Advancing Raman Calibration: Automated Data Generation, Monitoring, and Control in Multi-Parallel Perfusion Mini Bioreactors [0.03%]
拉曼校准的进展：多平行灌流迷你生物反应器中的自动化数据生成、监测和控制
Rafael Machleid,Maximilian Goertz,Christian Grimm et al.
Rafael Machleid et al.
The biopharmaceutical industry is transitioning towards more efficient and cost-effective production methods, driven by the need for more affordable treatments. Process intensifications techniques, such as perfusion are the means by which t...
Transformer-Based Prediction of Sec- and Tat-Type Signal Peptides for Enhanced Bacterial Protein Secretion [0.03%]
基于变压器的Sec型和Tat型信号肽预测以增强细菌蛋白分泌
Seongmo Kang,Seong Min Lee,Ryu Hong Park et al.
Seongmo Kang et al.
Designing signal peptides (SPs) for efficient recombinant protein secretion remains challenging, as current approaches depend largely on labor-intensive screening. We developed a transformer-based model trained on 158,768 SP-protein pairs f...
Multi-Level Engineering of Bacillus Subtilis for High-Level Extracellular Production of Functional Expansins [0.03%]
用于高效分泌功能型膨胀素的枯草芽孢杆菌多级代谢工程菌株改造
Fang Luan,Yangyang Li,Xianhao Xu et al.
Fang Luan et al.
Expansins, whose cell wall-loosening function contributes to plant cell growth, biomass deconstruction, and substrate accessibility, have attracted increasing attention in agricultural biotechnology and bioprocessing. However, their predomi...
Mannose-Modified Zif-8 Nanoparticles Promote Macropinocytosis-Mediated Dendritic Cell Uptake and Induce Potent Humoral/Cellular Immunity Against Klebsiella pneumoniae [0.03%]
甘露糖修饰的ZIF-8纳米颗粒促进巨噬细胞通过巨吞作用摄取并诱导抗肺炎克雷伯氏菌的强力体液/细胞免疫反应
Gaowei Hu,Jingyi Yang,Yumeng Li et al.
Gaowei Hu et al.
Zeolitic imidazolate framework-8 (ZIF-8) has limitations in vaccine delivery, including poor targeting and rapid clearance. The outer membrane phosphoporin pore protein (PhoE) has been validated as a promising antigen against Klebsiella pne...
Phage Display-Based Discovery of HPV Capsomere-Specific Antibodies for HPV Process Characterization [0.03%]
基于噬菌体展示的HPV衣壳聚特异抗体发现及其用于HPV工艺表征的研究
Ayotunde Paul Ikujuni,Christine Banos,Sara Daley et al.
Ayotunde Paul Ikujuni et al.
Human papillomaviruses (HPV) are a diverse group of viruses that primarily infect squamous epithelial cells, often leading to proliferative lesions and various cancers. The HPV capsid is formed from the coassembly of two major capsid protei...
Enhanced Heterologous Production of Tylosin Through Pathway Reconstruction and Integrated Optimization in Streptomyces [0.03%]
通过途径重构和集成优化在链霉菌中增强泰利霉素的异源合成
Qianqian Song,Xiangyu He,Yulin Long et al.
Qianqian Song et al.
Tylosin is an important macrolide antibiotic widely used in veterinary medicine. Efficient heterologous biosynthesis can improve its biosynthesis efficiency and develop analogs with enhanced therapeutic activity. In this study, we successfu...
An Optimized Strategy for Random Peptide Library Construction Facilitates Screening of HBc-Binding Peptides via Yeast Surface Display [0.03%]
一种优化的随机肽库构建策略可促进HBc结合肽筛选
Jin-Lan Wang,Yu-Zhu Hu,Jie Li et al.
Jin-Lan Wang et al.
Peptides have been widely used in therapeutic, diagnostic, and biotechnological applications due to their high affinity, specificity, and reduced immunogenicity compared to proteins, as well as superior potency, selectivity, and lower off-t...
From Structural Complementarity to Functional Synergy: Advantages and Challenges of Combining Demineralized Dentin Matrix and Hydrogels for Bone Defect Repair [0.03%]
从结构互补到功能协同作用：脱矿牙本质基质和水凝胶结合修复骨缺损的优势与挑战
Bochen Guo,Yifan Wang,Jie Gao et al.
Bochen Guo et al.
Demineralized dentin matrix (DDM) provides intrinsic bioactivity and osteoinductive cues for bone regeneration but is limited by its rigidity and material heterogeneity. Advances in hydrogel engineering-encompassing biomimetic polymers and ...
Engineered Probiotic Saccharomyces boulardii Produces Functional Insulin With Long-Acting Effect in Mice Model [0.03%]
工程酵母酿酒活性干酵母产生功能性胰岛素并用于小鼠模型中研究其长效作用机制
Jefferson J Feng,Di Yu,Lydia Nyasae et al.
Jefferson J Feng et al.
Diabetes mellitus affects over 500 million people globally, with current insulin therapies relying on subcutaneous injections that compromise the liver's natural role in glucose regulation and suffer from poor patient compliance. Oral insul...
Human Umbilical Cord Mesenchymal Stem Cell-Derived Extracellular Vesicles Loaded with Emodin Alleviate Intestinal Injury in Acute Pancreatitis [0.03%]
人源脐带间充质干细胞源性外泌体装载黄连素可减轻急性胰腺炎相关肠损伤
Siyao Liu,Zhihong Xu,Xiong Liu et al.
Siyao Liu et al.
Acute pancreatitis (AP)-induced intestinal barrier disruption drives fatal systemic complications. We engineered human umbilical cord mesenchymal stem cell-derived extracellular vesicles (hUC-MSC-EVs) to deliver emodin-a bioactive compound ...