Biotechnology letters. 2025 Apr 16;47(3):42. doi: 10.1007/s10529-025-03576-6 Q32.12025

Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2

SARS-CoV-2 N蛋白靶向重组杆状病毒的构建及其免疫原性分析翻译改进

Bohan Yu¹, Xiaoli Mo¹, Li Sui¹, Yujia Fang¹, Xudong Tang^{1 2}, Ping Qian^{3 4}

作者单位 +展开

作者单位

¹ Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China.

² Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang, 212100, China.

³ Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212100, China. qianping@just.edu.cn.

⁴ Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Scientific Research Center, Chinese Academy of Agricultural Sciences, Zhenjiang, 212100, China. qianping@just.edu.cn.

DOI: 10.1007/s10529-025-03576-6 PMID: 40237823

摘要中英对照阅读

Objectives: This study aimed to construct recombinant baculoviruses capable of expressing the nucleocapsid N protein of SARS-CoV-2 and to assess the effects of co-administration with sodium butyrate on its expression and immunogenicity. Results: The recombinant BmNPV expressed green fluorescent protein in BmN cells and N protein in mammalian... ...点击完成人机验证后继续浏览

目标： 本研究旨在构建能够表达SARS-CoV-2核衣壳N蛋白的重组杆状病毒，并评估与丁酸钠共给药对其表达和免疫原性的影响。

结果： 重组BmNPV在BmN细胞中表达了绿色荧光蛋白，在哺乳动物细胞中表达了N蛋白。添加丁酸钠显著增强了HEK293T细胞中N蛋白的表达。将重组BmNPV肌肉注射到小鼠体内后，分别在第7天、第21天和第35天检测到了针对N蛋白的特异性抗体。与丁酸钠（每公斤体重1000毫克）共给药显著增强了重组病毒的免疫原性。

结论： 表达SARS-CoV-2 N蛋白的重组BmNPV成功地在小鼠中诱导了抗-N免疫原性，为开发针对SARS-CoV-2的新亚单位疫苗提供了坚实的基础。

关键词： 杆状病毒；COVID-19 N蛋白；免疫原性蛋白质表达；丁酸钠。

翻译效果不满意？用Ai改进或寻求AI助手帮助，对摘要进行重点提炼

相关内容

期刊名：Biotechnology letters

缩写：BIOTECHNOL LETT

ISSN：0141-5492

e-ISSN：1573-6776

IF/分区：2.1/Q3

文章目录更多期刊信息

全文链接

官方链接

PMC全文

引文链接

复制

已复制！

格式：

Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2

SARS-CoV-2 N蛋白靶向重组杆状病毒的构建及其免疫原性分析翻译改进

Construction and immunogenicity of SARS-CoV-2 virus-like particle expressed by recombinant baculovirus BacMam

由重组杆状病毒BacMam表达的SARS-CoV-2病毒样颗粒的构建及免疫原性研究

Detection of SARS-CoV-2 N protein allelic variants by rapid high-throughput CLEIA antigen assay

基于CLEIA抗原快速高通量检测SARS-CoV-2 N蛋白等位基因变异

Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus

含SARS-CoV-2 N蛋白质粒的A549细胞转染效率优化研究

[Preparation of different fragments of SARS-CoV-2 N protein and its application in fluorescence chromatography]

SARS-CoV-2核蛋白不同片段的表达及其在荧光色谱中的应用

The SARS-CoV-2 N Protein Is a Good Component in a Vaccine

SARS-CoV-2核蛋白可作为疫苗成分

Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies

SARS-CoV-2核蛋白的多种作用机制及其与RNA、宿主蛋白质和恢复期抗体特异互作的作用形式功能多样性

SARS-CoV-2 N Protein Targets TRIM25-Mediated RIG-I Activation to Suppress Innate Immunity

新型冠状病毒N蛋白通过靶向TRIM25介导的RIG-I活化来抑制天然免疫反应

SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron

SARS-CoV-2 N蛋白介导的细胞间核酸扩散，在奥密克戎变异株中该特征减弱

SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation

SARS-CoV-2核蛋白通过促进NLRP3炎症小体激活诱导细胞过度炎症反应

Construction and immunogenicity analysis of a recombinant baculovirus targeting the N protein of SARS-CoV-2

SARS-CoV-2 N蛋白靶向重组杆状病毒的构建及其免疫原性分析 翻译改进

Construction and immunogenicity of SARS-CoV-2 virus-like particle expressed by recombinant baculovirus BacMam

由重组杆状病毒BacMam表达的SARS-CoV-2病毒样颗粒的构建及免疫原性研究

Detection of SARS-CoV-2 N protein allelic variants by rapid high-throughput CLEIA antigen assay

基于CLEIA抗原快速高通量检测SARS-CoV-2 N蛋白等位基因变异

Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus

含SARS-CoV-2 N蛋白质粒的A549细胞转染效率优化研究

[Preparation of different fragments of SARS-CoV-2 N protein and its application in fluorescence chromatography]

SARS-CoV-2核蛋白不同片段的表达及其在荧光色谱中的应用

The SARS-CoV-2 N Protein Is a Good Component in a Vaccine

SARS-CoV-2核蛋白可作为疫苗成分

Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies

SARS-CoV-2核蛋白的多种作用机制及其与RNA、宿主蛋白质和恢复期抗体特异互作的作用形式功能多样性

SARS-CoV-2 N Protein Targets TRIM25-Mediated RIG-I Activation to Suppress Innate Immunity

新型冠状病毒N蛋白通过靶向TRIM25介导的RIG-I活化来抑制天然免疫反应

SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron

SARS-CoV-2 N蛋白介导的细胞间核酸扩散，在奥密克戎变异株中该特征减弱

SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation

SARS-CoV-2核蛋白通过促进NLRP3炎症小体激活诱导细胞过度炎症反应

SARS-CoV-2 N蛋白靶向重组杆状病毒的构建及其免疫原性分析翻译改进