Infrared spectroscopy-based chemometric modeling for monitoring mesenchymal stem cell functional decline across donors [0.03%]
基于红外光谱的化学计量模型监测供者间骨髓间充质干细胞功能衰退
Saravana Kumar Ganesan,Gargi Ghosh
Saravana Kumar Ganesan
Reliable assessment of cellular state remains a challenge in cell therapy manufacturing and regenerative medicine. Proliferative capacity, senescence, and metabolic activity are traditionally measured using labor-intensive assays, limiting ...
A biochemically structured predictive model of eukaryotic microalgal growth: Integrating radiative transfer, respiration, and pigment acclimation [0.03%]
一种预测欧菌藻生长的生化结构模型：结合辐射传输、呼吸作用和色素适应性反应
Guillaume Cogne,Claude-Gilles Dussap,Jack Legrand
Guillaume Cogne
Predictive modeling of phototrophic cultures in photobioreactors remains challenging because growth emerges from the coupling between radiative transfer, intracellular bioenergetics, and physiological acclimation. Although existing approach...
High-throughput development and scale-up of recombinant adeno-associated virus 5 anion-exchange chromatography for high-purity capsid separation [0.03%]
用于重组腺相关病毒5型衣壳分离的高通量阳离子交换层析工艺开发及放大研究
Kevin Vera,Ivan Gajic,Praveen Kusumanchi et al.
Kevin Vera et al.
Recombinant adeno-associated virus (rAAV) production commonly yields a high proportion of empty and partially filled capsids, necessitating downstream purification to enrich full capsids. Among purification approaches, anion-exchange chroma...
Screening of bispecific antibodies during single cell cloning of recombinant Chinese hamster ovary cell lines to improve titer and product quality [0.03%]
通过单克隆筛选重组中国仓鼠卵巢细胞系以提高双特异抗体滴度和产品质量
Molly Robinson,Jessica Willmott,Holly Corrigall et al.
Molly Robinson et al.
Development of therapeutic bispecific antibodies (BsAbs) poses significant manufacturability challenges associated with correct chain pairing and an increased diversity of impurities. Here we address this with a product quality assessment d...
Scalable catalyst production process for oleate hydratase whole-cell biocatalysis [0.03%]
油酸水合酶全细胞生物催化剂的可放大全细胞催化剂生产过程
Rebekka Horstmann,Mario Beckers,Jörn Viell et al.
Rebekka Horstmann et al.
The sustainable production of hydroxy fatty acids, such as 10-hydroxystearic acid (10-HSA), by biocatalysis is a promising alternative to petrochemical and castor oil-derived products. However, industrial implementation still requires an ef...
A 3D-printed multi-well lung-on-chip platform with bioprinted stromal compartment for upper airway tissue culture and antiviral drug evaluation [0.03%]
一种用于上呼吸道组织培养和抗病毒药物评价的生物打印共培养系统的三维多孔肺芯片平台
Pooja Sawant,Gudepalya Renukaiah Rudramurthy,Varun Kailaje et al.
Pooja Sawant et al.
Physiologically relevant lung-on-chip (LoC) systems remain constrained by polydimethylsiloxane (PDMS) limitations including small-molecule adsorption and mechanical deformation of device microchannels under perfusion, single-channel archite...
Targeting Warburg effect in Chinese hamster ovary cell culture with pyruvate dehydrogenase kinase inhibitors [0.03%]
丙酮酸脱氢酶激酶抑制剂在卵巢仓鼠肾细胞培养中针对Warburg效应的作用
Geetanjali Basavaraj Hubli,Richa Katiyar,Vrinda Kapoor et al.
Geetanjali Basavaraj Hubli et al.
Production of monoclonal antibodies (mAbs) in Chinese hamster ovary (CHO) cells is affected by the "Warburg effect" which is characterized by high lactate accumulation. Therefore, the present study explores the use of pyruvate dehydrogenase...
Deep dive into drug substance HCP patterns for comprehensive safety assessment in recombinant mAb formats [0.03%]
基于重组抗体药物的深入医师处方模式探究及全面安全性评估
Daniel M Waldera-Lupa,Anne Gehrke-Oltmanns,Alireza Dehghani et al.
Daniel M Waldera-Lupa et al.
The presence of host cell proteins (HCPs) as process-related impurities in monoclonal antibody (mAb) therapeutics presents risks, including immunogenicity, product instability, and reduced therapeutic efficacy. Therefore, HCP levels are see...
Application of commercial ultrafiltration membranes for protein purification by high performance countercurrent membrane purification (HPCMP) [0.03%]
商用超滤膜在高性能连续逆流超滤中的应用蛋白质分离纯化
Aylin Mohammadzadehmarandi,Nate Heller,Mohammadreza Ahmadimashhadi et al.
Aylin Mohammadzadehmarandi et al.
High performance countercurrent membrane purification (HPCMP) has emerged as a promising diffusive separation strategy for the purification of recombinant proteins and monoclonal antibodies (mAbs). However, prior studies employed dialysis m...
Demonstration of nanofiltration-based virus safety for AAV manufacturing using model viruses [0.03%]
基于纳米过滤的AAV生产病毒安全性的研究——使用模型病毒进行演示
Brian Buesing,Tomoki Igi,Daniel Strauss
Brian Buesing
Virus safety for biopharmaceutical products has been a well-established concern for several decades. While various virus reduction steps have been identified and implemented for traditional plasma-derived and monoclonal antibody products, t...