Insights into the catalytic mechanism of formate dehydrogenases from different microbial sources [0.03%]
不同微生物来源的一元醇脱氢酶催化机制的洞察
Laura Legnani,Marco Gargiulo,Elia Lio et al.
Laura Legnani et al.
Four formate dehydrogenases (FDHs) from Pseudomonas sp. 101, Myceliophthora thermophila, Chaetomium thermophilum, and Ogataea parapolymorpha were recombinantly produced, purified, and characterized to investigate their catalytic properties ...
GCN2 in proteostasis: structural logic, signalling networks and disease [0.03%]
GCN2在蛋白质稳态中的作用：结构逻辑、信号网络和疾病
JiaYi Zhu,Stefan J Marciniak
JiaYi Zhu
Proteostasis is the finely tuned balance of protein synthesis, folding and degradation essential for cellular health. When this equilibrium is disrupted, misfolded proteins accumulate, triggering adaptive stress responses such as the unfold...
Aurora B kinase phosphorylates keratin 8 at the cleavage furrow to drive spatially restricted intermediate filament disassembly during cytokinesis [0.03%]
极光B激酶在分裂沟中通过磷酸化角蛋白8介导细胞质分裂期间中间纤维的位点特异性拆解
Valentina Rossio,Joao A Paulo
Valentina Rossio
Keratins form intermediate filament networks that stabilize epithelial cells and many cancer cells. During cytokinesis, these filaments must be locally disassembled at the cleavage furrow to allow furrow ingression and cell separation. In t...
Crosstalk between the extracellular matrix and breast stem cells in health and disease [0.03%]
健康与疾病状态下的基底膜与乳腺肿瘤干细胞的相互作用
Giuliana Siragusa,Lorena Fernandez-de-Larrea,Niels Meesters et al.
Giuliana Siragusa et al.
Female breast development occurs at puberty and undergoes many cyclic changes under normal physiological conditions, like pregnancy, lactation and involution. The breast epithelium is surrounded by a heterogenous stroma that encompasses var...
Elucidating the catalytic mechanism of a previously undescribed carbohydrate esterase family 7 acetyl xylan esterase (LaCE7A) [0.03%]
阐明之前未描述的碳水化合物酯酶家族7木聚糖乙酰酯酶（LaCE7A）的催化机制
Ang Lv,Junwen Ma,Ling Wang et al.
Ang Lv et al.
Acetyl xylan esterases (AcXEs) are crucial for biomass degradation. However, the catalytic mechanism underlying the highly specific activity remains poorly understood, limiting their rational engineering. Here, we characterized the previous...
A new branch of mammalian vitamin B6 metabolism: AKR1C-mediated conversion of pyridoxal to pyridoxine and 4-pyridoxolactone [0.03%]
维生素B6代谢的一个新分支：AKR1C介导的吡哆醛转化为吡哆醇和4-吡哆醇 lactone
Nayu Kito,Yasuyuki Kitaura,Kyoka Iino et al.
Nayu Kito et al.
Pyridoxal 5'-phosphate (PLP), the coenzyme form of vitamin B6, is indispensable for diverse metabolic processes, especially amino acid metabolism. In mammals, PLP is primarily synthesized via a salvage pathway involving pyridoxal kinase (PL...
Siyu Li,Ziwei Liu,Jian-Wen Huang et al.
Siyu Li et al.
ABA3 proteins are a group of terpenoid cyclases (TCs), which share very low sequence identity with known proteins and lack signature Mg2+ ion-binding motifs used to define TCs. The first characterised ABA3 from Botrytis cinerea catalyses th...
Unveiling the influence of salt concentration on the different stages of the catalytic cycle of a halophilic enzyme [0.03%]
揭秘盐浓度对嗜盐酶催化循环各阶段的影响
Sergio B Kaufman,Gabriel Vallejos-Baccelliere
Sergio B Kaufman
Enzymes from halophilic organisms function in near-saturating salt concentrations. To explore enzyme adaptation to these conditions and the broader effects of salt on enzyme catalysis, we examined the salt effect on the kinetics of a glucos...
Determination of the water network surrounding the type I pilus from Escherichia coli by cryo-electron microscopy [0.03%]
利用冷冻电子显微镜确定大肠杆菌I型菌毛周围水网络的位置
Tatiana E Petrova,Anatoly S Glukhov,Artem Stetsenko et al.
Tatiana E Petrova et al.
Type 1 pili are protein filamentous surface structures of Gram-negative bacteria that mediate adhesion to host and play a crucial role in infection. Here, we report the cryogenic electron microscopy structure of the type 1 pilus from uropat...
Resistance-exercise-induced stress intervenes in TGF-β signaling by cooperatively downregulating nuclear αB-crystallin and SMAD4 in human skeletal muscle fibers [0.03%]
阻力运动诱导的压力通过协同下调人骨骼肌纤维核αB-结晶蛋白和SMAD4来干预TGF-β信号通路
Kirill Schaaf,Cassandra Duda,Tihomir Kostov et al.
Kirill Schaaf et al.
Alpha-crystallin B chain (αB-crystallin; CRYAB) helps to maintain proteostasis following cellular stress. Additionally, recent studies in fibroblasts have shown its function in the transforming growth factor beta (TGF-β) pathway, where it...