Synthesis and Recovery of N-Sulfo Heparosan From Fermentation Broth [0.03%]
发酵滤液中N-磺代肝素低聚糖的提取与合成研究进展
Congxu Li,Xiaofeng Tang,Shuming Yuan et al.
Congxu Li et al.
Heparosan, a glycosaminoglycan (GAG) derived from bacterial capsular polysaccharide (CPS), shares a similar backbone with both clinically utilized heparin and heparan sulfate (HS). This facilitates its transformation into heparin/HS via che...
Microbial and Enzymatic Biodegradation of Polyurethane: From Depolymerization to Monomer Valorization [0.03%]
微生物和酶降解聚氨酯：从解聚到单体增值
Changlei Yu,Yuan Wen,Jiaxin Chen et al.
Changlei Yu et al.
Polyurethane (PU), as one of the most widely produced and consumed plastics globally, has led to severe environmental pollution and resource wastage due to the substantial amount of solid waste and microplastics it generates. Plastic recycl...
Deciphering Virus Removal With Ultrafiltration Membrane: Effects of Membrane Properties, Operating Conditions, and Antibody Characteristics [0.03%]
超滤膜除病毒机制研究：膜特性、运行条件及抗体特征的影响分析
Jiansheng Lu,Shilong Qi,Hui Chen et al.
Jiansheng Lu et al.
Virus filtration is a critical step to ensure the safety of antibody-based therapeutics. Due to the stringent performance requirements, commercially available virus filtration membranes are limited, and studies on virus retention behavior a...
A Fluorescent-Based Method to Assess the Activity of the Human Sodium-Coupled Citrate Transporter [0.03%]
一种基于荧光的方法来评估人类柠檬酸钠偶联转运蛋白的活性
Natali Cárcamo-Lemus,Angelo Bernier,Ivana Oporto-Ortega et al.
Natali Cárcamo-Lemus et al.
Increasing evidence supports the human sodium-coupled citrate transporter (hNaCT) as a potential therapeutic target for metabolic syndrome and early infantile epileptic encephalopathy type 25 (EIEE25). While isotopic tracers remain the refe...
Improving Aggregation Control of Recombinant Adeno-Associated Virus Serotype 2 (rAAV2) With Small Sugars and Ionic Salts [0.03%]
利用小糖和离子盐优化重组腺相关病毒2型（rAAV2）的聚集控制研究
Alexander Burns,Saurav Datta
Alexander Burns
Two persistent challenges in adeno-associated virus (AAV) manufacturing are AAV particle aggregation and the separation of full and empty AAV capsids in ion-exchange (IEX) chromatography processes, which add to AAV purification, formulation...
Synergistic Bio-Remediation of Rare Earth Mixed Contaminants in Yellow River Basin Soil Using Adsorption-Mineralization Coupling Strategy [0.03%]
黄河土地区稀土混合污染物的协同生物修复吸附矿化耦合策略研究
Lei Tian,Chi Li,De Yao et al.
Lei Tian et al.
The Yellow River Basin, as a crucial water and ecological zone, is threatened by environmental contamination from the Bayan Obo tailings dam located just 13 km away. Based on the Microbial-Induced Calcite Precipitation, this research used a...
Minor Phenolic Compounds in Detoxified Lignocellulosic Hydrolysates Are the Determinant Factor on Cell Growth and Metabolic Activity of Escherichia coli [0.03%]
乙醇梭菌解毒的木质纤维素水解物中的次要酚类化合物决定了大肠杆菌的生长和代谢活动
An Wang,Tao Han,Bin Zhang et al.
An Wang et al.
Phenolic compounds from lignin degradation in harsh pretreatment of lignocellulose persistently exist at low concentrations in hydrolysates even after standard detoxification and enzymatic hydrolysis. This study found that minor phenolic co...
Mesenchymal and Pluripotent Stem Cell-Based Strategies for Cardiac Regeneration: Mechanisms, Challenges, and Future Directions [0.03%]
基于间充质和多能干细胞的心脏再生策略：机制、挑战及未来方向
Wanying Lei,Xiuyun Wang,Jianping Cai et al.
Wanying Lei et al.
Cardiovascular diseases (CVDs) remain the predominant cause of morbidity and mortality worldwide, with limited curative treatment options for advanced heart failure (HF). While pharmacological and surgical interventions have improved surviv...
Deglycosylation of VEGF Produced in Pichia pastoris Reduces its in Vitro Binding and Angiogenic Effect [0.03%]
去除毕赤酵母表达的VEGF糖基化可降低其体外与受体结合及促管形成活性
Sibel Kalyoncu,Ceren Ozer-Cokgezme,Merve Arslan et al.
Sibel Kalyoncu et al.
Vascular endothelial growth factor (VEGF) is a key pro-angiogenic glycoprotein used in both research and therapeutic applications. While Pichia pastoris (Komagataella phaffii) provides a cost-effective expression system for recombinant VEGF...