A sweet barrier to gene silencing: Glucose metabolism interferes with antisense oligonucleotide therapy [0.03%]
一把双刃剑：糖代谢阻碍反义寡核苷酸疗法发挥功效
Shuangdi Duan,Weizhi Zhang,Haiming Cao
Shuangdi Duan
The biochemical function of bivalent aptamer assemblies against B cell markers CD19 and CD20 [0.03%]
靶向B细胞标记物CD19和CD20的二价适配体复合物的生化功能研究
Yilam Ng Cen,Nicole B Williams,Nicolás Di Siervi et al.
Yilam Ng Cen et al.
B cells, essential components of the adaptive immune system, are central to the pathology of B cell malignancies and autoimmune disorders. These cells can be specifically targeted using ligands that recognize B cell surface receptors such a...
Comprehensive web-based platform for advanced PCR design, genotyping, synthetic biology, molecular diagnostics, and sequence analysis [0.03%]
全面的基于Web的平台，用于先进的PCR设计、基因分型、合成生物学、分子诊断和序列分析
Ruslan Kalendar
Ruslan Kalendar
We present a comprehensive web-based platform that integrates a suite of molecular biology tools tailored for PCR-based applications. Key features include custom multiplex tiling PCR panel design for amplicon sequencing, loop-mediated isoth...
Exploring CRISPR-Cas: The transformative impact of gene editing in molecular biology [0.03%]
探索CRISPR-Cas：基因编辑在分子生物学中的变革性影响
Vivek Pandey,Shivani Sharma,Yuba Raj Pokharel
Vivek Pandey
This review traces the evolution of clustered regularly interspaced short palindromic repeats (CRISPR) technology from a prokaryotic immune mechanism to a versatile tool for precise genome engineering. We compare CRISPR with traditional gen...
mRNA vaccine based on high-frequency prevalent antigens provides broad protection against influenza B virus [0.03%]
基于高频率抗原的mRNA疫苗可为乙型流感病毒提供广谱保护
Ziqi Cheng,Yeqing Sun,Yunbo Bai et al.
Ziqi Cheng et al.
Influenza B virus (IBV) is a major pathogen affecting the human respiratory tract. B/Victoria is currently the only prevalent influenza B lineage as B/Yamagata has become increasingly rare. Existing influenza vaccines provide limited protec...
Molecular and imaging biomarker responses to brain mutant HTT lowering in a mouse model of Huntington disease [0.03%]
亨廷顿舞蹈病小鼠模型中脑部突变HTT降低的分子和影像学生物标志物反应
Nicholas S Caron,Jianjia Fan,Da Ma et al.
Nicholas S Caron et al.
Therapies targeting mutant huntingtin (mHTT) reduction in the brain hold promise as disease-modifying treatments for Huntington disease (HD), necessitating biomarkers that accurately reflect treatment response. We evaluated candidate molecu...
MicroRNA mapping of bronchial aspirate for molecular phenotyping and prognostication in patients on mechanical ventilation [0.03%]
支气管吸引物的微小核糖核酸谱型在机械通气患者的分子分型和预后中的应用
Marta Molinero,Iván D Benítez,Manel Perez-Pons et al.
Marta Molinero et al.
The application of microRNA (miRNA) profiling in respiratory biospecimens, particularly bronchial aspirate (BAS), remains underexplored. Here, we aimed to validate and refine miRNA quantification in BAS samples to establish its suitability ...
Dynamic regulation of NeuroD1 expression level by a novel viral construct during astrocyte-to-neuron reprogramming [0.03%]
新的病毒载体通过动态调节神经转录因子NeuroD1的表达水平促进星形胶质细胞重编程为功能性神经元细胞
Natalie Mseis-Jackson,Mei Jiang,Mehek Sharma et al.
Natalie Mseis-Jackson et al.
Astrocyte-to-neuron reprogramming presents a viable approach for regenerative medicine. The reprogramming factor NeuroD1 has demonstrated the capability of neuronal reprogramming with high efficiency and yet primarily glutamatergic neuronal...
Progress and prospect of minicircle as a minimized non-viral DNA vector in gene therapy and regenerative medicine [0.03%]
微创非病毒DNA载体-minicircle在基因治疗和再生医学中的进展与前景
Pegah Chanani,Naeimeh Rezaei,Kianoush Dormiani et al.
Pegah Chanani et al.
The ideal and reliable genetic treatment of human diseases relies on the use of an effective vector that does not trigger serious side effects such as immunogenicity and genotoxicity. In recent years, non-viral DNA vectors have gained signi...
Exploring exon excision as a therapeutic intervention strategy for the future treatment of ADGRV1- associated retinitis pigmentosa [0.03%]
探索外显子切除作为治疗ADGRV1相关视网膜色素变性的未来疗法策略
Merel Stemerdink,Lucija Malinar,Sanne Broekman et al.
Merel Stemerdink et al.
Given the lack of treatment options for ADGRV1-associated retinitis pigmentosa (RP), we evaluated exon excision as a potential therapeutic strategy. To enable careful selection of target exons, in silico analyses of the ADGRV1 protein domai...