A novel class of inhibitors that disrupts the stability of integrin heterodimers identified by CRISPR-tiling-instructed genetic screens [0.03%]
由CRISPR tiles指导的基因筛选鉴定出的一类新的可破坏整合素异二聚体稳定性的抑制剂
Nicole M Mattson,Anthony K N Chan,Kazuya Miyashita et al.
Nicole M Mattson et al.
The plasma membrane is enriched for receptors and signaling proteins that are accessible from the extracellular space for pharmacological intervention. Here we conducted a series of CRISPR screens using human cell surface proteome and integ...
Conformational ensemble of yeast ATP synthase at low pH reveals unique intermediates and plasticity in F1-Fo coupling [0.03%]
酵母ATP合成酶在低pH下的构象集合揭示了独特的中间体和F1-Fo耦合的可塑性
Stuti Sharma,Min Luo,Hiral Patel et al.
Stuti Sharma et al.
Mitochondrial adenosine triphosphate (ATP) synthase uses the proton gradient across the inner mitochondrial membrane to synthesize ATP. Structural and single molecule studies conducted mostly at neutral or basic pH have provided details of ...
Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair [0.03%]
转录偶联修复中RNA聚合酶II泛素化和失活的结构基础
Goran Kokic,George Yakoub,Diana van den Heuvel et al.
Goran Kokic et al.
During transcription-coupled DNA repair (TCR), RNA polymerase II (Pol II) transitions from a transcriptionally active state to an arrested state that allows for removal of DNA lesions. This transition requires site-specific ubiquitylation o...
The palisade layer of the poxvirus core is composed of flexible A10 trimers [0.03%]
痘病毒核心的栅栏层由灵活的A10三聚体组成
Jiasui Liu,Simon Corroyer-Dulmont,Vojtěch Pražák et al.
Jiasui Liu et al.
Due to its asymmetric shape, size and compactness, the structure of the infectious mature virus (MV) of vaccinia virus (VACV), the best-studied poxvirus, remains poorly understood. Instead, subviral particles, in particular membrane-free vi...
Multi-modal cryo-EM reveals trimers of protein A10 to form the palisade layer in poxvirus cores [0.03%]
多模式低温电镜揭示A10蛋白三聚体形成痘病毒核心的栅栏层结构
Julia Datler,Jesse M Hansen,Andreas Thader et al.
Julia Datler et al.
Poxviruses are among the largest double-stranded DNA viruses, with members such as variola virus, monkeypox virus and the vaccination strain vaccinia virus (VACV). Knowledge about the structural proteins that form the viral core has remaine...
Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport [0.03%]
肌球蛋白1-I / C在oskar mRNA转运过程中协调驱动蛋白-1和动力蛋白电机
Simone Heber,Mark A McClintock,Bernd Simon et al.
Simone Heber et al.
Dynein and kinesin motors mediate long-range intracellular transport, translocating towards microtubule minus and plus ends, respectively. Cargoes often undergo bidirectional transport by binding to both motors simultaneously. However, it i...
Structures of complete extracellular receptor assemblies mediated by IL-12 and IL-23 [0.03%]
由IL-12和IL-23介导的完整细胞外受体复合物的结构研究
Yehudi Bloch,Jan Felix,Romain Merceron et al.
Yehudi Bloch et al.
Cell-surface receptor complexes mediated by pro-inflammatory interleukin (IL)-12 and IL-23, both validated therapeutic targets, are incompletely understood due to the lack of structural insights into their complete extracellular assemblies....
The structure of a human translation initiation complex reveals two independent roles for the helicase eIF4A [0.03%]
人类翻译起始复合体的结构揭示了解旋酶eIF4A的两个独立作用
Jailson Brito Querido,Masaaki Sokabe,Irene Díaz-López et al.
Jailson Brito Querido et al.
Eukaryotic translation initiation involves recruitment of the 43S pre-initiation complex to the 5' end of mRNA by the cap-binding complex eIF4F, forming the 48S translation initiation complex (48S), which then scans along the mRNA until the...
Variant in the synaptonemal complex protein SYCE2 associates with pregnancy loss through effect on recombination [0.03%]
黏轴蛋白复合体蛋白SYCE2的变异通过影响重组而导致妊娠丧失
Valgerdur Steinthorsdottir,Bjarni V Halldorsson,Hakon Jonsson et al.
Valgerdur Steinthorsdottir et al.
Two-thirds of all human conceptions are lost, in most cases before clinical detection. The lack of detailed understanding of the causes of pregnancy losses constrains focused counseling for future pregnancies. We have previously shown that ...
Eva Fortea,Sangyun Lee,Rahul Chadda et al.
Eva Fortea et al.
CLCs are dimeric chloride channels and anion/proton exchangers that regulate processes such as muscle contraction and endo-lysosome acidification. Common gating controls their activity; its closure simultaneously silences both protomers, an...