Xylt2 Knockout Eliminates O-Xylosylation and Enhances Bone Morphogenetic Protein 2 Production in Chinese Hamster Ovary Cells [0.03%]
Xylt2基因敲除消除了O-木糖化并增强了中国仓鼠卵巢细胞中骨形态发生蛋白2的生产
Lin Han,Nana Wu,Quanxue Li et al.
Lin Han et al.
O-xylosylation of glycine-serine (GS) linkers in multispecific antibodies or fusion proteins introduces product heterogeneity, posing critical challenges for biomanufacturing quality control and elevating potential immunogenicity risks. Thi...
A Copper-Inducible Regulatory System for Dynamic Enhancement of Gene Editing Efficiency in Yarrowia lipolytica [0.03%]
一种铜离子诱导的调控系统 动态增强解脂耶氏酵母基因编辑效率
Jiacan Cao,Qing Hong,Jiaxin Sun et al.
Jiacan Cao et al.
To circumvent the potential irreversible risks to cells posed by permanent genetic modifications, this study developed a copper-inducible system for dynamically regulating gene editing efficiency of Yarrowia lipolytica. By combining promote...
Controlled Feeding Facilitates the Development of a Robust Production Process for Terpolymeric Polyhydroxyalkanoates [0.03%]
控制喂养促进耐盐假单胞菌聚(3-羟基丁酸-co-3-羟基己酸-co-4-羟基癸酸)生产过程的开发
Birk Achenbach,Pravesh Tamang,Steven Leonhardt et al.
Birk Achenbach et al.
Polyhydroxyalkanoates (PHAs) are a promising class of biodegradable and sustainable biopolymers. However, the current use of PHA is limited by its restricted availability, which is deplorable, as novel PHA variants such as copolymers or ter...
Synbiotic Supplementation With Heyndrickxia coagulans TBC169 Improves Chronic Constipation in Middle-Aged and Elderly [0.03%]
合生元（霍伯麦）改善中老年人慢性便秘疗效观察
Xiaoni Zhou,Canhui Guo,Gang Hu et al.
Xiaoni Zhou et al.
Background: Chronic constipation is prevalent in middle-aged and elderly individuals and can markedly impair quality of life and increase comorbidity risk. However, safe and effective therapies for this population remain ...
Randomized Controlled Trial
Biotechnology journal. 2026 Jun;21(6):e70250. DOI:10.1002/biot.70250 2026
Enabling Clinical Translation of Oral Probiotics Through Formulation Engineering and Scalable Manufacturing [0.03%]
通过配方工程和可扩展制造促进口腔益生菌的临床转化
Junyu Liu,Yuqi Zhang,Yuqing Huang et al.
Junyu Liu et al.
Oral probiotics, as living therapeutics, hold considerable promise for modulating host health and preventing diseases, with substantial potential for industrial and clinical applications. However, their efficacy is substantially limited by ...
Enhanced Recombinant Adeno-Associated Virus (rAAV) Biomanufacturing: Design of Experiment (DOE) Enabled Transfection Optimization for Maximum Full Capsid Yield and Robust Scale-Up [0.03%]
增强型重组腺相关病毒(rAAV)生物制造：基于实验设计的转染优化以获得最大完整衣壳产量和稳健放大的 scalability 放大上限 均为“放大”之意，但在此上下文里，scale-up一般翻译为“放大”，故采用此译名。
Alexandra Bogdanovic,Nicholas Donohue,Brian Glennon et al.
Alexandra Bogdanovic et al.
Recombinant adeno-associated virus (rAAV) gene therapies are a promising class of therapeutics. While their production via triple transfection in a single cell line offers flexibility regarding modification of the transgene, it faces signif...
High-Yield Production and Cost-Effective Purification of an Alkali-Resistant Hexameric Protein A Variant for Antibody Affinity Chromatography [0.03%]
一种耐碱的六聚体蛋白A变异体的高产生成本效益纯化及其亲和层析性能研究
Carolina Mota Natal,Claudia Lacombe,Alexander Zollner et al.
Carolina Mota Natal et al.
Recombinant Staphylococcal Protein A is the most widely used affinity ligand for industrial antibody purification, as well as for research and discovery of new antibodies. It has become a platform technology throughout the antibody manufact...
Low-Shear Circulation and Layered Regeneration: Coupled Analysis of RCCS-CFD for In Vitro Construction of Active Osteochondral Tissues [0.03%]
低剪切力循环与分层再生：RCCS-CFD体外构建活性软骨组织的联合分析
Weiting Guo,Hezhi Chen,Xueyan Hu et al.
Weiting Guo et al.
The rotating cell culture system (RCCS) is widely used in 3D in vitro culture due to its ability to provide a low shear environment and optimal mass transfer. However, current understanding of the shear stress and fluid flow distribution wi...
Yongjun Mao,Lu Bao,Gaoshuai Li et al.
Yongjun Mao et al.
Phase-separating proteins are evolutionarily conserved biomolecules that orchestrate key cellular processes. Although the biophysical principles underlying liquid-liquid phase separation (LLPS)-including multivalent interactions, intrinsica...
Engineering Secretory (S)IgA to Facilitate Affinity Purification for Large Scale Downstream Processing [0.03%]
工程型分泌性(IgA的亲和纯化以便大规模后期处理
Tim H Szeto,Julian K-C Ma
Tim H Szeto
Secretory (S)IgA class antibodies play a vital role in first line immunological defence at mucosal surfaces. Passive immunisation with monoclonal SIgA antibodies could offer a completely novel application for a biologics market currently do...