Novel Fas-TNFR chimeras that prevent Fas ligand-mediated kill and signal synergistically to enhance CAR T cell efficacy [0.03%]
新颖的Fas-TNFR嵌合体可防止Fas配体介导的杀伤并协同信号以增强CAR-T细胞的疗效
Callum McKenzie,Mohamed El-Kholy,Farhaan Parekh et al.
Callum McKenzie et al.
The hostile tumor microenvironment limits the efficacy of adoptive cell therapies. Activation of the Fas death receptor initiates apoptosis and disrupting these receptors could be key to increasing CAR T cell efficacy. We screened a library...
The miR-148/152 family contributes to angiogenesis of human pluripotent stem cell- derived endothelial cells by inhibiting MEOX2 [0.03%]
miR-148/152家族通过抑制MEOX2促进人多能干细胞源性内皮细胞的血管生成
Fengyue Ding,Hongchun Wu,Xinglong Han et al.
Fengyue Ding et al.
Human pluripotent stem cell-derived endothelial cells (hPSC-ECs) represent a promising source of human ECs urgently needed for the study of cardiovascular disease mechanisms, cell therapy, and drug screening. This study aims to explore the ...
Transient and tunable CRISPRa regulation of APOBEC/AID genes for targeting hepatitis B virus [0.03%]
瞬时且可调的CRISPRa调控APOBEC/AID基因以靶向乙型肝炎病毒
Dmitry Kostyushev,Sergey Brezgin,Anastasiya Kostyusheva et al.
Dmitry Kostyushev et al.
APOBEC/AID cytidine deaminases play an important role in innate immunity and antiviral defenses and were shown to suppress hepatitis B virus (HBV) replication by deaminating and destroying the major form of HBV genome, covalently closed cir...
SINEUP non-coding RNA activity depends on specific N6-methyladenosine nucleotides [0.03%]
SINEUP非编码RNA的活性依赖于特异的N6-甲基腺苷碱基
Bianca Pierattini,Sabrina D&#x;Agostino,Carlotta Bon et al.
Bianca Pierattini et al.
SINEUPs are natural and synthetic antisense long non-coding RNAs (lncRNAs) selectively enhancing target mRNAs translation by increasing their association with polysomes. This activity requires two RNA domains: an embedded inverted SINEB2 el...
Synthetic miR-21 decoy circularized by tRNA splicing mechanism inhibited tumorigenesis in glioblastoma in vitro and in vivo models [0.03%]
通过tRNA拼接机制合成的miR-21假靶向环状RNA抑制胶质母细胞瘤的体内、外成瘤性
Hadi Bayat,Mohammad Hossein Pourgholami,Saeid Rahmani et al.
Hadi Bayat et al.
Glioblastoma multiforme (GBM) is the deadliest primary central nervous system tumor. miRNAs (miRs), a class of non-coding RNAs, are considered pivotal post-transcriptional regulators of cell signaling pathways. miR-21 is a reliable oncogene...
Lateral flow immunoassay for rapid and sensitive detection of dsRNA contaminants in in vitro-transcribed mRNA products [0.03%]
一种用于快速灵敏检测体外转录mRNA产品中dsRNA杂质的侧向流动免疫检测试剂盒
Dengwang Luo,Zhanfeng Wu,Daming Wang et al.
Dengwang Luo et al.
High purity is essential in mRNA-based therapeutic applications. A major contaminant of in vitro-transcribed (IVT) mRNA manufacturing is double-stranded RNA (dsRNA), which can induce severe anti-viral immune responses. Detection methods, su...
Terminal bridging of siRNA duplex at the ribose 2' position controls strand bias and target sequence preference [0.03%]
通过核糖的2'位置连接siRNA双链可以控制其链偏向性和靶序列偏好性
Atsushi Shibata,Hisao Shirohzu,Yusuke Iwakami et al.
Atsushi Shibata et al.
Small interfering RNA (siRNA) and short hairpin RNA (shRNA) are widely used as RNA interference (RNAi) reagents. Recently, truncated shRNAs that trigger RNAi in a Dicer-independent manner have been developed. We generated a novel class of R...
Proof-of-concept study for liver-directed miQURE technology in a dyslipidemic mouse model [0.03%]
用于血脂异常小鼠模型的肝靶向miQURE技术的概念验证研究
Vanessa Zancanella,Astrid Vallès,Jolanda M P Liefhebber et al.
Vanessa Zancanella et al.
A gene-silencing platform (miQURE) has been developed and successfully used to deliver therapeutic microRNA (miRNA) to the brain, reducing levels of neurodegenerative disease-causing proteins/RNAs via RNA interference and improving the dise...
Efficient transfected liposomes co-loaded with pNrf2 and pirfenidone improves safe delivery for enhanced pulmonary fibrosis reversion [0.03%]
高效转染脂质体共载pNrf2和pirfenidone改善安全递送以增强肺纤维化逆转
Xin Chang,Chang Liu,Yu-Mo Han et al.
Xin Chang et al.
Pulmonary fibrosis (PF) is an interstitial lung disease with complex pathological mechanism, and there is currently a lack of therapeutics that can heal it completely. Using gene therapy with drugs provides promising therapeutic strategies ...