Small-scale in vitro culture and purification of Plasmodium berghei for transfection experiment [0.03%]
毕氏疟原虫转染实验的小规模体外培养及提纯方法
Voravuth Somsak,Somdet Srichairatanakool,Sumalee Kamchonwongpaisan et al.
Voravuth Somsak et al.
The standard protocol for genetic modification of the rodent malaria parasite Plasmodium berghei requires infected blood from one or more laboratory mice, followed by large-scale in vitro parasite culture and purification of mature schizont...
Functional characterization of NADP-dependent isocitrate dehydrogenase isozymes from Trypanosoma cruzi [0.03%]
致病原生动物南美锥虫NADP-依赖的异柠檬酸脱氢酶同工酶的功能表征
Alejandro E Leroux,Dante A Maugeri,Juan J Cazzulo et al.
Alejandro E Leroux et al.
Trypanosoma cruzi exhibits two putative isocitrate dehydrogenases (IDHs). Both idh genes were cloned and the recombinant enzymes expressed in Escherichia coli. Our results showed that T. cruzi IDHs are strictly dependent on NADP(+) and disp...
Toxoplasma gondii toxolysin 4 is an extensively processed putative metalloproteinase secreted from micronemes [0.03%]
新分泌到细胞外的鬼轮虫虫毒酶毒素4是一种高度修饰的假定金属蛋白酶
Julie Laliberté,Vern B Carruthers
Julie Laliberté
Proteases play central roles in cell invasion by Toxoplasma gondii and other apicomplexan parasites. Herein we report the cloning and characterization of a novel secretory putative metalloproteinase, Toxolysin 4 (TLN4). T. gondii tachyzoite...
Crystal structures of three protozoan homologs of tryptophanyl-tRNA synthetase [0.03%]
三种原生动物色氨酸瑞鲍迪苷酶同系物的晶体结构
Ethan A Merritt,Tracy L Arakaki,Robert Gillespie et al.
Ethan A Merritt et al.
Tryptophanyl-tRNA synthetase (TrpRS) is an essential enzyme that is recognizably conserved across all forms of life. It is responsible for activating and attaching tryptophan to a cognate tRNA(Trp) molecule for use in protein synthesis. In ...
Mechanism of growth inhibition of intraerythrocytic stages of Plasmodium falciparum by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) [0.03%]
5-氨基咪唑-4-甲酰胺核糖核苷(AICAR)抑制疟原虫红内期生长的机制研究
Vinay Bulusu,Suman S Thakur,Roopa Venkatachala et al.
Vinay Bulusu et al.
Purine nucleotide synthesis in Plasmodium falciparum takes place solely by the purine salvage pathway in which preformed purine base(s) are salvaged from the host and acted upon by a battery of enzymes to generate AMP and GMP. Inhibitors of...
Design of anti-parasitic and anti-fungal hydroxy-naphthoquinones that are less susceptible to drug resistance [0.03%]
设计抗寄生虫和抗真菌的较少产生药物抵抗性的羟基萘醌类化合物
Louise M Hughes,Charlotte A Lanteri,Michael T O&#x;Neil et al.
Louise M Hughes et al.
Atovaquone is a hydroxy-naphthoquinone that is used to treat parasitic and fungal infections including Plasmodium falciparum (malaria), Pneumocystis jivorecii (pneumonia) and Toxoplasma gondii (toxoplasmosis). It blocks mitochondrial oxidat...
Ac-AP-12, a novel factor Xa anticoagulant peptide from the esophageal glands of adult Ancylostoma caninum [0.03%]
从犬钩口线虫食道腺中鉴定的一种新型因子Xa抗凝活性多肽AC-AP-12
Desheng Jiang,Bin Zhan,Reina S Mayor et al.
Desheng Jiang et al.
Immunoscreening an Ancylostoma caninum cDNA library with canine hookworm-infected dog serum resulted in the isolation of a 461 bp cDNA encoding Ac-AP-12, a new 9.1 kDa anticoagulant peptide (100 amino acids) with 43-69% amino acid homology ...
Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection [0.03%]
酿酒酵母二氢乳清酸脱氢酶作为恶性疟原虫转染的新选择标记基因
Suresh M Ganesan,Joanne M Morrisey,Hangjun Ke et al.
Suresh M Ganesan et al.
Genetic manipulation of Plasmodium falciparum in culture through transfection has provided numerous insights into the molecular and cell biology of this parasite. The procedure is rather cumbersome, and is limited by the number of drug-resi...
Plasmodium falciparum field isolates use complement receptor 1 (CR1) as a receptor for invasion of erythrocytes [0.03%]
恶性疟原虫野外株利用红细胞补体受体1（CR1）介导的入侵机制
Gordon A Awandare,Carmenza Spadafora,J Kathleen Moch et al.
Gordon A Awandare et al.
A majority of Plasmodium falciparum strains invade erythrocytes through interactions with sialic acid (SA) on glycophorins. However, we recently reported that complement receptor 1 (CR1) is a SA-independent invasion receptor of many laborat...
Quantitative retrotransposon anchored PCR confirms transduction efficiency of transgenes in adult Schistosoma mansoni [0.03%]
定量反转座子锚定PCR证实成年Schistosoma mansoni转基因效率
Gabriel Rinaldi,Sutas Suttiprapa,Paul J Brindley
Gabriel Rinaldi
A quantitative retrotransposon anchored PCR (qRAP) that utilizes endogenous retrotransposons as a chromosomal anchor was developed to investigate integration of transgenes in Schistosoma mansoni. The qRAP technique, which builds on earlier ...