Mechanistic Insights Into the Retention and Separation Mechanism of Poly(Ethylene Glycol)-Modified Short Oligonucleotides in Anion-Exchange Chromatography [0.03%]
聚乙二醇修饰的短寡核苷酸在阴离子交换色谱保留和分离机理的见解
Noriko Yoshimoto,Tomoya Matsumoto,Yoshiatsu Ono et al.
Noriko Yoshimoto et al.
Purification is a critical step in the development of synthetic short DNA for pharmaceutical applications. PEGylation enhances stability and pharmacokinetics but introduces steric and hydration effects that affect chromatographic behavior w...
Spatiotemporal Evolution of Malignant Hepatocyte Clones Unveils Immune Evasion Features and Therapeutic Vulnerabilities in Hepatocellular Carcinoma [0.03%]
恶性肝细胞克隆的时空演化揭示了肝癌的免疫逃逸特征和治疗弱点
Shizheng Xiong,Dekang Ren,Jiaming Jin et al.
Shizheng Xiong et al.
Hepatocellular carcinoma (HCC), a highly lethal disease often developing in the context of chronic liver disease, is marked by high relapse rates and low 5-year survival. To investigate the multicellular ecosystem and molecular features of ...
Applications of Hyaluronic Acid in Pharmaceuticals, Healthcare and Cosmetics and Its Biosynthesis [0.03%]
透明质酸在医药、健康和化妆品行业的应用及其生物合成
Seyedali Mousavi,Divakar Dahiya,Poonam Singh Nigam et al.
Seyedali Mousavi et al.
Hyaluronic acid (HA) is an important homopolysaccharide also known as a natural anionic mucopolysaccharide or glycosaminoglycan formed by the alternate connection of glucuronic acid and N-acetylglucosamine as disaccharide units. For this re...
Mechanisms and Therapeutic Potential of Stem Cell-Derived Extracellular Vesicles as Nanocarriers in Musculoskeletal Disorders [0.03%]
间充质干细胞源性外泌体在骨科疾病治疗中的机制和应用前景研究进展
Mengran Qin,Guang Li,Yan Wang et al.
Mengran Qin et al.
The musculoskeletal system consists of bones, joint cartilage, tendons, ligaments, muscles, and their associated nerves and blood vessels. These components work together to maintain human movement and mechanical stability, serving as the cr...
Engineering of High-Yield Recombinant Adeno-Associated Virus Producer Plasmids [0.03%]
高效重组腺相关病毒制备质粒的构建
Marco T Radukic,Dinh To Le,Robert Freudenberg et al.
Marco T Radukic et al.
Recombinant Aadeno-associated virus (rAAV) production lags demand with respect to quality and quantity. We report insights from producer plasmid engineering aimed at increasing yield and homogeneity of rAAV vectors obtained by HEK-293 tripl...
Advanced Scaffold-Guided Stem Cell Therapies for Myocardial Regeneration: Integrating Biomaterials, Cell Engineering, and Smart Delivery Systems [0.03%]
先进的支架引导干细胞治疗心肌再生：整合生物材料、细胞工程和智能递送系统
Khadije Yousefi,Ali Zareian Jahromi,Rozhin Kasiri et al.
Khadije Yousefi et al.
Myocardial infarction remains a leading cause of mortality worldwide, primarily due to the limited regenerative capacity of adult cardiac tissue. Recent advances in regenerative medicine aim to address this limitation through stem cell ther...
Computational Fluid Dynamic Analysis of Fluid Motion and Volumetric Gas-Liquid Mass Transfer in Agitated Platelet Concentrate Storage [0.03%]
搅拌条件下血小板保存袋内流动及传质的计算流体动力学分析
Dean Pym,Amanda J Davies,Jessica O Williams et al.
Dean Pym et al.
Computational fluid dynamics (CFD) offers a powerful tool in characterizing the complex biophysical environment inducing by dynamic storage conditions, providing insights often beyond the reach of conventional experimental approaches. As ou...
From Liposomes to Virosomes: Evolution of Phospholipid Nanocarriers in Drug Delivery [0.03%]
从脂质体到病毒体：药物递送中磷脂纳米载体的发展演变
Kateryna Mykhailivna Doroshenko,Oleksandr Ivanovych Shevchenko
Kateryna Mykhailivna Doroshenko
Phospholipid-based nanocarriers are an adaptable and chemically tunable class of drug delivery systems that self-assemble into bilayered vesicles due to the amphiphilic nature of phospholipids. Phospholipid-based nanocarriers can encapsulat...
Sienna P Butterfield,Fay L Saunders,Robert J White
Sienna P Butterfield
Industrial production of biologics commonly involves the integration of transgenes into the genomes of host cells, such as Chinese hamster ovary (CHO) cells. A major determinant of productivity is the epigenetic control of the transgene pro...
Enhanced Lincomycin Titer Through Knockout of GntR Family Regulator SLCG_2790 Combined With Fermentation Optimization in an Industrial Strain of Streptomyces lincolnensis L-427 [0.03%]
通过基因敲除和发酵优化提高放线菌L-427链灰霉素产量
Yinxu Zhao,Feng Xu,Qinghai Shang et al.
Yinxu Zhao et al.
In this study, lincomycin A (Lin-A), a lincosamide antibiotic primarily synthesized by Streptomyces lincolnensis, was selected as a model to demonstrate a combined genetic regulation and process engineering strategy for production improveme...