A Thermostable Multifunctional GH5 Endoglucanase From Bacillus sp. for Lignocellulosic Biomass Saccharification [0.03%]
一种来自芽孢杆菌的耐热多功能GH5内切葡聚糖酶用于木质纤维素生物质降解糖化反应
Debjyoti Ghosh,Aditi Konar,Yi Qin Gao et al.
Debjyoti Ghosh et al.
The enzymatic conversion of lignocellulosic biomass to glucose remains a bottleneck for cost‑effective biofuel production, largely due to the complexity and expense of enzymes. Here we describe BsEG2, a multifunctional GH5‑like endoglucan...
Benchmarking Ultra-Low Attachment and Photopatterned GelMA 3D Culture Platforms for Modeling Cancer Stemness in High-Grade Serous Ovarian Cancer [0.03%]
用于卵巢癌干细胞建模的超低附着性和光图案化明胶甲基丙烯酸酯（GelMA）三维培养平台的基准测试
Deren Demirel Yavuz,Ferdi Oguz,Idil Su Canitez et al.
Deren Demirel Yavuz et al.
High-grade serous ovarian cancer (HGSOC) exhibits pronounced intratumoral heterogeneity and chemoresistance, demanding experimental platforms that more accurately recapitulate stemness-driven relapse. Despite increasing use of three-dimensi...
Recombinant Expression of Chitinase CtChi70 in Komagataella phaffii for Sustainable N, N'-Diacetyl-Chitobiose Production From Shrimp Shells [0.03%]
毕赤酵母重组表达虾壳来源的几丁质酶CtChi70用于可持续生产N,N'-二乙酰 chitobiose
Qinghe Chu,Yifan Lin,Shiqing Zhang et al.
Qinghe Chu et al.
The rapid expansion of the global seafood processing industry has generated substantial quantities of shrimp shells and other chitin-rich waste. Recombinant chitinases provide a promising strategy for converting this biomass into high-value...
Functional Activity of the Lysis Protein E From Phage ID52: Dependence on SecB for Efficient Host Cell Lysis [0.03%]
溶源菌体裂解蛋白E的活性及其对SecB依赖性分析
Lan Feng,Jing Fang,Yue Xu et al.
Lan Feng et al.
Single gene encoded phage lysis proteins offer a promising strategy for bacterial ghost production, yet their host-dependent regulatory mechanisms remain poorly understood. Here, we investigated the lysis protein E from phage ID52 (ID52-E),...
Immune Microenvironment Engineering for Functional Periodontal Regeneration: Mechanisms, Biomaterial Strategies, and Translational Perspectives [0.03%]
用于功能型牙周组织再生的免疫微环境重塑：机制、生物材料策略与转化应用前景
Ruyi Zhang,Huachang Zhang,Wenhua Zeng et al.
Ruyi Zhang et al.
Periodontitis is a chronic inflammatory disease characterized by irreversible destruction of alveolar bone, periodontal ligament attachment, and supporting tooth structures. Emerging evidence suggests that periodontal tissue breakdown and r...
Synthetic Biology of Sclareol: From the Plant Biosynthetic Pathway to Engineered Microbial and Photosynthetic Chassis [0.03%]
合成草苔烯的合成生物学：从植物生物合成途径到工程微生物和光合底盘细胞
Yichen Li,Lidan Ye,Hongwei Yu
Yichen Li
Sclareol is a highly valued bicyclic diterpene widely used as a precursor for the fragrance ambroxide and has also attracted interest as a bioactive natural product. Traditional production relies heavily on plant extraction, primarily from ...
Adaptive Evolution Identifies MHY1-Mediated Transcriptional Reprogramming for Polyphenol Tolerance in Yarrowia lipolytica [0.03%]
芽生酒酵母适应性进化鉴定出通过MHY1介导的转录重编程提高多酚耐受性
Hongyi Zhou,Md Abuhena,Zhihui Shi et al.
Hongyi Zhou et al.
Polyphenols are bioactive compounds used in food, beverages, cosmetics, and medicine. Despite their expanding use, the mechanisms of polyphenol toxicity in microbial polyphenol producers and the adaptive strategies that confer tolerance, re...
Progress and Prospects of Nanodelivery Systems in Metabolism-Regulated mRNA Vaccines [0.03%]
代谢调控型mRNA疫苗的纳米递送系统进展与前景
Wanqing Wang,Hao Zhang,Shujuan Ren et al.
Wanqing Wang et al.
mRNA vaccines face two major challenges in tumor immunotherapy: inefficient delivery and an immunosuppressive tumor microenvironment (TME) driven by metabolic reprogramming. This review proposes that nanodelivery systems can overcome both o...
Rocking-Induced Rapid Formation of Tumor Spheroids for Scalable 3D Tumor-Immune Coculture Assays [0.03%]
体外肿瘤球三维培养及免疫相互作用评估模型研究进展
Pranav Joshi,Manav Goud Vanga,Charishma Jonnadula et al.
Pranav Joshi et al.
Tumor spheroids are widely used to model the tumor microenvironment and evaluate immune-mediated cytotoxicity, but conventional methods such as ultralow-attachment and hanging-drop plates typically require 48-72 h to generate stable spheroi...
Modulating Membrane Surface Properties via Prewetting With Polysorbate 20 to Improve Sterile Filtration of Nanoemulsions [0.03%]
使用聚山梨酯20预湿调节膜表面性质以改善纳米乳的除菌过滤性能
Shreya Kapila,Milea M Osmanski,Randal J Soukup et al.
Shreya Kapila et al.
Nanoemulsions are promising vaccine adjuvants and drug delivery vehicles. However, sterile filtration of nanoemulsion formulations remains challenging because of the large size of the nanodroplets. This study investigates how prewetting the...