Daniel Dan Liu et al.
Sicong Wang et al.
Organ shortage remains a major challenge in transplantation medicine. Interspecies blastocyst complementation offers a promising strategy to generate human organs in livestock. However, efficient xenogeneic donor cell engraftment remains ch...
William S DeWitt,Ashni A Vora,Tatsuya Araki et al.
William S DeWitt et al.
Darwinian evolution of immunoglobulin genes within germinal centers (GCs) underlies the progressive increase in antibody affinity following antigen exposure. Whereas the cellular mechanics of how competition between B cells increases affini...
Deep-sea megafauna co-opts microbial energy metabolism genes to withstand ultra-long starvation [0.03%]
深海大型动物劫持微生物能量代谢基因以应对极度饥饿事件
Jianbo Yuan,Xiaojun Zhang,Shihao Li et al.
Jianbo Yuan et al.
The deep-sea supergiant isopod is renowned for surviving over 5 years without food, which is a crucial adaptive trait for megafauna inhabiting extreme environments. Here, morphological, physiological, and genomic comparisons of deep-sea iso...
Single-cell mapping of regulatory DNA-protein interactions [0.03%]
单细胞染色质相互作用分析技术mapping of Regulatory DNA-Protein Interactions (MRI)
Wei-Yu Chi,Sang-Ho Yoon,Evrim Goksel et al.
Wei-Yu Chi et al.
Gene expression is controlled by transcription factors (TFs), whose genome binding is shaped by chromatin accessibility and histone modifications, yet mapping these interactions, particularly those with weak affinity or a transient nature, ...
Anita Donlic,Troy J Comi,Sofia A Quinodoz et al.
Anita Donlic et al.
Biomolecular condensates compartmentalize the interior of cells to organize complex functions, yet linking molecular interactions within condensates to their mesoscale organization remains a major challenge. To bridge this gap, we developed...
Tej Pandya,Maria Zagorulya,Michelle M Leung et al.
Tej Pandya et al.
Predicting lung cancer risk would enhance prevention trials. Although the Canakinumab Anti-inflammatory Thrombosis Outcome Study (CANTOS) trial demonstrated reduced lung cancer incidence with interleukin (IL)-1β inhibition, the high number...