Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels [0.03%]
转录起始位点选择的定量分析显示其受DNA序列、RNA聚合酶II活性和NTP水平控制
Yunye Zhu,Irina O Vvedenskaya,Sing-Hoi Sze et al.
Yunye Zhu et al.
Transcription start site (TSS) selection is a key step in gene expression and occurs at many promoter positions over a wide range of efficiencies. Here we develop a massively parallel reporter assay to quantitatively dissect contributions o...
CAND1 inhibits Cullin-2-RING ubiquitin ligases for enhanced substrate specificity [0.03%]
CAND1通过抑制Cul2-RING泛素连接酶复合物来提高底物特异性
Kankan Wang,Stephanie Diaz,Lihong Li et al.
Kankan Wang et al.
Through targeting essential cellular regulators for ubiquitination and serving as a major platform for discovering proteolysis-targeting chimera (PROTAC) drugs, Cullin-2 (CUL2)-RING ubiquitin ligases (CRL2s) comprise an important family of ...
Co-opting the E3 ligase KLHDC2 for targeted protein degradation by small molecules [0.03%]
小分子通过借用E3连接酶KLHDC2来实现目标蛋白质的降解
Christopher M Hickey,Katherine M Digianantonio,Kurt Zimmermann et al.
Christopher M Hickey et al.
Targeted protein degradation (TPD) by PROTAC (proteolysis-targeting chimera) and molecular glue small molecules is an emerging therapeutic strategy. To expand the roster of E3 ligases that can be utilized for TPD, we describe the discovery ...
General transcription factor from Escherichia coli with a distinct mechanism of action [0.03%]
具有独特作用机制的大肠杆菌通用转录因子
Nikita Vasilyev,Mengjie M J Liu,Vitaly Epshtein et al.
Nikita Vasilyev et al.
Gene expression in Escherichia coli is controlled by well-established mechanisms that activate or repress transcription. Here, we identify CedA as an unconventional transcription factor specifically associated with the RNA polymerase (RNAP)...
Reduction of DHHC5-mediated beclin 1 S-palmitoylation underlies autophagy decline in aging [0.03%]
DHHC5介导的自噬在衰老过程中贝克莱因1S-棕榈酰化降低
Rui Guo,Jianping Liu,Xia Min et al.
Rui Guo et al.
Autophagy is a lysosome-dependent degradation pathway essential for cellular homeostasis, which decreases with age. However, it is unclear how aging induces autophagy decline. Here we show the role of protein S-palmitoylation in autophagy. ...
THEMIS is a substrate and allosteric activator of SHP1, playing dual roles during T cell development [0.03%]
THEMIS是SHP1的底物和变构激活剂，在T细胞发育过程中发挥双重作用
Jiali Zhang,Zhenzhou Jiang,Xueyuan Zhang et al.
Jiali Zhang et al.
THEMIS plays an indispensable role in T cells, but its mechanism of action has remained highly controversial. Using the systematic proximity labeling methodology PEPSI, we identify THEMIS as an uncharacterized substrate for the phosphatase ...
Structural insight into the assembly and working mechanism of helicase-primase D5 from Mpox virus [0.03%]
猴痘病毒解旋酶引物酶D5的组装及工作机制的结构见解
Yaning Li,Jing Zhu,Yingying Guo et al.
Yaning Li et al.
The Mpox pandemic, caused by the Mpox virus (or monkeypox virus, MPXV), has gained global attention. The D5 protein, a putative helicase-primase found in MPXV, plays a vital role in viral replication and genome uncoating. Here we determined...
Structural basis of the human NAIP/NLRC4 inflammasome assembly and pathogen sensing [0.03%]
人类NAIP/NLRC4炎性小体组装及病原体感知的结构基础
Rosalie E Matico,Xiaodi Yu,Robyn Miller et al.
Rosalie E Matico et al.
The NLR family caspase activation and recruitment domain-containing 4 (NLRC4) inflammasome is a critical cytosolic innate immune machine formed upon the direct sensing of bacterial infection and in response to cell stress during sterile chr...
Kevin Felt,Madeleine Stauffer,Leslie Salas-Estrada et al.
Kevin Felt et al.
Hyperactivity of serotonin 3 receptors (5-HT3R) underlies pathologies associated with irritable bowel syndrome and chemotherapy-induced nausea and vomiting. Setrons, a class of high-affinity competitive antagonists, are used in the treatmen...
Auto-suppression of Tet dioxygenases protects the mouse oocyte genome from oxidative demethylation [0.03%]
四氢二氧杂环化合物的自动抑制保护了小鼠卵母细胞基因组免受氧化去甲基化的影响
Xiao-Jie Zhang,Bin-Bin Han,Zhen-Yu Shao et al.
Xiao-Jie Zhang et al.
DNA cytosine methylation plays a vital role in repressing retrotransposons, and such derepression is linked with developmental failure, tumorigenesis and aging. DNA methylation patterns are formed by precisely regulated actions of DNA methy...