Determination of molecular masses of proteins in solution: Implementation of an HPLC size exclusion chromatography and laser light scattering service in a core laboratory
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Size exclusion chromatography (SEC) coupled with "on-line" laser light scattering (LS), refractive index (RI), and ultraviolet (UV) detection provides an elegant approach to determining the molecular weights of proteins and their complexes in solution. SEC ser... ...