Hugo Schweke,Martin Pacesa,Tal Levin et al.
Hugo Schweke et al.
Protein structures are essential to understanding cellular processes in molecular detail. While advances in artificial intelligence revealed the tertiary structure of proteins at scale, their quaternary structure remains mostly unknown. We ...
The cycling and aging mouse female reproductive tract at single-cell resolution [0.03%]
单细胞分辨率下的年轻和年老小鼠雌性生殖系统
Ivana Winkler,Alexander Tolkachov,Fritjof Lammers et al.
Ivana Winkler et al.
The female reproductive tract (FRT) undergoes extensive remodeling during reproductive cycling. This recurrent remodeling and how it shapes organ-specific aging remains poorly explored. Using single-cell and spatial transcriptomics, we syst...
PARP1-DNA co-condensation drives DNA repair site assembly to prevent disjunction of broken DNA ends [0.03%]
PARP1-DNA共浓缩驱动DNA修复位点组装以防止断端DNA分离
Nagaraja Chappidi,Thomas Quail,Simon Doll et al.
Nagaraja Chappidi et al.
DNA double-strand breaks (DSBs) are repaired at DSB sites. How DSB sites assemble and how broken DNA is prevented from separating is not understood. Here we uncover that the synapsis of broken DNA is mediated by the DSB sensor protein poly(...
A synthetic differentiation circuit in Escherichia coli for suppressing mutant takeover [0.03%]
大肠杆菌中的一个合成分化电路可用于抑制突变体接管
David S Glass,Anat Bren,Elizabeth Vaisbourd et al.
David S Glass et al.
Differentiation is crucial for multicellularity. However, it is inherently susceptible to mutant cells that fail to differentiate. These mutants outcompete normal cells by excessive self-renewal. It remains unclear what mechanisms can resis...
Microglia maintain structural integrity during fetal brain morphogenesis [0.03%]
小胶质细胞维持胎儿大脑形态发生过程中的结构完整性
Akindé René Lawrence,Alice Canzi,Cécile Bridlance et al.
Akindé René Lawrence et al.
Microglia (MG), the brain-resident macrophages, play major roles in health and disease via a diversity of cellular states. While embryonic MG display a large heterogeneity of cellular distribution and transcriptomic states, their functions ...
Dainan Yu,Haifeng Wan,Chao Tong et al.
Dainan Yu et al.
Pregnancy induces dramatic metabolic changes in females; yet, the intricacies of this metabolic reprogramming remain poorly understood, especially in primates. Using cynomolgus monkeys, we constructed a comprehensive multi-tissue metabolome...
Diana R Dou,Yanding Zhao,Julia A Belk et al.
Diana R Dou et al.
Autoimmune diseases disproportionately affect females more than males. The XX sex chromosome complement is strongly associated with susceptibility to autoimmunity. Xist long non-coding RNA (lncRNA) is expressed only in females to randomly i...
Susu Chen,Yi Liu,Ziyue Aiden Wang et al.
Susu Chen et al.
Behavior relies on activity in structured neural circuits that are distributed across the brain, but most experiments probe neurons in a single area at a time. Using multiple Neuropixels probes, we recorded from multi-regional loops connect...
Integrating cellular electron microscopy with multimodal data to explore biology across space and time [0.03%]
整合细胞电子显微镜与多模式数据以探索时空生物学
Caitlyn L McCafferty,Sven Klumpe,Rommie E Amaro et al.
Caitlyn L McCafferty et al.
Biology spans a continuum of length and time scales. Individual experimental methods only glimpse discrete pieces of this spectrum but can be combined to construct a more holistic view. In this Review, we detail the latest advancements in v...
Martin Beck,Roberto Covino,Inga Hänelt et al.
Martin Beck et al.
Determining the structure and mechanisms of all individual functional modules of cells at high molecular detail has often been seen as equal to understanding how cells work. Recent technical advances have led to a flush of high-resolution s...