High-Throughput Isolation of Nanomouse-Derived VHH Domains: A Practical Guide from Immunization to Nanobody Expression [0.03%]
纳米尔兔VHH域的高通量分离：从免疫到纳米体表达的实用指南
Tessa J Casselman,Asa W Huffaker,Kristie C Mitchell et al.
Tessa J Casselman et al.
Nanobodies are small but specific heavy chain-only antibody fragments. Their small size, relative stability, and ability to access difficult to reach deep-tissue antigens makes them valuable research, diagnostic, and therapeutic tools. Nano...
Generation of Biparental Bovine Embryos via Haploid Cell Injection (Semi-cloning) for Embryo Production with Predetermined Parental Genomes [0.03%]
基于半克隆技术的父母本基因组可测定牛胚胎生产方法及应用
Luis Aguila,Jacinthe Therrien,Lawrence C Smith
Luis Aguila
This protocol describes a method for generating biparental bovine embryos with predetermined paternal genomes using haploid androgenetic blastomere injection (semi-cloning). The approach enables replacement of sperm at fertilization with pr...
A Randomized Double-Blind Placebo-Controlled Multicentric Clinical Trial of an Ayurvedic Intervention, Varadi Kwatha Ghana Vati, as an Adjunct vs Treatment as Usual in the Management of Type-2 Diabetes Mellitus: A Study Protocol [0.03%]
一种印度传统医学药物Varadi Kwatha Ghana Vati辅助治疗2型糖尿病 mellitus的随机双盲安慰剂对照多中心临床试验研究方案
Suhas Chaudhary,Jeuti Rani Das,Kishor Gavali et al.
Suhas Chaudhary et al.
Background: Diabetes mellitus is a major and growing contributor to morbidity and mortality. Indian Council of Medical Research-India Diabetes study reports a prevalence of 11.4% for diabetes and 15.3% for prediabetes in ...
Impact of Hypertrophy-Oriented Resistance Training on Muscle Mass and Quality of Life in Patients with Unresectable Pancreatic Cancer (HY-PANC): Study Protocol for a Randomized Controlled Trial [0.03%]
针对不可切除性胰腺癌患者的以增肌为目的的抗阻运动对其肌肉质量和生活质量影响的研究方案：一项随机对照试验（HY-PANC）
Pablo Braceras Arias,Silvia Burgos Postigo,Lidia B Alejo
Pablo Braceras Arias
Background: Unresectable pancreatic cancer (PC) is characterized by a high prevalence of sarcopenia and cachexia, which negatively impact survival, treatment toxicity, and quality of life (QoL). Currently, evidence evalua...
NaP-TRAP: A Versatile and Accessible Workflow to Dissect Principles of Translational Regulation and mRNA Stability [0.03%]
NaP-TRAP：一种分解翻译调节和mRNA稳定性的通用且易于访问的工作流程
Amit Gupta,Anna Z Struba,Srihari Madhavan et al.
Amit Gupta et al.
The translation of messenger RNA (mRNA) into protein is tightly regulated by both cellular trans-factors and cis-regulatory elements encoded within transcripts. Although transcript fate can be measured by transcript abundance or translation...
High-Throughput Focal Adhesion Analysis Software for Automated Detection, Tracking, and Classification of Cell-Matrix Adhesions [0.03%]
高通量粘着斑分析软件：自动检测、跟踪和分类细胞基质粘附点
Majid Rostami,Sangyoon J Han
Majid Rostami
Focal adhesions are dynamic multi-protein complexes that mechanically link the actin cytoskeleton to the extracellular matrix through integrin receptors. A single migrating cell can contain >1000 adhesion structures simultaneously, exhibiti...
Beyond Fear Learning in Isolation: Integration of Social Dimensions into Aversive Learning [0.03%]
超越孤立的恐惧学习：将社会维度整合到厌恶性学习中
Maria M Diehl
Maria M Diehl
Aversive learning paradigms in laboratory settings have led to valuable decades of research on the acquisition and expression of memories that produce fear and avoidance. However, the majority of these studies have focused on situations in ...
An Optimized and Improved Protocol for Efficient Isolation of Extracellular RNA from Human Serum [0.03%]
一种高效分离人血清外泌体RNA的方法优化与改进
Rahul Patel,Namita Mishra,Rasna Gupta et al.
Rahul Patel et al.
The extraction of extracellular RNA from human serum is challenging due to its low abundance, high degree of fragmentation, and susceptibility to rapid degradation. These limitations are further compounded in pediatric research, where low b...
A Biomedical Researcher's Guide for Analyzing Short Tandem Repeat (STR) Genotypes of Human Cell Lines and in Vitro Tissue Samples Using Three Standard Authentication Algorithms [0.03%]
人细胞系和体外组织样本的短串联重复序列基因型分析标准鉴别算法指南
Christopher T Korch
Christopher T Korch
Cell line authenticity is critical for producing reproducible and rigorously valid biomedical research. As of version 14 of its Register of Misidentified Cell Lines, released in 2026, the International Cell Line Authentication Committee (IC...
Application of Microbial and Parasitology Techniques for Diagnosis in Laboratory Rodents [0.03%]
微生物及寄生虫技术在实验动物诊断中的应用
Debora Rapaport,Maya Levin Arama,Michael Harlev
Debora Rapaport
Pathogen diagnosis in rodents through effective health monitoring programs is essential for maintaining high welfare and standards in laboratory animal facilities. Although many animal facilities are increasingly adopting real-time polymera...