Sequestering of the 3' splice site in a theophylline-responsive riboswitch allows ligand-dependent control of alternative splicing [0.03%]
肌苷响应的核糖开关通过结合依赖的方式调控选择性剪切中的3'供体剪切位点沉寂
Veronica Gusti,Dong-Suk Kim,Rajesh K Gaur
Veronica Gusti
Despite the important role of alternative splicing in various aspects of biological processes, our ability to regulate this process at will remains a challenge. In this report, we asked whether a theophylline-responsive riboswitch could be ...
Fast deprotection of synthetic oligodeoxyribonucleotides using standard reagents under microwave irradiation [0.03%]
微波照射下使用常规试剂快速去除寡核苷酸合成保护基团体积分
Adrian S Culf,Miroslava Cuperlović-Culf,Mark Laflamme et al.
Adrian S Culf et al.
Fast methods for the removal of permanent amide exo-cyclic protective groups widely used in phosphoramidite-method DNA synthesis are desirable for many genomics and proteomics applications. In this communication, we present a method for the...
Investigation of the interaction between ssDNA and 2-aminophenoxazine-3-one and development of an electrochemical DNA biosensor [0.03%]
ssDNA与2-氨基邻苯二酮相互作用研究及电化学生物传感器的构建
Xue-Mei Li,Heng-Qiang Ju,Shu-Sheng Zhang
Xue-Mei Li
An electrochemical method was used to probe the interaction between 2-aminophenoxazine-3-one (AP) and the short DNA sequence related to the hepatitis B virus (HBV), and an electrochemical DNA biosensor was developed. The voltammetric signal...
C N N&#x;soukpoé-Kossi,H A Tajmir-Riahi
C N N&#x;soukpoé-Kossi
Deoxyribonuclease I (DNase I) binds right-handed DNA duplex via a minor groove and the backbone phosphate group with no contact to the major groove. It hydrolyses double-stranded DNA predominantly by a single-stranded nicking mechanism unde...
G-rich oligonucleotides alter cell cycle progression and induce apoptosis specifically in OE19 esophageal tumor cells [0.03%]
G-富足寡核苷酸能特异性地改变OE19食道肿瘤细胞的细胞周期进程并诱导其凋亡
Timothy R Schwartz,Casey A Vasta,Thomas L Bauer et al.
Timothy R Schwartz et al.
Short synthetic oligonucleotides (ODNs) can be used to block cellular processes involved in cell growth and proliferation. Often acting as aptamers, these molecules interact with critical proteins that regulate the induction of apoptosis or...
Biodistribution of 68Ga-labeled LNA-DNA mixmer antisense oligonucleotides for rat chromogranin-A [0.03%]
68Ga标记的LNA-DNA混交体反义寡核苷酸在大鼠中的胆囊素A生物分布研究
Gabor Lendvai,Irina Velikyan,Sergio Estrada et al.
Gabor Lendvai et al.
In vivo monitoring of gene expression may be accomplished using a most advanced imaging technology such as positron emission tomography (PET). However, a range of methodological and biological hurdles needs exploration. In the present study...
Double-stranded break can be repaired by single-stranded oligonucleotides via the ATM/ATR pathway in mammalian cells [0.03%]
单链寡核苷酸可通过ATM/ATR途径修复哺乳动物细胞中的双链断裂事件
Zai Wang,Zhong-Jun Zhou,De-Pei Liu et al.
Zai Wang et al.
Single-stranded oligonucleotide (SSO)-mediated gene modification is a newly developed tool for site-specific gene repair in mammalian cells; however, the corrected cells always show G2/M arrest and cannot divide to form colonies. This pheno...
Single acute-dose and repeat-doses toxicity of anti-HIV-1 PNA TAR-penetratin conjugate after intraperitoneal administration to mice [0.03%]
抗HIV-1 PNA TAR-穿透多肽缀合物腹腔注射的单剂量和重复计量毒性研究报告
Binay Chaubey,Snehlata Tripathi,Virendra N Pandey
Binay Chaubey
Polyamide (peptide) nucleic acids conjugated with membrane-penetrating peptide are potential antisense therapeutic agents because of their unique chemical properties, high target specificity, and efficient cellular uptake. However, studies ...
Molecular design guided by a local map of sequence space: DNA aptamers that inhibit cathepsin E [0.03%]
由序列空间局部图引导的分子设计：抑制 Cathepsin E 的 DNA 配体
Chuya Yoshida,Akira Kuniwake,Mohammed Naimuddin et al.
Chuya Yoshida et al.
It has been strongly demanded by a number of people to elevate activities of molecules of a particular function. Currently, there is no general guide available for this purpose. Here we present a novel approach for this; a local sequence sp...
Zhaoyang Ye,Houssam S Hajj Houssein,Ram I Mahato
Zhaoyang Ye
Liver fibrosis results from chronic liver injury due to hepatitis B and C, excessive alcohol ingestion, and metal ion overload. Fibrosis culminates in cirrhosis and results in liver failure. Therefore, a potent antifibrotic therapy is urgen...