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Low-Shear Circulation and Layered Regeneration: Coupled Analysis of RCCS-CFD for In Vitro Construction of Active Osteochondral Tissues

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The rotating cell culture system (RCCS) is widely used in 3D in vitro culture due to its ability to provide a low shear environment and optimal mass transfer. However, current understanding of the shear stress and fluid flow distribution within the RCCS remain... ...