Cystic Fibrosis Modulator Therapies: Bridging Insights from CF to other Membrane Protein Misfolding Diseases [0.03%]
囊性纤维化调理疗法：从囊性纤维化到其他膜蛋白错误折叠疾病的见解桥梁
Minsoo Kim,Lars Plate
Minsoo Kim
Cystic Fibrosis (CF) is a genetic disorder resulting from mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene, leading to a faulty CFTR protein. Dysfunctional CFTR causes chloride ion imbalance, resulting in den...
Crystal Structures of Diaryl Hydrazone and Sulfone Stabilizers in Complex with an Amyloidogenic Light Chain Reveal an Alternate Ligand-Binding Cavity [0.03%]
二芳基腙和磺胺稳定剂与淀粉样原轻链的晶体结构揭示了一个替代配体结合腔
Nicholas L Yan,Ian A Wilson,Jeffery W Kelly
Nicholas L Yan
Stabilization of amyloidogenic immunoglobulin light chains (LCs) by binding of small molecule "kinetic stabilizers" is under development as a novel treatment for light chain amyloidosis. From a high-throughput screen, we previously identifi...
Strategies for Competitive Activity-Based Protein Profiling in Small Molecule Inhibitor Discovery and Characterization [0.03%]
基于竞争的蛋白质谱学策略在小分子抑制剂发现和表征中的应用
He Zhu,Mona Sharafi,Wei Pin Teh et al.
He Zhu et al.
Since its introduction by Cravatt and colleagues in 1999, activity-based protein profiling (ABPP) has become widely utilized throughout academia, government, and industry laboratories to study enzymes spanning numerous gene families in a mu...
Ruitu Lyu,Kinga Pajdzik,Hui-Lung Sun et al.
Ruitu Lyu et al.
5-Formylcytosine (f5C) modification is present in human mitochondrial methionine tRNA (mt-tRNAMet) and cytosolic leucine tRNA (ct-tRNALeu), with their formation mediated by NSUN3 and ALKBH1. f5C has also been detected in mRNA of yeast and h...
Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation [0.03%]
药物靶向PDIA1抑制NLRP3炎性小体组装和活化
Jessica D Rosarda,Caroline R Stanton,Emily B Chen et al.
Jessica D Rosarda et al.
The NLRP3 inflammasome is a cytosolic protein complex that regulates innate immune signaling in response to diverse pathogenic insults through the proteolytic processing and secretion of pro-inflammatory cytokines such as IL-1β. Hyperactiv...
Nanopore Direct RNA Sequencing for Modified Uridine Nucleotides Yields Signals Dependent on the Physical Properties of the Modified Base [0.03%]
纳米孔直接RNA测序法对修饰的尿嘧啶核苷酸产生的信号取决于修饰碱基的物理性质
Aaron M Fleming,Justin C Dingman,Yizhou Wu et al.
Aaron M Fleming et al.
Sequencing for RNA modifications with the nanopore direct RNA sequencing platform provides ionic current levels, helicase dwell times, and base call data that differentiate the modifications from the canonical form. Herein, model RNAs were ...
Protein Glycosylation Patterns Shaped By the IRE1-XBP1s Arm of the Unfolded Protein Response [0.03%]
未折叠蛋白反应的IRE1-XBP1s通路塑造蛋白糖基化模式
Kenny Chen,Matthew D Shoulders
Kenny Chen
The unfolded protein response (UPR) is a sensing and signaling pathway that surveys the endoplasmic reticulum (ER) for protein folding challenges and responds whenever issues are detected. UPR activation leads to upregulation of secretory p...
Strategies for detecting aminoacylation and aminoacyl-tRNA editing in vitro and in cells [0.03%]
用于检测体内外氨基酰化和氨基酰-tRNA编辑的策略
Rylan R Watkins,Arundhati Kavoor,Karin Musier-Forsyth
Rylan R Watkins
Aminoacyl-tRNA synthetases (aaRSs) maintain translational fidelity by ensuring the formation of correct aminoacyl-tRNA pairs. Numerous point mutations in human aaRSs have been linked to disease phenotypes. Structural studies of aaRSs from h...
Chemical and biomolecular insights into the Staphylococcus aureus agr quorum sensing system: Current progress and ongoing challenges [0.03%]
金黄色葡萄球菌agr群体感应系统的化学及生物分子研究：当前进展与未来挑战
Thomas J Polaske,Korbin H J West,Ke Zhao et al.
Thomas J Polaske et al.
Staphylococcus aureus is a ubiquitous bacterium that has become a major threat to human health due to its extensive toxin production and tremendous capacity for antibiotic resistance (e.g., MRSA "superbug" infections). Amid a worsening anti...
Yi Yang,Mingkuan Chen,Mengyao Wu et al.
Yi Yang et al.
Truncated mucin-type O-glycans, such as Tn-associated antigens, are aberrantly expressed biomarkers of cancer, but remain challenging to target. Reactive antibodies to these antigens either lack high-affinity or are prone to antigen escape....