Development of binding assays for the SH2 domain of Grb7 and Grb2 using fluorescence polarization [0.03%]
用于Grb7和Grb2的SH2结构域的荧光偏振结合试验的发展
Jean-Philippe Luzy,Huixiong Chen,Brunilde Gril et al.
Jean-Philippe Luzy et al.
Adaptor proteins Grb7 and Grb2 have been implicated as being 2 potential therapeutic targets in several human cancers, especially those that overexpress ErbB2. These 2 proteins contain both a SH2 domain (Src homology 2) that binds to phosph...
Comparative Study
Journal of biomolecular screening. 2008 Feb;13(2):112-9. DOI:10.1177/1087057107312124 2008
Identification of glucose-dependant insulin secretion targets in pancreatic beta cells by combining defined-mechanism compound library screening and siRNA gene silencing [0.03%]
通过结合明确机制化合物筛选和siRNA干扰技术在胰岛β细胞中鉴定葡萄糖依赖性胰岛素分泌靶点
Weizhen Wu,Jin Shang,Yue Feng et al.
Weizhen Wu et al.
Identification and validation of novel drug targets continues to be a major bottleneck in drug development, particularly for polygenic complex diseases such as type 2 diabetes. Here, the authors describe an approach that allows researchers ...
An efficient and fully automated high-throughput transfection method for genome-scale siRNA screens [0.03%]
一种高效的全自动化高通量转染方法用于基因组规模的siRNA筛选
Namjin Chung,Louis Locco,Kevin W Huff et al.
Namjin Chung et al.
RNA interference (RNAi), combined with the availability of genome sequences, provides an unprecedented opportunity for the massive and parallel investigations of gene function. Small interfering RNA (siRNA) represents a popular and quick ap...
Quantitative high-throughput screening using a live-cell cAMP assay identifies small-molecule agonists of the TSH receptor [0.03%]
利用活细胞cAMP检测的定量高通量筛选鉴定出TSH受体的小分子激动剂
Steve Titus,Susanne Neumann,Wei Zheng et al.
Steve Titus et al.
The thyroid-stimulating hormone (TSH; thyrotropin) receptor belongs to the glycoprotein hormone receptor subfamily of 7-transmembrane spanning receptors. TSH receptor (TSHR) is expressed mainly in thyroid follicular cells and is activated b...
Quantitative assessment of hit detection and confirmation in single and duplicate high-throughput screenings [0.03%]
高通量单次和重复筛选中活性化合物识别与确证的定量评估研究
Zhijin Wu,Dongmei Liu,Yunxia Sui
Zhijin Wu
The process of identifying active targets (hits) in high-throughput screening (HTS) usually involves 2 steps: first, removing or adjusting for systematic variation in the measurement process so that extreme values represent strong biologica...
Growth, drug susceptibility, and gene expression profiling of Plasmodium falciparum cultured in medium supplemented with human serum or lipid-rich bovine serum albumin [corrected] [0.03%]
补加人血清或富含脂质的牛血清白蛋白培养基对恶性疟原虫生长、药物敏感性及基因表达的影响*[校正刊出]
Kshipra Singh,Ameeta Agarwal,Shabana I Khan et al.
Kshipra Singh et al.
In vitro cultivation of Plasmodium falciparum has been extremely useful in understanding the biology of the human malaria parasite as well as research on the discovery of new antimalarial drugs and vaccines. A chemically defined serum-free ...
Differential assay for high-throughput screening of antibacterial compounds [0.03%]
用于抗菌化合物高通量筛选的差异分析法
Shaun P Falk,Andrew T Ulijasz,Bernard Weisblum
Shaun P Falk
The previously described Bacillus subtilis reporter strain BAU-102 is capable of detecting cell wall synthesis inhibitors that act at all stages of the cell wall synthesis pathway. In addition, this strain is capable of detecting compounds ...
A flow-through fluorescence polarization detection system for measuring GPCR-mediated modulation of cAMP production [0.03%]
一种流动注射型荧光偏振检测系统用于测量G蛋白偶联受体介导的环磷酸腺苷生成调节效应
Jeroen Kool,André van Marle,Saskia Hulscher et al.
Jeroen Kool et al.
A flow-through fluorescence polarization (FP) detection system that makes use of a novel high-performance liquid chromatography (HPLC) fluorescence detector modified with polarization filters was developed. This flow-through FP detection sy...
Application of the BD ACTOne technology for the high-throughput screening of Gs-coupled receptor antagonists [0.03%]
BD ACTONE技术在Gs偶联受体拮抗剂高通量筛选中的应用研究
András Visegrády,András Boros,Zsolt Némethy et al.
András Visegrády et al.
A novel technology for monitoring the changes of 3,'5'-adenosine cyclic monophosphate (cAMP) in live cells suitable for drug screening relies on the use of cyclic nucleotide-gated channels as biosensors coexpressed with the appropriate targ...
Validation of a medium-throughput electrophysiological assay for KCNQ2/3 channel enhancers using IonWorks HT [0.03%]
一种中通量的电生理检测试剂盒用于KCNQ2/3通道增强子离子流筛选离子工作站高通量筛选验证
Flora Jow,Ru Shen,Pranab Chanda et al.
Flora Jow et al.
Enhancers of KCNQ channels are known to be effective in chronic pain models. To discover novel enhancers of KCNQ channels, the authors developed a medium-throughput electrophysiological assay by using the IonWorks platform. Screening of 20 ...