Gel purification of genomic DNA removes contaminating small DNA fragments interfering with polymerase chain reaction analysis of small fragment homologous replacement [0.03%]
凝胶提取基因组DNA可除去污染的短片段DNA提高小型同源重组分析的聚合酶链式反应的特异性与灵敏度
Rosalie Maurisse,Yann Fichou,David De Semir et al.
Rosalie Maurisse et al.
Oligonucleotides can mediate sequence-specific gene modification that results in the correction and/or alteration of genomic DNA. There is evidence to suggest that the polymerase chain reaction (PCR)-based analytical methods usually used to...
Suppression of tumor growth using antisense oligonucleotide against survivin in an orthotopic transplant model of human hepatocellular carcinoma in nude mice [0.03%]
在裸鼠人肝癌原位移植模型中采用反义寡核苷酸抑制Survivin基因表达并观察肿瘤生长抑制作用
Yuning Sun,Ruxian Lin,Jinming Dai et al.
Yuning Sun et al.
Survivin, an inhibitor of apoptosis protein, deserves attention as a selective target for cancer therapy because it is overexpressed in many cancers, including human hepatocellular carcinoma (HCC). Here, we report a novel antisense oligonuc...
RNA interference from multimeric shRNAs generated by rolling circle transcription [0.03%]
通过滚环转录产生多聚体shRNA的RNA干扰作用
Attila A Seyhan,Alexander V Vlassov,Brian H Johnston
Attila A Seyhan
Methods most commonly used for producing small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) are chemical synthesis and intracellular expression from engineered vectors. For shRNAs, chemical synthesis is very costly and construct...
2'-Deoxy purine, 2'-O-methyl pyrimidine (dRmY) aptamers as candidate therapeutics [0.03%]
用于治疗的2'-脱氧嘌呤、2'-O-甲基嘧啶（dRmY）适配体候选药物
Paula E Burmeister,Chunhua Wang,Jason R Killough et al.
Paula E Burmeister et al.
Aptamers are short oligonucleotides that fold into well-defined three-dimensional architectures thereby enabling specific binding to molecular targets such as proteins. To be successful as a novel therapeutic modality, it is important for a...
Comparison of different strategies to select aptamers against a transmembrane protein target [0.03%]
不同策略选择跨膜蛋白靶标的适配体的比较
Carine Pestourie,Laura Cerchia,Karine Gombert et al.
Carine Pestourie et al.
Binding of aptamers is dependent on their target conformation, which in turn is conditioned by the target's environment. Therefore, selection of aptamers against the active forms of membrane proteins could require their correct membrane ins...
Comparative Study
Oligonucleotides. 2006 Winter;16(4):323-35. DOI:10.1089/oli.2006.16.323 2006
Immunostimulatory properties of CpG-oligonucleotides are enhanced by the use of protamine nanoparticles [0.03%]
阳离子protamine纳米粒可增强CpG寡核苷酸的免疫刺激作用
Miren Kerkmann,Dirk Lochmann,Jörg Weyermann et al.
Miren Kerkmann et al.
The aim of this paper was to investigate if the immunostimulatory effects of CpG-oligonucleotides (CpG-ODN) can be enhanced by the use of biodegradable protamine nanoparticles (proticles). We analyzed size, surface charge, and morphology of...
The DNAzymes Rs6, Dz13, and DzF have potent biologic effects independent of catalytic activity [0.03%]
DNA酶Rs6、Dz13和DzF的生物学效应独立于其催化活性而存在
Laurent Rivory,Carly Tucker,Andrew King et al.
Laurent Rivory et al.
DNAzymes are catalytic DNA molecules capable of cleaving RNA substrates and therefore constitute a possible gene-suppression technology. We examined whether the previously reported potency of a DNAzyme targeting c-jun (Dz13) could be improv...
Comparative Study
Oligonucleotides. 2006 Winter;16(4):297-312. DOI:10.1089/oli.2006.16.297 2006
Inhibition of infectious human immunodeficiency virus type 1 virions via lentiviral vector encoded short antisense RNAs [0.03%]
利用慢病毒表达的短发夹RNA阻断人类免疫缺陷病毒的感染力
Shuo Gu,Jianfei Ji,James D Kim et al.
Shuo Gu et al.
During the life cycles of most retroviruses and lentiviruses, dimerization and packaging of two copies of viral genomic RNA is required for the subsequent conversion of RNA into double stranded DNA by reverse transcriptase. For human immuno...
Immunostimulatory PyNTTTTGT oligodeoxynucleotides: structural properties and refinement of the active motif [0.03%]
免疫刺激PyNTTTTGT寡核苷酸：结构特性和活性基序的优化
Juan M Rodriguez,Fernanda Elías,Juan Fló et al.
Juan M Rodriguez et al.
It is well known that synthetic oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotides within a given context stimulate B lymphocytes and plasmacytoid dendritic cells (PDCs) of the vertebrate immune system. We have reported ...
Complete reversal of ABCG2-depending atypical multidrug resistance by RNA interference in human carcinoma cells [0.03%]
利用RNA干扰技术在人类癌细胞中实现ABCG2依赖型非典型多药耐药性的逆转
Axel Priebsch,Franziska Rompe,Holger Tönnies et al.
Axel Priebsch et al.
In the chemotherapeutic treatment of patients with disseminated neoplasms, multidrug resistance (MDR) is a major obstacle. ABCG2 (BCRP/MXR), a member of the superfamily of adenosine triphosphate-binding cassette (ABC) transporters, was demo...