MitoPerturb-Seq identifies gene-specific single-cell responses to mitochondrial DNA depletion and heteroplasmy [0.03%]
MitoPerturb-Seq识别线粒体DNA消耗和杂合性引起的基因特异性单细胞反应
Stephen P Burr,Kathryn Auckland,Angelos Glynos et al.
Stephen P Burr et al.
Mitochondria contain their own genome, mitochondrial DNA (mtDNA), which is under strict control by the cell nucleus. mtDNA occurs in many copies per cell and mutations often only affect a proportion of them, giving rise to heteroplasmy. mtD...
Resolution of R-loops and transcription-replication conflicts by SETX-BRCA1-BARD1 complex [0.03%]
SETX-BRCA1-BARD1复合体解决R环和转录-复制冲突的作用
Arijit Dutta,Jae-Hoon Ji,Shahrez Syed et al.
Arijit Dutta et al.
Senataxin (SETX), an RNA-DNA helicase, accumulates at transcription pause sites through the tumor suppressor BRCA1. Here, we provide mechanistic insight into how SETX-BRCA1 resolves transcription-associated R-loops to prevent deleterious ou...
Condensate protein aggregation in ALS/FTD is regulated by GGGGCC-repeat RNA scaffolds [0.03%]
含G四联体重复序列的RNA支架调控ALS/FTD中的胞浆蛋白聚集体形成
Yu Liu,Minghui Song,Liqi Wan et al.
Yu Liu et al.
Biomolecular condensates regulate essential biological processes relevant to health and disease. However, the mechanisms driving pathogenic condensate formation and their therapeutic targeting have not been fully elucidated. In amyotrophic ...
Feizuo Wang,Ruochen Guo,Senfeng Zhang et al.
Feizuo Wang et al.
IscB, a compact Cas9 ancestor from the obligate mobile element guided activity system, has attracted growing interest as a programmable genome editor because of its small size and therapeutic delivery potential. Despite its promise, structu...
Improving the efficiency of high-fidelity Cas9 by enhancing PAM-distal interactions [0.03%]
通过增强PAM远端相互作用来提高高保真Cas9的效率
Rong Zheng,Zhike Lu,Rongwei Wei et al.
Rong Zheng et al.
Engineering CRISPR enzymes for high fidelity often impairs cleavage activity. Meanwhile, a mechanistic understanding of why high-fidelity mutations reduce Cas9's cleavage activity remains unclear, presenting a challenge in balancing nucleas...
Toshifumi Inada
Toshifumi Inada
Ribosome-associated quality control (RQC) safeguards translation by detecting and resolving collided ribosomes and triaging their nascent chains. This Review outlines mechanisms, crosstalk and disease implications of RQC cascades and presen...
Dimitris Typas
Dimitris Typas
Checking in on proteostasis [0.03%]
检查蛋白质稳态
Eri Sakata,Ramanujan S Hegde,Yan G Zhao et al.
Eri Sakata et al.