Photocrosslinking Activity-Based Probes to Capture the Dynamics of Ubiquitin RING E3 Ligase Interactions [0.03%]
用于捕获泛素连接酶RINGS互作动力学的光交联活性基团探针
Sarah F Chandler,Michael H Tatham,Emma Branigan et al.
Sarah F Chandler et al.
Almost all cellular processes are influenced by ubiquitination. A large family of enzymes known as E3 ligases provide the specificity for ubiquitination, with the largest class among them, the RING E3s, comprising over 600 members in humans...
Deep learning insights into β-lactamase dynamics and resistance evolution [0.03%]
深入了解β-内酰胺酶动力学和耐药性进化中的深度学习
Jing Gu,Lin Gao,Shuang Chen et al.
Jing Gu et al.
The rapid global expansion of β-lactamase-mediated antimicrobial resistance demands mechanistic approaches capable of resolving the dynamics of enzyme adaptation. Although β-lactamase evolution often involves subtle rearrangements rather ...
Roles of acidic residues in SpeG acetyltransferases-insights into importance for kinetic activity and polyamine binding in allosteric and acceptor sites [0.03%]
SpeG乙酰转移酶中酸性残基的作用——变构位点和受体位点动力学活性及多胺结合的重要性初探
Hazel N Leiva Martel,Van Thi Bich Le,Ekaterina V Filippova et al.
Hazel N Leiva Martel et al.
The Gcn5-related N-acetyltransferases (GNATs) belong to a massive superfamily of enzymes that perform a wide array of functions in different organisms. This family is comprised of smaller subfamilies, including one called the spermidine/spe...
The backside β-turn is a key structural element of Rad6-family E2 ubiquitin-conjugating enzymes [0.03%]
Rad6家族E2泛素结合酶的背面β-转角是其关键结构单元
Mahesh B Chandrasekharan,Prakash K Shukla,Andrew M Leng et al.
Mahesh B Chandrasekharan et al.
Protein ubiquitination regulates diverse cellular processes, and its dysregulation contributes to human disease, including cancer. E2 ubiquitin‑conjugating enzymes share a conserved UBC fold in which surface loops fine‑tune catalysis and ...
Arnav Jhawar,Kasey Parks,Danielle L Schmitt
Arnav Jhawar
AMP-activated protein kinase (AMPK) is a crucial regulator of cellular energy balance, affecting numerous downstream targets across various subcellular locations. Under cellular energy stress, AMPK becomes fully activated when it binds AMP ...
Correction: Characterisation of RNA guanine-7 methyltransferase (RNMT) using a small molecule approach [0.03%]
关于RNA鸟苷N7-甲基转移酶(RNMT)的小分子表征的更正通知
David W Gray,Lesley-Anne Pearson,Alain-Pierre Petit et al.
David W Gray et al.
Published Erratum
The Biochemical journal. 2026 May 6;483(5):831. DOI:10.1042/BCJ20240608_COR 2026
Target discrimination and PAM profiling of the Thermotoga maritima type I-B CRISPR system [0.03%]
Thermotoga maritima I-B型CRISPR系统的靶标识别及PAM谱分析
John Mallon,Charles J Lenihan,Sowmyaa Shridhar et al.
John Mallon et al.
Type I-B CRISPR-Cas systems represent the most abundant CRISPR subtype in nature and have emerged as powerful tools for endogenous genome editing in diverse prokaryotes. Here we reconstitute and characterize the type I-B1 system from the th...
Peeking behind the carbocation: identification of (alternative) catalytic bases in the class II active site of conifer resin acid diterpene synthases [0.03%]
窥探碳正离子背后：松香酸类二萜合成酶II类活性位点中催化碱(的鉴定)
Ahmed M A A Raslan,Reuben J Peters
Ahmed M A A Raslan
Class II diterpene cyclases (DTCs) initiate biosynthesis of the labdane-related diterpenoids (LRDs), utilizing an acid-base mechanism to catalyze bicyclization of the general diterpenoid precursor (E,E,E)-geranylgeranyl pyrophosphate (1), m...
Identification of essential residues for the biosynthesis of momilactone intermediate diterpene in wild rice Oryza officinalis [0.03%]
野生稻(Oryza officinalis)momilactone中间二萜生物合成的关键基序残基的鉴定
Yuto Hasegawa,Yoshitaka Moriwaki,Yuri Takeda-Kimura et al.
Yuto Hasegawa et al.
It is well-known that momilactones are diterpenoids that play important roles in the survival strategy of cultivated rice (Oryza sativa). Momilactones serve not only as phytoalexins against pathogens but also as allelopathic substances agai...
Structure and function of the L-MYC N-terminus impacts strategies to inhibit the MYC family of oncoproteins [0.03%]
L-MYC N末端的结构和功能影响抑制MYC致癌蛋白家族的策略
Tristan Mg Kenney,Scott Houliston,Peter Lin et al.
Tristan Mg Kenney et al.
The MYC family of transforming oncogenes function as regulators of gene transcription and is composed of three members, MYC, MYCN and MYCL. As c-MYC (MYC) is deregulated in >50% of human cancers, the role, regulation and structural features...