Deep mutational scanning of hepatitis B virus reveals a mechanism for cis-preferential reverse transcription [0.03%]
深度诱变扫描揭示乙肝病毒反向转录的顺式偏好机制
Yingpu Yu,Maximilian A Kass,Mengyin Zhang et al.
Yingpu Yu et al.
Hepatitis B virus (HBV) is a small double-stranded DNA virus that chronically infects 296 million people. Over half of its compact genome encodes proteins in two overlapping reading frames, and during evolution, multiple selective pressures...
Circadian tumor infiltration and function of CD8+ T cells dictate immunotherapy efficacy [0.03%]
昼夜节律调节肿瘤浸润CTL的募集及其疗效评估时的功能状态
Chen Wang,Qun Zeng,Zeynep Melis Gül et al.
Chen Wang et al.
The quality and quantity of tumor-infiltrating lymphocytes, particularly CD8+ T cells, are important parameters for the control of tumor growth and response to immunotherapy. Here, we show in murine and human cancers that these parameters e...
Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging [0.03%]
利用单细胞转录组和蛋白质组成像绘制人体骨髓生态位的细胞生物地理图谱
Shovik Bandyopadhyay,Michael P Duffy,Kyung Jin Ahn et al.
Shovik Bandyopadhyay et al.
Non-hematopoietic cells are essential contributors to hematopoiesis. However, heterogeneity and spatial organization of these cells in human bone marrow remain largely uncharacterized. We used single-cell RNA sequencing (scRNA-seq) to profi...
FLT3L governs the development of partially overlapping hematopoietic lineages in humans and mice [0.03%]
FLT3L调控人类和小鼠部分重叠的造血谱系的发展
Mana Momenilandi,Romain Lévy,Steicy Sobrino et al.
Mana Momenilandi et al.
FMS-related tyrosine kinase 3 ligand (FLT3L), encoded by FLT3LG, is a hematopoietic factor essential for the development of natural killer (NK) cells, B cells, and dendritic cells (DCs) in mice. We describe three humans homozygous for a los...
Small-molecule-induced epigenetic rejuvenation promotes SREBP condensation and overcomes barriers to CNS myelin regeneration [0.03%]
小分子诱导的表观遗传年轻化促进SREBP凝聚并克服中枢神经系统髓鞘再生障碍
Xuezhao Liu,Dazhuan Eric Xin,Xiaowen Zhong et al.
Xuezhao Liu et al.
Remyelination failure in diseases like multiple sclerosis (MS) was thought to involve suppressed maturation of oligodendrocyte precursors; however, oligodendrocytes are present in MS lesions yet lack myelin production. We found that oligode...
RNA aggregates harness the danger response for potent cancer immunotherapy [0.03%]
利用危险信号响应机制的RNA聚集物强效癌症免疫疗法
Hector R Mendez-Gomez,Anna DeVries,Paul Castillo et al.
Hector R Mendez-Gomez et al.
Cancer immunotherapy remains limited by poor antigenicity and a regulatory tumor microenvironment (TME). Here, we create "onion-like" multi-lamellar RNA lipid particle aggregates (LPAs) to substantially enhance the payload packaging and imm...
Julia Joung,Sai Ma,Tristan Tay et al.
Julia Joung et al.
Published Erratum
Cell. 2024 Jun 6;187(12):3161. DOI:10.1016/j.cell.2024.04.038 2024
Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB [0.03%]
正向选择CRISPR筛选发现了一个糖基转移酶中的可药用口袋，该转移酶对NF-κB的炎性信号传导是必需的
Benjamin L Lampson,Ana S Ramίrez,Marta Baro et al.
Benjamin L Lampson et al.
Nuclear factor κB (NF-κB) plays roles in various diseases. Many inflammatory signals, such as circulating lipopolysaccharides (LPSs), activate NF-κB via specific receptors. Using whole-genome CRISPR-Cas9 screens of LPS-treated cells that...
Benjamin T Throesch,Muhammad Khadeesh Bin Imtiaz,Rodrigo Muñoz-Castañeda et al.
Benjamin T Throesch et al.
A central question for regenerative neuroscience is whether synthetic neural circuits, such as those built from two species, can function in an intact brain. Here, we apply blastocyst complementation to selectively build and test interspeci...
Jia Huang,Bingbing He,Xiali Yang et al.
Jia Huang et al.
Interspecies blastocyst complementation (IBC) provides a unique platform to study development and holds the potential to overcome worldwide organ shortages. Despite recent successes, brain tissue has not been achieved through IBC. Here, we ...