Composite transposons with bivalent histone marks function as RNA-dependent enhancers in cell fate regulation [0.03%]
复合组蛋白修饰的转座子通过RNA依赖的方式发挥作用并调节细胞命运
Ziqiang Zhou,Shicong Zhu,Yaqiang Hong et al.
Ziqiang Zhou et al.
Discrete genomic units can recombine into composite transposons that transcribe and transpose as single units, but their regulation and function are not fully understood. We report that composite transposons harbor bivalent histone marks, w...
Ju-Chan Park,Heesoo Uhm,Yong-Woo Kim et al.
Ju-Chan Park et al.
The prime editing (PE) system consists of a Cas9 nickase fused to a reverse transcriptase, which introduces precise edits into the target genomic region guided by a PE guide RNA. However, PE efficiency is limited by mismatch repair. To over...
Iterative recombinase technologies for efficient and precise genome engineering across kilobase to megabase scales [0.03%]
迭代重组酶技术实现从千碱基到百万碱基数尺度的高效精准基因组编辑
Chao Sun,Hongchao Li,Yijing Liu et al.
Chao Sun et al.
Genome editing technologies face challenges in achieving precise, large-scale DNA manipulations in higher organisms, including inefficiency, limited editing scales and types, and the retention of undesired sequences such as recombination si...
Ruonan Di,Qianqian Du,Yuhua Xie et al.
Ruonan Di et al.
The transition from water to land required animals to evolve specialized paw skin to support body weight and enable locomotion. We identify an evolutionarily emerged mechanism in skin epithelial cells that adapts to this mechanical demand. ...
Single-cell multiregion epigenomic rewiring in Alzheimer's disease progression and cognitive resilience [0.03%]
阿尔茨海默病进展和认知韧性的单细胞多区域表观基因组重构
Zunpeng Liu,Shanshan Zhang,Benjamin T James et al.
Zunpeng Liu et al.
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive cognitive decline, yet its epigenetic underpinnings remain elusive. Here, we generate and integrate single-cell epigenomic and transcriptomic profiles of ...
Design of soluble Notch agonists that drive T cell development and boost immunity [0.03%]
可溶性Notch激动剂的设计用于驱动T细胞发育和增强免疫反应
Rubul Mout,Ran Jing,Mayuri Tanaka-Yano et al.
Rubul Mout et al.
The rational design of receptor agonists to control cell signaling is an emerging strategy for developing disease therapeutics. Creating a soluble cytokine-like agonist for the Notch receptor, which regulates cell fate in embryonic and adul...
Ancient hybridization underlies tuberization and radiation of the potato lineage [0.03%]
古老的杂交作用构成了 Potato 这条进化线中块茎形成及多样化的基础
Zhiyang Zhang,Pingxian Zhang,Yiyuan Ding et al.
Zhiyang Zhang et al.
Interspecific hybridization may trigger species radiation by creating allele combinations and traits. Cultivated potato and its 107 wild relatives from the Petota lineage all share the distinctive trait of underground tubers, but the underl...
Quantifying the varying harvest of fermentation products from the human gut microbiota [0.03%]
量化人类肠道微生物群发酵产物的收获量
Markus Arnoldini,Richa Sharma,Claudia Moresi et al.
Markus Arnoldini et al.
Fermentation products released by the gut microbiota provide energy and regulatory functions to the host. Yet, little is known about the magnitude of this metabolic flux and its quantitative dependence on diet and microbiome composition. He...
DNA mutagenesis driven by transcription factor competition with mismatch repair [0.03%]
转录因子与错配修复的竞争驱动的DNA突变发生率提升现象
Wei Zhu,Yuning Zhang,Harshit Sahay et al.
Wei Zhu et al.
Despite the remarkable fidelity of eukaryotic DNA replication, nucleotide misincorporation errors occur in every replication cycle, generating mutations that drive genetic diseases and genome evolution. Here, we show that transcription fact...
Kiwa is a membrane-embedded defense supercomplex activated at phage attachment sites [0.03%]
黏附在噬菌体附着点的膜整合防御超复合物Kiwa
Zhiying Zhang,Thomas C Todeschini,Yi Wu et al.
Zhiying Zhang et al.
Bacteria and archaea deploy diverse antiviral defense systems, many of which remain mechanistically uncharacterized. Here, we characterize Kiwa, a widespread two-component system composed of the transmembrane sensor KwaA and the DNA-binding...