Droplet based microfluidic and miniaturization at high-throughput level to screening heterologous secondary metabolites in microbes: A critical review [0.03%]
Enver Felix Loayza Mora
Enver Felix Loayza Mora
Droplet based microfluidics and automation are important technology to support bio-process and screening during the last years. Main objective of this review is to describe the current technology in monitoring secreting microbes at scale do...
Arabitol production: influence of acid and salt stress on glycerol fermentation by Komagataella pastoris, Scheffersomyces stipitis, and Pichia kluyveri in a batch system [0.03%]
Ana Karen Calderón-Ordaz,Ana Alejandra Vargas-Tah,Agustín Jaime Castro-Montoya et al.
Ana Karen Calderón-Ordaz et al.
Yeasts of the genus Pichia include osmophilic species that metabolize glycerol and produce arabitol. This research aimed to evaluate the effects of acid and salt stress on enhancing the yield of arabitol in a batch system. It also innovates...
Glycerol-supplemented medium promotes transition of Cupriavidus necator from heterotrophic to lithoautotrophic growth [0.03%]
甘油补充培养基促进土壤细菌由异养向自养的转变
I Castro Gonzalez,N Gorret,L Lauterbach et al.
I Castro Gonzalez et al.
H2-driven biotransformations in the chemolithoautotrophic bacterium, Cupriavidus necator, are gaining attention to obtain valuable industrial molecules. However, cultivation variability and long lag phases have been reported in C. necator c...
Characterization and substrate specificity study of the novel (R)-amine transaminase MagAT [0.03%]
新型(R)胺转氨酶MagAT的鉴定及其底物特异性研究
Xiaole Yang,Xia Tian,Hai Zhu et al.
Xiaole Yang et al.
Chiral amines, as essential chiral building blocks in drug synthesis, present a considerable challenge in biomanufacturing due to the requirement for highly efficient stereoselective synthesis. In this study, we successfully cloned and hete...
Enhancing leucine aminopeptidase activity in Aspergillus oryzae LL1 through multi-round UV irradiation mutagenesis with potential application in debittering [0.03%]
通过多轮紫外线诱变强化稻曲霉LL1亮氨肽酶活性及其脱苦应用潜力研究
Chiu-Yen Wen,Bo-Chou Chen,Huan-Yu Lin
Chiu-Yen Wen
To enhance the applicability of Aspergillus oryzae LL1 in the food industry, the strain underwent ultraviolet irradiation through random mutagenesis to enhance the enzyme activity of extracellular leucine aminopeptidase (LAP). A. oryzae LL1...
Aysin Cavdar,Emine Karakus,Cigdem Bilen
Aysin Cavdar
Polyphenol oxidase (PPO) enzymes perform enzymatic browning reactions by hydroxylation of monophenols to o-diphenols and oxidation of o-diphenols to o-quinones called phenolic substances in foods. Medlar fruit (Mespilus germanica L.) was us...
Coloring target and off-target effects of genetically modified nucleases by blue & white colony assays [0.03%]
利用蓝白斑筛选检测基因编辑的靶标和脱靶效应
Xumeng Chen,Li Xiao,Qian Wang et al.
Xumeng Chen et al.
More sensitive evaluation of the off-target effects of gene editing nucleases is crucial for human gene therapy. Here we report chromogenic assays designed for sensitive evaluation of gene editing activities using CRISPR/Cas9 test system. B...
Enhancing production of nicotinamide mononucleotide by metabolic engineering and flow cytometry screening of Escherichia coli [0.03%]
代谢工程结合流式细胞术筛选提高大肠杆菌 NMN 产量
Lu Yang,Yanwei Wei,Suzhen Yang et al.
Lu Yang et al.
β-Nicotinamide mononucleotide (NMN) is a nucleotide that serves as a vital source of cellular energy and a direct precursor of nicotinamide adenine dinucleotide (NAD+), a crucial cofactor in human metabolism. Given its significant value in...
The Bacillus subtilis expansin BsEXLX1 enhances cellobiohydrolase I activity on sugar beet pulp as shown by QCM-D [0.03%]
枯草芽孢杆菌膨胀素BsEXLX1通过QCM-D证明可增强糖用甜菜浆中纤维素酶CBH I的活性
Nathan Cannac,Anne S Meyer
Nathan Cannac
Microbial expansins are proteins that are thought to mediate loosening of plant cell wall networks by non-catalytic disruption of non-covalent bonds between polysaccharides, particularly between cellulose chains. As a result, the expansins ...
Ultrasonic high efficiency preparation of arctigenin from the fruits of Arctium lappa L using Aspergillus niger with deep eutectic solvent assisted [0.03%]
黑曲霉结合深共晶溶剂辅助高强度超声波提取牛膝子中阿魏酸茋酚酮类成分阿魏茋酮的研究
Yubin Ren,Shuang Jin,Weili Liu et al.
Yubin Ren et al.
Arctigenin, a high-value lignan with broad bioactivities, suffers from low yield in conventional extraction. This study developed a green and efficient process integrating natural deep eutectic solvent (NADES)-pretreated Aspergillus niger f...