Structural basis of the subcortical maternal complex and its implications in reproductive disorders [0.03%]
皮质下母体复合物的结构基础及其在生殖障碍中的意义
Pengliang Chi,Guojin Ou,Dandan Qin et al.
Pengliang Chi et al.
The subcortical maternal complex (SCMC) plays a crucial role in early embryonic development. Malfunction of SCMC leads to reproductive diseases in women. However, the molecular function and assembly basis for SCMC remain elusive. Here we re...
Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes [0.03%]
转录诱导的域形成细菌染色体的基本约束构建块
Amaury Bignaud,Charlotte Cockram,Céline Borde et al.
Amaury Bignaud et al.
Transcription generates local topological and mechanical constraints on the DNA fiber, leading to the generation of supercoiled chromosome domains in bacteria. However, the global impact of transcription on chromosome organization remains e...
Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM [0.03%]
深度诱变和冷冻电镜揭示的clamp-loader ATP酶的自动抑制作用
Kendra Marcus,Yongjian Huang,Subu Subramanian et al.
Kendra Marcus et al.
Clamp loaders are AAA+ ATPases that facilitate high-speed DNA replication. In eukaryotic and bacteriophage clamp loaders, ATP hydrolysis requires interactions between aspartate residues in one protomer, present in conserved 'DEAD-box' motif...
David Moses,Karina Guadalupe,Feng Yu et al.
David Moses et al.
Intrinsically disordered proteins and protein regions (IDPs) are prevalent in all proteomes and are essential to cellular function. Unlike folded proteins, IDPs exist in an ensemble of dissimilar conformations. Despite this structural plast...
PtuA and PtuB assemble into an inflammasome-like oligomer for anti-phage defense [0.03%]
PtuA和PtuB组装成类似炎症小体的寡聚物以抵御噬菌体攻击
Yuanyuan Li,Zhangfei Shen,Mengyuan Zhang et al.
Yuanyuan Li et al.
Escherichia coli Septu system, an anti-phage defense system, comprises two components: PtuA and PtuB. PtuA contains an ATPase domain, while PtuB is predicted to function as a nuclease. Here we show that PtuA and PtuB form a stable complex w...
Structural insights into the activation and inhibition of CXC chemokine receptor 3 [0.03%]
CXC趋化因子受体3的激活和抑制作用的结构研究
Haizhan Jiao,Bin Pang,Aijun Liu et al.
Haizhan Jiao et al.
The chemotaxis of CD4+ type 1 helper cells and CD8+ cytotoxic lymphocytes, guided by interferon-inducible CXC chemokine 9-11 (CXCL9-11) and CXC chemokine receptor 3 (CXCR3), plays a critical role in type 1 immunity. Here we determined the s...
Koya Sakuma,Naohiro Kobayashi,Toshihiko Sugiki et al.
Koya Sakuma et al.
A wide range of de novo protein structure designs have been achieved, but the complexity of naturally occurring protein structures is still far beyond these designs. Here, to expand the diversity and complexity of de novo designed protein s...
Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis [0.03%]
Aurora激酶A介导的磷酸化作用引发Rab1A结构改变以增强有丝分裂期间的内质网复杂性
Wei Zhang,Zijian Zhang,Yun Xiang et al.
Wei Zhang et al.
Morphological rearrangement of the endoplasmic reticulum (ER) is critical for metazoan mitosis. Yet, how the ER is remodeled by the mitotic signaling remains unclear. Here, we report that mitotic Aurora kinase A (AURKA) employs a small GTPa...
The Ruminococcus bromii amylosome protein Sas6 binds single and double helical α-glucan structures in starch [0.03%]
Ruminococcus bromii的淀粉酶体蛋白Sas6能结合淀粉中的单个和双链的α-葡聚糖结构
Amanda L Photenhauer,Rosendo C Villafuerte-Vega,Filipe M Cerqueira et al.
Amanda L Photenhauer et al.
Resistant starch is a prebiotic accessed by gut bacteria with specialized amylases and starch-binding proteins. The human gut symbiont Ruminococcus bromii expresses Sas6 (Starch Adherence System member 6), which consists of two starch-speci...
DNA methylation restricts coordinated germline and neural fates in embryonic stem cell differentiation [0.03%]
DNA甲基化限制了胚胎干细胞分化中的生殖系和神经命运的协调
Mathieu Schulz,Aurélie Teissandier,Elena De La Mata Santaella et al.
Mathieu Schulz et al.
As embryonic stem cells (ESCs) transition from naive to primed pluripotency during early mammalian development, they acquire high DNA methylation levels. During this transition, the germline is specified and undergoes genome-wide DNA demeth...