A method for peptide successive C-terminal degradation using dilute hydrochloric acid [0.03%]
一种使用稀盐酸对多肽进行连续C端降解的方法
M Takayama,T Matsui,T Sakai et al.
M Takayama et al.
Dilute hydrochloric acid (10% w/v, 2.74 N) was reacted with peptides and proteins at 25 degrees C for 14 days and 30 days or at 50 degrees C for 1 to 16 hours.These reactions caused successive C-terminal degradation and deamidation of C-ter...
K M Hager,J W Fox,M Gunthorpe et al.
K M Hager et al.
The Nucleic Acids Research Group of the Association of Biomolecular Resource Facilities (ABRF) last surveyed DNA synthesis core facilities in April 1995. Because of the introduction of new technologies and dramatic changes in the market, we...
Considerations in adding mutation detection services to a sequencing core facility [0.03%]
测序核心设施添加突变检测服务的考量因素
M R Bonner,L W Ballard
M R Bonner
Because analysis of single nucleotide polymorphisms (SNPs) can be invaluable in understanding genomic variation and the genetic basis of disease, there is a need for high-throughput, high-accuracy mutation detection methods for identifying ...
Analytical ultracentrifugation as a contemporary biomolecular research tool [0.03%]
分析超速离心在当代生物分子研究中的应用
J L Cole,J C Hansen
J L Cole
Analytical ultracentrifugation has again become a widely used biomolecular research technique for determining sample purity, characterizing assembly and disassembly mechanisms of biomolecular complexes, determining subunit stoichiometries, ...
An automated C-terminal sequencing method for analysis of proteins us ing an ABI 473A sequencer [0.03%]
一种利用ABI 473A序列分析仪对蛋白质进行自动C末端测序的方法
S Tang,J Mozdzanowski,K R Anumula
S Tang
We developed a novel chemistry for C-terminal sequencing of proteins based on derivatization with acetylisothiocyanate to yield amino acid thiohydantoins (TH-AAs), and it was used for manual sequencing of biopharmaceutical products on a rou...
E R Mardis
E R Mardis
Commercial availability of multicapillary DNA sequencing instruments can significantly impact the existing paradigm in high-throughput DNA sequencing facilities, shifting the rate-limiting step away from the electrophoresis and detection st...
Identification of proteins electroblotted to polyvinylidene difluoride membrane by combined amino acid analysis and bioinformatics: An ABRF multicenter study [0.03%]
结合氨基酸分析与生物信息学鉴定电泳转移至聚偏二氟乙烯膜上蛋白质的联合应用——ABRF多中心研究
P Hunziker,T T Andersen,Y Bao et al.
P Hunziker et al.
The ABRF amino acid analysis study evaluated the general utility of amino acid analysis (AAA) for identification of proteins after denaturing gel electrophoresis and electroblotting to polyvinylidene difluoride (PVDF) membrane.Thirty-eight ...
Application of proteases to the identification of chiral modifications in synthetic peptides [0.03%]
应用蛋白酶识别合成肽中的手性修饰
K M Keating
K M Keating
Racemization of amino acids during solid-phase synthesis of peptides leads to the formation of side products that are chirally modified peptides. The chiral specificity of enzymes can be exploited to identify the sites of the modifications ...
J deBlaquiere,A W Burgess
J deBlaquiere
The interaction of biotin and avidin was used to affinity purify plasma membranes for use in in vitro studies of the epidermal growth factor (EGF) receptor and other cell-surface molecules. Biotinylated mouse fibroblasts were homogenized an...
Determination of molecular masses of proteins in solution: Implementation of an HPLC size exclusion chromatography and laser light scattering service in a core laboratory [0.03%]
溶液中蛋白质分子量的测定：在中心实验室实施HPLC凝胶色谱和激光散射服务
E Folta-Stogniew,K R Williams
E Folta-Stogniew
Size exclusion chromatography (SEC) coupled with "on-line" laser light scattering (LS), refractive index (RI), and ultraviolet (UV) detection provides an elegant approach to determining the molecular weights of proteins and their complexes ...