Doklady. Biochemistry and biophysics. 2025 Apr 11. doi: 10.1134/S1607672924601136 Q40.82025

Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus

含SARS-CoV-2 N蛋白质粒的A549细胞转染效率优化研究翻译改进

Yu V Khramtsov¹, T N Lupanova¹, A A Rosenkranz^{1 2}, G P Georgiev¹, A S Sobolev^{3 4}

作者单位 +展开

作者单位

¹ Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.

² Moscow State University, Moscow, Russia.

³ Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia. alsobolev@yandex.ru.

⁴ Moscow State University, Moscow, Russia. alsobolev@yandex.ru.

DOI: 10.1134/S1607672924601136 PMID: 40216710

摘要中英对照阅读

To test new antiviral drugs aimed at degrading the nucleocapsid protein (N-protein) of the SARS-CoV-2 virus, it is desirable to have cells expressing the N-protein, for which it is necessary to find conditions for the maximum achievable efficiency of cell transfection with a plasmid encoding this protein. For transfection, polyplexes were used consisting of a plasmid encoding the N-protein fused with the mRuby3 fluorescent protein and polyethyleneimine (PE... ...

请您完成人机验证后继续浏览

为了测试旨在降解SARS-CoV-2病毒核衣壳蛋白（N蛋白）的新型抗病毒药物，需要有表达该蛋白的细胞。为此，有必要找到使细胞转染含有编码此蛋白质粒效率达到最大的条件。在转染过程中使用了由编码与mRuby3荧光蛋白融合的N蛋白的质粒和聚乙烯亚胺（PEI）-聚乙二醇（PEG）-TAT肽嵌段共聚物组成的聚复合物。研究了人肺腺癌A549细胞转染效率随PEG/PEI比例及N/P比值（PEI中的氮与DNA中磷酸根的比例）变化的依赖关系。通过流式细胞术测定的转染效率、N/P比率以及尺寸在40-54 nm范围内的聚复合物所占比例之间存在显著正相关性。获得的数据可以作为建立一种瞬时表达SARS-CoV-2病毒核蛋白的动物肺细胞模型的基础。

关键词：
SARS-CoV-2；动态光散射；流式细胞术；核衣壳蛋白；聚乙烯亚胺；聚复合物。

翻译效果不满意？用Ai改进或寻求AI助手帮助，对摘要进行重点提炼

相关内容

期刊名：Doklady biochemistry and biophysics

缩写：DOKL BIOCHEM BIOPHYS

ISSN：1607-6729

e-ISSN：1608-3091

IF/分区：0.8/Q4

文章目录更多期刊信息

全文链接

官方链接

PMC全文

引文链接

复制

已复制！

格式：

Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus

含SARS-CoV-2 N蛋白质粒的A549细胞转染效率优化研究翻译改进

Detection of SARS-CoV-2 N protein allelic variants by rapid high-throughput CLEIA antigen assay

基于CLEIA抗原快速高通量检测SARS-CoV-2 N蛋白等位基因变异

[Preparation of different fragments of SARS-CoV-2 N protein and its application in fluorescence chromatography]

SARS-CoV-2核蛋白不同片段的表达及其在荧光色谱中的应用

The SARS-CoV-2 N Protein Is a Good Component in a Vaccine

SARS-CoV-2核蛋白可作为疫苗成分

Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies

SARS-CoV-2核蛋白的多种作用机制及其与RNA、宿主蛋白质和恢复期抗体特异互作的作用形式功能多样性

SARS-CoV-2 N Protein Targets TRIM25-Mediated RIG-I Activation to Suppress Innate Immunity

新型冠状病毒N蛋白通过靶向TRIM25介导的RIG-I活化来抑制天然免疫反应

SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron

SARS-CoV-2 N蛋白介导的细胞间核酸扩散，在奥密克戎变异株中该特征减弱

SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation

SARS-CoV-2核蛋白通过促进NLRP3炎症小体激活诱导细胞过度炎症反应

The method utilized to purify the SARS-CoV-2 N protein can affect its molecular properties

用于纯化SARS-CoV-2 N蛋白的方法会影响其分子特性

Optimization of A549 Cell Transfection Efficiency with a Plasmid Encoding the N-Protein of the SARS-CoV-2 Virus

含SARS-CoV-2 N蛋白质粒的A549细胞转染效率优化研究 翻译改进

Detection of SARS-CoV-2 N protein allelic variants by rapid high-throughput CLEIA antigen assay

基于CLEIA抗原快速高通量检测SARS-CoV-2 N蛋白等位基因变异

[Preparation of different fragments of SARS-CoV-2 N protein and its application in fluorescence chromatography]

SARS-CoV-2核蛋白不同片段的表达及其在荧光色谱中的应用

The SARS-CoV-2 N Protein Is a Good Component in a Vaccine

SARS-CoV-2核蛋白可作为疫苗成分

Multiple Roles of SARS-CoV-2 N Protein Facilitated by Proteoform-Specific Interactions with RNA, Host Proteins, and Convalescent Antibodies

SARS-CoV-2核蛋白的多种作用机制及其与RNA、宿主蛋白质和恢复期抗体特异互作的作用形式功能多样性

SARS-CoV-2 N Protein Targets TRIM25-Mediated RIG-I Activation to Suppress Innate Immunity

新型冠状病毒N蛋白通过靶向TRIM25介导的RIG-I活化来抑制天然免疫反应

SARS-CoV-2 N protein mediates intercellular nucleic acid dispersion, a feature reduced in Omicron

SARS-CoV-2 N蛋白介导的细胞间核酸扩散，在奥密克戎变异株中该特征减弱

SARS-CoV-2 N protein promotes NLRP3 inflammasome activation to induce hyperinflammation

SARS-CoV-2核蛋白通过促进NLRP3炎症小体激活诱导细胞过度炎症反应

The method utilized to purify the SARS-CoV-2 N protein can affect its molecular properties

用于纯化SARS-CoV-2 N蛋白的方法会影响其分子特性

含SARS-CoV-2 N蛋白质粒的A549细胞转染效率优化研究翻译改进