Plate-based diversity selection based on empirical HTS data to enhance the number of hits and their chemical diversity [0.03%]
基于经验HTS数据的板式多样性选择以增加命中率和化学多样性
Sai Chetan K Sukuru,Jeremy L Jenkins,Rohan E J Beckwith et al.
Sai Chetan K Sukuru et al.
Typically, screening collections of pharmaceutical companies contain more than a million compounds today. However, for certain high-throughput screening (HTS) campaigns, constraints posed by the assay throughput and/or the reagent costs mak...
Development and evaluation of a FACS-based medium-throughput assay for HCV entry inhibitors [0.03%]
一种基于FACS的中通量测定法的建立及用于评估HCV入胞抑制剂效力
Sigrid Ziegler,Bernd Kronenberger,Beatrice A-M Albrecht et al.
Sigrid Ziegler et al.
The interaction between the hepatitis C virus (HCV) envelope glycoprotein E2 and the human tetraspanin protein CD81 is one of the key events involved in HCV cell entry. Therefore, compounds that interfere with this interaction may be useful...
Overcoming problems of compound storage in DMSO: solvent and process alternatives [0.03%]
克服DMSO中化合物储存的问题：溶剂及工艺替代方法
Timothy J Waybright,John R Britt,Thomas G McCloud
Timothy J Waybright
The common practice of preparing storage libraries of compounds in 100% DMSO solution well in advance of bioassay brings with it difficulties that affect the accuracy of the data obtained. This publication presents a series of studies done ...
A simplified scintillation proximity assay for fatty acid synthase activity: development and comparison with other FAS activity assays [0.03%]
一种简化的心磷脂合成酶活性闪烁接近法测定及其与其他心磷脂合成酶活性测定方法的比较
Nathan W Bays,Armetta D Hill,Ilona Kariv
Nathan W Bays
Fatty acid synthase (FAS), an essential enzyme for de novo lipogenesis, has been implicated in a number of disease states, including obesity, dyslipidemia, and cancer. To identify small-molecule inhibitors of FAS, the authors developed a be...
Comparative Study
Journal of biomolecular screening. 2009 Jul;14(6):636-42. DOI:10.1177/1087057109335746 2009
Can phage display be used as a tool to functionally identify endogenous eat-me signals in phagocytosis? [0.03%]
噬菌体展示能否作为工具来识别吞噬作用中内源性的eat-me信号？
Nora B Caberoy,Yixiong Zhou,Wei Li
Nora B Caberoy
Removal of apoptotic cells and cellular debris by phagocytosis is essential for development, tissue homeostasis, and resolution of inflammation. Eat-me signals control the initiation of phagocytosis, holding a key to the understanding of ph...
Polyplexed flow cytometry protein interaction assay: a novel high-throughput screening paradigm for RGS protein inhibitors [0.03%]
用于RGS蛋白质抑制剂的新型高通量筛选模式的多聚体流式细胞术蛋白质相互作用测定法
David L Roman,Shodai Ota,Richard R Neubig
David L Roman
Intracellular signaling cascades are a series of regulated protein-protein interactions that may provide a number of targets for potential drug discovery. Here, the authors examine the interaction of regulators of G-protein signaling (RGS) ...
Development of a fluorescence-based, ultra high-throughput screening platform for nanoliter-scale cytochrome p450 microarrays [0.03%]
一种用于纳升规模细胞色素P450微阵列的超高通量筛选平台的开发（英汉对照）
Sumitra M Sukumaran,Benjamin Potsaid,Moo-Yeal Lee et al.
Sumitra M Sukumaran et al.
Cytochrome P450 enzyme (CYP450s) assays are critical enzymes in early-stage lead discovery and optimization in drug development. Currently available fluorescence-based reaction assays provide a rapid and reliable method for monitoring CYP45...
Primary leukocyte screens for innate immune agonists [0.03%]
primary leukocyte screens for innate immune agonists Innate免疫激动剂的一线白细胞筛选方法
Amber Goodchild,Nicole Nopper,Alexis Craddock et al.
Amber Goodchild et al.
The innate immune system of mammals is a key defense mechanism against invading foreign pathogens. Innate immune stimulants may have applications as vaccine adjuvants as well as in the treatment of cancer and some viral diseases, and clinic...
Novel temperature activation cell-based assay on thermo-TRP ion channels [0.03%]
新型温度活化电池基于热敏瞬时受体电位离子通道的检测方法研究
Eduardo Aneiros,Michael Dabrowski
Eduardo Aneiros
The precise temperature control of the ABI Prism 7900HT Sequence Detection System designed for detection of fluorescence of a biological sample in real-time PCR assays (TaqMan assays) was used to activate Thermo-TRP ion channels, enabling a...
Dequalinium, a new inhibitor of Mycobacterium tuberculosis mycothiol ligase identified by high-throughput screening [0.03%]
一种新的抑制结核杆菌麦洛硫酯酶的化合物Dequalinium的高通量筛选及鉴定研究
Maria-Teresa Gutierrez-Lugo,Heather Baker,Joseph Shiloach et al.
Maria-Teresa Gutierrez-Lugo et al.
Mycothiol ligase (MshC) is a key enzyme in the biosynthesis of mycothiol, a small molecular weight thiol that is unique to actinomycetes and whose primary role is to maintain intracellular redox balance and remove toxins. MshC catalyzes the...