A tale of two mechanisms: Clarification of the pathway for MBD4 catalyzed glycosidic bond cleavage using MD and QM/MM calculations [0.03%]
双重机制的故事——利用分子动力学和量子力学/分子力学计算阐明MBD4催化糖苷键裂解的途径
Dylan J Nikkel,Stacey D Wetmore
Dylan J Nikkel
DNA methylation to yield 5-methylcytosine (5mC) in CpG motifs plays a vital role in epigenetic regulation. However, deamination of 5mC results in canonical thymine (T) that requires methyl-CpG-binding domain protein 4 (MBD4) for repair. Thi...
Base excision repair and homologous recombination are required for prevention of a chronic DNA damage response in Saccharomyces cerevisiae [0.03%]
碱基切除修复和同源重组是酵母中防止慢性DNA损伤反应所必需的
Yogesh Nepal,Sanjida Ahmed,Armand M Berry et al.
Yogesh Nepal et al.
The chromosomes within eukaryotic cells experience many types of damage that are generated naturally via endogenous processes. The specific pathways that are most critical for repair of such endogenously produced DNA lesions have not been i...
Stepwise DNA damage and repair mechanisms at replication forks in response to topoisomerase I inhibition [0.03%]
拓扑异构酶I抑制所引发的复制叉上DNA损伤及修复的逐步机制研究
Sofie Østergård Bæk,Kristina Keuper,Giacomo Milletti et al.
Sofie Østergård Bæk et al.
Camptothecin (CPT) and its derivative irinotecan inhibit DNA topoisomerase I (TOP1), inducing replication stress by stabilizing the TOP1 cleavage complex. This prevents DNA re-ligation, resulting in single-stranded breaks that, if unresolve...
Aditya Sethi,María Fernández-Casañas,Billie Delpino et al.
Aditya Sethi et al.
DNA secondary structures, such as hairpins, cruciforms, triplexes, G-quadruplexes and iMotifs, are common, dynamic features that replication forks routinely encounter. However, how these structures destabilise the replication fork remains u...
Fueling for the finish line: Control of human telomerase activity by nucleotide metabolism [0.03%]
通往终点线的燃料：核苷酸代谢控制人类端粒酶活性
William Mannherz,Suneet Agarwal
William Mannherz
Telomeres are repetitive DNA sequences that preserve genome integrity. Human telomere length is kept in a tight window through a balance between telomere erosion during genome replication and telomere elongation by the telomerase reverse tr...
Recent advances in understanding the molecular mechanisms of SLX4 recruitment in the replication stress response [0.03%]
近期发现的复制胁迫反应中SLX4招募分子机制的进展
Takuma Okano,Minoru Takata,Masatoshi Fujita et al.
Takuma Okano et al.
Although DNA replication is tightly regulated, various impediments can stall DNA replication forks. SLX4 is a scaffold protein that responds to different types of replication stress. While the yeast Slx4 interacts mainly with structure-spec...
Sagun Jonchhe,Sudipta Lahiri,Eli Rothenberg
Sagun Jonchhe
Guanine-rich regions in the human genome have the intrinsic ability to fold into G-quadruplex (G4) secondary structures, stabilized by stacked guanine quartets. There is considerable biochemical and structural evidence demonstrating formati...
MCM8/9 and FANCD2 interact within a shared pathway in response to replication stress caused by DNA crosslinks [0.03%]
MCM8/9和FANCD2在由DNA交联引起的复制压力下的相互作用及共同途径
Rashini Y Beragama Arachchi,Desmond C Okafor,Andrew J Snyder et al.
Rashini Y Beragama Arachchi et al.
The Fanconi anemia (FA) protein FANCD2, and MCM8/9 heterohexameric helicase complex are critical for maintaining genomic integrity in response to replication stress, however, the nature of their relationship remains unclear. Here, we show t...
Overcoming natural replication barriers formed by DNA structures and the role of repositioning to the nuclear periphery [0.03%]
克服由DNA结构形成的自然复制障碍及其重新定位到核周区的作用
Jan Leendert Boer,Tyler M Maclay,Nolan T Caile et al.
Jan Leendert Boer et al.
Endogenous barriers to DNA replication, such as repetitive DNA, non-B DNA structures, and protein barriers present significant challenges to replication. Upon encountering one of these barriers, cells employ a number of strategies to ensure...
Abigayle F Vito,Daniel J Boesch,Ava M Hammons et al.
Abigayle F Vito et al.
Base excision repair (BER) is a genome surveillance pathway responsible for repairing DNA base lesions distributed throughout the chromatinized eukaryotic genome. However, chromatin structure acts as a dynamic structural barrier that restri...