A method for peptide successive C-terminal degradation using dilute hydrochloric acid
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Dilute hydrochloric acid (10% w/v, 2.74 N) was reacted with peptides and proteins at 25 degrees C for 14 days and 30 days or at 50 degrees C for 1 to 16 hours.These reactions caused successive C-terminal degradation and deamidation of C-terminal alpha-amide an... ...
