Characterization of apoptosis in fed-batch cultures highlights the impact of timely process intervention on improving recombinant protein productivity [0.03%]
表征补料分批培养中的细胞凋亡并强调及时的过程干预对提高重组蛋白产量的影响
Misaal Patel,Sri R Madabhushi
Misaal Patel
Fed-batch processes using Chinese hamster ovary (CHO) cells are primarily employed to manufacture biologics. In these cultures, cells transition from exponential growth to the stationary phase, often resulting in a viability decline. This d...
Sequential, chromosome-specific glutamine synthetase double knockout with Cas-CLOVER establishes enhanced CHO platforms for cell line development [0.03%]
用于细胞系开发的CHO平台的Cas-CLOVER逐个染色体特异性谷氨酰胺合成酶双敲除模型
Cintia Gomez Limia,Valeriya Steffey,Hsueh Che Cheng et al.
Cintia Gomez Limia et al.
Cas-CLOVER is an emerging high-fidelity genome editing system that enables precise and efficient cell engineering. In this study, we applied Cas-CLOVER to establish a robust, gene-edited platform in suspension-adapted CHO-K1 cells supportin...
Scalable desolvation synthesis, storage stability, and comprehensive biophysical characterization of human hemoglobin nanoparticles as a potential hemoglobin-based oxygen carrier [0.03%]
一种可扩展去溶剂化合成的人血红蛋白纳米颗粒的储存稳定性和全面生物物理表征，作为基于血红蛋白的潜在氧气载体
Alisyn Greenfield,Andre F Palmer
Alisyn Greenfield
Hemoglobin (Hb)-based oxygen carriers (HBOCs) are materials that exploit Hb's native oxygen transport while avoiding the adverse side-effects associated with the exposure of the cell-free, unmodified Hb tetramer (i.e. vasoconstriction, syst...
Innovative methodology for flexible monitoring of various bioprocesses by using pre-existing Raman data coupled with automated transfer learning technique [0.03%]
基于自动迁移学习技术的拉曼预处理数据灵活监测多种生物过程的新方法研究
Adèle Schini,Hadi El Radi,Johan Cailletaud et al.
Adèle Schini et al.
This study introduces an innovative approach for the flexible monitoring of bioprocesses using Raman spectroscopy coupled with automated transfer learning. Traditional Raman spectroscopy requires extensive process-specific calibration, limi...
Evaluation of gene editing in CHO cells using the Cas-CLOVER system [0.03%]
Cas-CLOVER系统在CHO细胞基因编辑评估中的应用
Tiffany McLamarrah,Efecan Aral,Michael Hoffman et al.
Tiffany McLamarrah et al.
Recent advances in gene editing technologies have transformed the genetic engineering of Chinese hamster ovary (CHO) hosts, enabling the development of cell lines with improved stability and productivity. In this study, we employed the prog...
Enhancing mRNA vaccine production: Optimization of in vitro transcription for improved yield and purity [0.03%]
mRNA疫苗生产的优化：提高体外转录产率和纯度的方法
Kaixi Zhao,Jessica Raffaele,David A Holland et al.
Kaixi Zhao et al.
In vitro transcription (IVT) is a powerful method to generate RNA which not only facilitates RNA research but also plays a key role in the development and manufacture of RNA-based vaccines. mRNA is produced via the IVT process with a DNA te...
CRISPR-Cas9 lipid nanoparticles for targeting cyclin-dependent kinases in the tumor microenvironment [0.03%]
用于肿瘤微环境细胞周期蛋白依赖性激酶基因编辑的CRISPR-Cas9脂质纳米粒
Mohammad Mashreghi,Mahere Rezazade Bazaz,Mahmoud Reza Jaafari
Mohammad Mashreghi
Due to the significant roles of cyclin-dependent kinases 4 and 6 (CDK4/6) in cancer progression, this study aimed to introduce clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9) plasmid DNA...
Enhanced lentiviral vector recovery and separation using arginine hydrochloride with CIM QA monoliths [0.03%]
使用氯化arginine和CIM QA整体柱增强慢病毒载体的回收与分离
Andrew J Kocot,Shivani Kulkarni,Ronit Ghosh et al.
Andrew J Kocot et al.
Lentiviral vectors (LVVs) offer distinct advantages including large payload capacity and stable transduction of non-dividing cells, making them well-suited for ex vivo modification of stem or immune cells. However, chromatographic purificat...
Unveiling microbial and microalgal chassis for therapeutic and diagnostic protein expression [0.03%]
用于药物和诊断蛋白质表达的微生物和微藻底盘研究
Lakshika Bhandari,Samir Kulkarni,Gunjan Prakash
Lakshika Bhandari
Biopharmaceuticals are becoming one of the most successful clinical therapeutic products for treating various disorders and are gradually being utilized across nearly all areas of medicine. They have revolutionized the treatment of numerous...
A size-exclusion chromatography fingerprinting workflow for the development of flow-through polishing operations for mAs continuous precipitation processes [0.03%]
用于mAb连续沉淀工艺流动抛光操作开发的尺寸排阻色谱指纹图谱工作流程
Mario A Gutierrez-Diaz,Scott H Altern,Todd M Przybycien et al.
Mario A Gutierrez-Diaz et al.
In this work we present a workflow for developing two-step, flow-through polishing processes for monoclonal antibodies (mAbs). The approach is demonstrated using redissolved precipitates from three CHO-derived mAbs generated by a continuous...