Anton Utyushev,Ilia L Rasskazov,Yamuna Phal
Anton Utyushev
We present a rigorous electromagnetics-based theoretical model for mid-infrared (mid-IR) absorption and vibrational circular dichroism (VCD) spectra of homogeneous samples. The formalism based on 4 × 4 transfer-matrix methodology enables q...
SpermFACS: Validation of a Highly Sensitive Sperm Cell Sorting Method for Sexual Assault Casework Analysis [0.03%]
Kristina Fokias,Iene Rutten,Karen Ven et al.
Kristina Fokias et al.
The identification and isolation of sperm cells from biological samples remain a pivotal component of forensic analysis in sexual offense investigations. Although differential lysis (DL) is the standard method for sperm cell separation, it ...
Target-Induced DNA Nanodevice as Efficient Signal Amplification Strategy Coupled with a Regenerable SERS Substrate HOF@Au for Ultrasensitive Detection of Roxithromycin [0.03%]
Runzi Zhang,Shunbi Xie,Xiaoyu Yang et al.
Runzi Zhang et al.
Currently, surface-enhanced Raman scattering (SERS) biosensors face practical challenges, including SERS substrate waste and unsatisfactory detection results. Accordingly, the highly efficient and regenerable SERS substrate HOF@Au was colla...
Design and Optimization of the Interface of Gas Chromatography and Photoionization Ion Mobility Spectrometry Aided by Multiphysics Field Dynamic Convolution [0.03%]
Peiran Song,Huaiwen Cang,Zhihao Zhang et al.
Peiran Song et al.
Photoionization ion mobility spectrometry (PIMS) hyphenated with gas chromatography (GC) exhibits outstanding selectivity and high sensitivity in the detection of trace volatile organic compounds (VOCs). Its analytical performance is consid...
Development of a High-Fidelity EXPAR Platform and Its Application for Ultrasensitive Detection of Diabetic Foot Ulcer-Associated MicroRNAs [0.03%]
Ye Tian,Xinxi Li,Jingdong Tang et al.
Ye Tian et al.
Diabetic foot ulcer (DFU), a severe complication of diabetes, necessitates sensitive detection of pathogenic microRNAs (miRNAs) as emerging diagnostic biomarkers. The exponential amplification reaction (EXPAR) offers high efficiency and rap...
Yuanyi He,Zijun Ren,Xiaojie Chen et al.
Yuanyi He et al.
Cell atlases can reveal cell heterogeneity within tissues, which are inherently complex biological samples. While high-throughput single-cell metabolomics has been reported, single-cell metabolic atlases are difficult to generate without ac...
Gas Sensing Performance of Lead-Free Perovskite Cs3Sb2Cl9 for Monitoring Typical Electrolyte (DMC) Leakage in Lithium-Ion Batteries [0.03%]
Chaofan Ma,Huiyu Su,Yazhou Yang et al.
Chaofan Ma et al.
Dimethyl carbonate (DMC), the primary component of lithium-ion battery (LIBs) electrolytes, is volatile at room temperature and can serve as a key indicator for detecting electrolyte leakage. In this work, leaf-like Cs3Sb2Cl9 was synthesize...
Method-at-a-Glance (MAG) as a Graphical Approach for Method Development in Reversed-Phase Liquid Chromatography of Pharmaceuticals [0.03%]
Mahmoud Hamed,Reham Kannouma,Alaa Bedair et al.
Mahmoud Hamed et al.
Reversed-phase high-performance liquid chromatography (RP-HPLC) remains the dominant separation mode in pharmaceutical and biomedical analysis, yet method development is still largely driven by trial-and-error practices, fragmented literatu...
One-Pot CRISPR/Cas12a Assay Based on Ultrashort HDA for Ultrasensitive and Universal Nucleic Acid Detection [0.03%]
Huimin Liao,Huiyun Xie,Hengming Ye et al.
Huimin Liao et al.
Isothermal amplification techniques, such as helicase-dependent amplification (HDA) combined with CRISPR, are cutting-edge approaches for nucleic acid detection. In this work, we developed a novel ultrashort mesophilic HDA (termed usHDA) fo...
Direct RNA Triggering of Cas12a through the Native crRNA Architecture Enables Clinical Nucleic Acids Diagnostics [0.03%]
Xuhua Zhao,Yunhua Wang,Leru Wang et al.
Xuhua Zhao et al.
CRISPR/Cas12a has emerged as a powerful platform for nucleic acid diagnostics, yet its activity is widely considered to be restricted to DNA targets, limiting its applicability for direct RNA detection. Here we report a manganese-ion (Mn2+)...