Structural and Biochemical Characterization of Fusobacterium nucleatum Enoyl-ACP Reductase II (FabK) Reveals the Basis for Bacterial Species-Specific Inhibition [0.03%]
Kristiana Avad,Osama Alaidi,Destiny Okpomo et al.
Kristiana Avad et al.
Fusobacterium nucleatum is a Gram-negative anaerobic bacterium ubiquitous in the oral cavity and increasingly recognized for its involvement in diverse clinical conditions, including periodontal disease, inflammatory bowel disease, prematur...
Othman Al Musaimi,Daryl R Williams
Othman Al Musaimi
A significant advancement in Merrifield's classic solid-phase peptide synthesis (SPPS) that greatly expands the scope of accessible peptide structures is reported here. Building upon the one-bead, one-compound (OBOC) concept, this approach ...
Photoinduced Electron Transfer Informs on Pathway Coupling in Flavin-Based Electron Bifurcation [0.03%]
Seth A Wiley,Carolyn E Lubner
Seth A Wiley
Flavin-based electron bifurcation (FBEB) is an enzymatic mechanism that generates extremely high-energy electrons to drive unfavorable chemical reactions. It is utilized by the NADH-dependent ferredoxin:NADP+-oxidoreductase (Nfn) enzyme in ...
Optimization of Indole- and Pyrazole-fused Glycyrrhetinic Acid Derivatives as Potent PTP1B Inhibitors: In Silico, In Vitro, In Vivo, and Metabolomic Studies [0.03%]
Mitzi López-Sánchez,Hannya Mendoza-Mota,Ledy De-la-Cruz-Martínez et al.
Mitzi López-Sánchez et al.
Protein tyrosine phosphatase 1B (PTP1B) is a crucial enzyme involved in regulating insulin and leptin signaling pathways, making it a promising target for treating type 2 diabetes. In this study, we synthesized 14 derivatives of indole- and...
Jacob Kress,Sookyeong Kim,Caitlynn Bryant et al.
Jacob Kress et al.
The development of genetically encoded labels for multicolor experiments requires a diverse palette of fluorescent proteins that are well-behaved. Here, we report mSumireF, a monomeric variant of the violet fluorescent protein Sumire. On it...
Is Thiourea the Weak Link? An Investigation of the In Vitro and In Vivo Destabilization of [203Pb]- and [212Pb]Pb2+ Thiourea-Based Bioconjugates [0.03%]
Brooke L McNeil,Luke Wharton,Chao-Cheng Chen et al.
Brooke L McNeil et al.
Fibroblast activation protein (FAP) is overexpressed in a variety of cancers, making it an attractive target for bifunctional chelator-based radiopharmaceuticals. This study initially aimed to assess the effect of chelator structure on the ...
Blue Fluorescent Siloxytecans Exhibit Potent Anticancer Activity and Enable Direct Real-Time Quantification of Intracellular Uptake [0.03%]
Isabella Lo,Sanika Vaidya,Ashley Mo et al.
Isabella Lo et al.
Notwithstanding the natural abundance of silicon on Earth, silicon-containing compounds comprise relatively few pharmaceutical drugs, though several silylated natural products have demonstrated greater bioavailability and lipophilicity. Her...
Prebiotic Roles of Formaldehyde as an Activating Agent and a Building Block in Solid-State Peptide Modification [0.03%]
甲醛作为活化剂和构建模块在固相肽修饰中发挥的益生作用
Jamie C Thuan,Anuja Koirala,James Pirez et al.
Jamie C Thuan et al.
Signal transduction by gaseous small molecules is an essential process in modern living systems, where the gasotransmitters relay cellular signals to bio-(macro)-molecules through covalent bond formation. However, the origin or a primordial...
A Tutorial Review on Surface Plasmon Resonance Biosensors: Applications in Biomedicine [0.03%]
基于表面等离子体共振生物传感器的生物医学应用进展及评述
Antony Chirco,Elisabetta Meacci,Giancarlo Margheri
Antony Chirco
Surface Plasmon Resonance (SPR) has proven to be one of the most effective technologies in terms of specificity, affinity, and determination of kinetic parameters for evaluating interactions between macromolecules. The focus of this tutoria...
Photoactivatable, Biomimetic Ligand photoMultiTASQ Traps DNA/RNA G‑Quadruplexes and Their Protein Binding Partners [0.03%]
光活化生物模拟配体photoMultiTASQ捕获DNA/RNA G-四链体及其蛋白质结合伴侣
Sandy Raevens,Yehuda M Danino,Cécile Desingle et al.
Sandy Raevens et al.
The biological role of DNA and RNA G-quadruplexes (G4s) is relayed to the cellular circuitry by a plethora of proteins known as G4-binding proteins (G4PBs). It is thus critical to decipher the formation of the G4/G4BP complexes to accuratel...