Deploying a JupyterHub Server for Academic Research Using Netbooks as an Example [0.03%]
使用Netbook为例部署JupyterHub服务器以供学术研究使用
Marouen Ben Guebila,Enakshi Saha,Mia Shapoval et al.
Marouen Ben Guebila et al.
Jupyter Notebooks have transformed the way we conduct computational research thanks to a versatile platform that combines code, annotations, plots, and a lightweight format that enables versioning. However, steps for installing dependencies...
Induction of Murine Models of Autoimmune Myocarditis, Valvulitis, and Cardiomyopathy [0.03%]
自身免疫性心肌炎、瓣膜炎和心肌病的小鼠模型的诱导
Jennifer M Myers,DeLisa Fairweather,Sally A Huber et al.
Jennifer M Myers et al.
Myocarditis and valvulitis are inflammatory diseases affecting myocardium and heart valves. Myocarditis, a viral-induced disease of myocardium, may lead to dilated cardiomyopathy and loss of heart function, and valvulitis leads to deformed ...
Enzymatic Synthesis of NAD [0.03%]
酶法合成NAD+
Emily Boyle,Olivia Zhang,Yana Cen
Emily Boyle
Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite involved in numerous biological processes, functioning both as a redox cofactor in cellular energy metabolism and as a co-substrate for NAD+-utilizing enzymes, including si...
Optimized MTT Assay for Metabolic Activity Evaluation in Human Spermatozoa [0.03%]
人精子代谢活性评价的优化MTT实验方法
Bárbara Guerra-Carvalho,Eva V Gonçalves,Mário Sousa et al.
Bárbara Guerra-Carvalho et al.
The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay is low-cost, widely available, and mainly used to assess cell viability as a function of cellular oxidoreductase enzyme-dependent conversion of the water-soluble t...
Assessment of Doxorubicin Internalization and Cytotoxicity in Primary Tumor Spheroids After Collagen Digestion [0.03%]
消化胶原蛋白后评估多柔比星在原发肿瘤类器官球体中的内吞作用及细胞毒效应
Alessandra Lo Cicero,Gabriele Lo Buglio,Simona Campora et al.
Alessandra Lo Cicero et al.
The limited penetration of chemotherapeutic agents into solid tumors remains a major obstacle to effective cancer treatment and is strongly influenced by the extracellular matrix (ECM). Three-dimensional (3D) tumor spheroids derived from pr...
Ta&#x;Aliyah M Jones,Stephen Hurst,Michael J Bround
Ta&#x;Aliyah M Jones
Mitochondrial calcium (mCa2+) homeostasis promotes oxidative metabolism within the physiological range; however, dysregulation can trigger necrotic cell death in diseases such as cardiac ischemia-reperfusion injury, muscular dystrophy, and ...
Liquid-Phase Synthesis of DNA Aptamer-Based PROTACs Using the Postsynthesis Conjugation Method [0.03%]
基于 postsynthesis 偶联法的 DNA 适配体基液相PROTAC合成法
Hitomi Terauchi,Genichiro Tsuji,Yosuke Demizu
Hitomi Terauchi
Proteolysis-targeting chimeras (PROTACs) have emerged as a promising strategy for the selective degradation of intracellular proteins by harnessing the ubiquitin-proteasome system. However, the development of PROTACs is often limited by the...
Sequence-Specific Primer Polymerase Chain Reaction Genotyping of Interleukin-22 Single Nucleotide Polymorphisms rs2227473 and rs2227513 with Validation by Sanger Sequencing [0.03%]
基于Sanger测序验证的白细胞介素-22基因单核苷酸多态性位点rs2227473和rs2227513的序列特异性引物聚合酶链锁反应基因分型研究
Julyane Schavaren,Matheus Braga,Aléia Harumi Uchibaba Yamanaka et al.
Julyane Schavaren et al.
Single nucleotide polymorphisms (SNPs) can modify protein expression or function, influencing susceptibility to various diseases. Interleukin-22 (IL-22), involved in epithelial barrier homeostasis and tissue repair, has shown complex roles ...
Sequence-Specific Primer Polymerase Chain Reaction Genotyping of the Human TLR9 Promoter Polymorphism rs187084 (-1486T>C) [0.03%]
人类TLR9启动子多态位点rs187084(-1486T>C)的序列特异性引物PCR基因分型法
Guilherme Castellani,Fernanda Pelisson Massi,Aléia Harumi Uchibaba Yamanaka et al.
Guilherme Castellani et al.
This article describes a validated and optimized sequence-specific primer polymerase chain reaction (PCR-SSP) protocol for genotyping the human TLR9 promoter polymorphism rs187084 (-1486T>C). Promoter single nucleotide polymorphisms (SNPs) ...
Identifying Donor Immune Cells in the Intestinal Mucosa of Chimeric Recipient Mice by Immunofluorescence [0.03%]
免疫荧光鉴定供者免疫细胞在嵌合受体小鼠肠道黏膜中的分布
Vinicius Canale,Samuel Asiedu,Fuad Malki et al.
Vinicius Canale et al.
Murine competitive congenic bone marrow transplantation is an important method used to identify genetic contributions to immune cell abundance, activation, differentiation, and mobilization, either in the bone marrow or in the peripheral or...