Long-read metagenomics reveals stable resistome and microbiome in treated Italian slaughterhouse wastewater: a preliminary study [0.03%]
基于长读段的意大利屠宰废水处理前后的元基因组研究：初步报告
Stefano Pallotti,Maria Elena Nigro,Elisa Albini et al.
Stefano Pallotti et al.
Antimicrobial resistance (AMR) poses a major threat to global health, and food production environments are increasingly recognized as potential reservoirs and dissemination points for resistant bacteria and antimicrobial resistance genes (A...
CRISPR-Cas12a-based fluorescent and visual assays for universal detection and clade discrimination of mpox virus [0.03%]
基于CRISPR-Cas12a的荧光和可视检测法广泛用于猴痘病毒的鉴定和分支区分
Yingqing Mao,Xinyu Fei,Xijing Yang et al.
Yingqing Mao et al.
The global mpox outbreak has highlighted critical gaps in diagnostic capabilities, particularly the need for methods that can distinguish between the high-fatality Clade I and more transmissible Clade II of the mpox virus (MPXV). Current PC...
Cytotoxicity mechanism of heterologous expression of phospholipase D in Escherichia coli [0.03%]
异源表达的磷脂酶D对大肠杆菌毒性的机理研究
Shaofeng Chen,Rongxin Wu,Xiaoyun Guo et al.
Shaofeng Chen et al.
Phospholipase D (PLD) has multiple functions in cells and various industrial applications, including the production of high-purity and rare phospholipids, making it a highly valuable enzyme. However, the strong cytotoxicity of PLD and limit...
Mycoplasma hyopneumoniae modulates ciliary function and epithelial integrity in air-liquid interface porcine respiratory epithelial cells (ALI-PRECs) [0.03%]
猪肺炎支原体通过空气-液体界面培养的猪呼吸上皮细胞模型调控纤毛功能和上皮屏障功能
Ana F Castillo-Espinoza,Rahul K Nelli,Juan C Mora-Díaz et al.
Ana F Castillo-Espinoza et al.
Mycoplasma hyopneumoniae (Mhp), the causative agent of enzootic pneumonia, disrupts mucociliary clearance by adhering to porcine ciliated airway cells, contributing to chronic respiratory disease. Traditional submerged cell cultures lack ai...
Mycobacterium tuberculosis does not inhibit NLRP1 and pyrin inflammasomes in human macrophages [0.03%]
结核分枝杆菌不抑制人巨噬细胞中的NLRP1和pyrin炎性小体
Akshaya Ganesh,Shivangi Rastogi,Volker Briken
Akshaya Ganesh
Mycobacterium tuberculosis (Mtb) has been known to evade host innate immunity by manipulating macrophage function. Interleukin-1β (IL-1β) is a cytokine secreted by macrophages as a consequence of inflammasome activation. Mtb can inhibit a...
ClpC1-dependent iron homeostasis underlies mycobacterial defense against oxygen-driven Fenton reaction during reactivation [0.03%]
缺氧诱发的Fenton反应引起结核病复发的新机制
Mingzhe Chi,Yuqing Zheng,Wenkai Zheng et al.
Mingzhe Chi et al.
The caseinolytic protease (Clp) system, which is highly conserved and essential for protein homeostasis in mycobacteria, comprises the ClpP1P2 proteolytic core and the essential ATPases ClpC1 and ClpX. While ClpX has a defined role in DNA r...
Evaluation of BD Phoenix CPO Detect and NG-Test CARBA 5 for the detection and classification of carbapenemase-producing Enterobacterales using whole-genome sequencing as the reference standard [0.03%]
全基因组测序作为参考标准，评估BD凤凰CPO检测和NG-测试CARBA 5检测和分类碳青霉烯类药物耐药肠杆菌目细菌的能力
Nisha Aji,Namitha Thomas,Andrés Pérez-López et al.
Nisha Aji et al.
Rapid detection and accurate classification of carbapenemase-producing Enterobacterales (CPE) are essential for antimicrobial stewardship and infection prevention. We evaluated the performance of the BD Phoenix CPO Detect (BD-CPO) assay and...
Development of a novel, urine-based high-risk human papillomavirus polymerase chain reaction test to predict cervical intraepithelial neoplasia abnormalities associated with cervical cancer [0.03%]
一种新型尿液高危人乳头瘤病毒聚合酶链式反应检测方法预测宫颈上皮内瘤变及宫颈癌筛查研究进展
Vasu Saini,Zhengyang Guo,Jinwen Yu et al.
Vasu Saini et al.
This study evaluated and optimized a novel urine-based workflow using high-risk human papillomavirus (hrHPV) polymerase chain reaction (PCR) test to detect 14 hrHPV genotypes linked to cervical intraepithelial neoplasia (CIN) abnormalities ...
A study on cadmium immobilization by bacterial consortia: Achromobacter insuavis SL8 and Enterobacter cancerogenus SL12 [0.03%]
一株芽孢杆菌和一株肠杆菌共培养对镉的固定作用研究
Yu Tao,Shengying Ji,Chi Zhou et al.
Yu Tao et al.
Microbial bioremediation represents one of the most environmentally sustainable strategies for mitigating cadmium (Cd) pollution. This study employed an integrated approach combining spectroscopy, metabolomics, and proteomics to elucidate t...
Genomic analysis of antimicrobial resistance and virulence among gram-negative bloodstream isolates from Lebanon [0.03%]
来自黎巴嫩革兰氏阴性血流分离株的抗微生物药物耐药性和毒力的基因组分析
Carni Boujanian,Charbel Al Khoury,Mira El Chaar et al.
Carni Boujanian et al.
The increasing prevalence of multidrug-resistant (MDR) gram-negative bacteria has reduced therapeutic options for bloodstream infections (BSIs) and complicates clinical management. In Lebanon, limited genomic surveillance has restricted ins...