Joseph D Hurley,Andrew C Kruse
Joseph D Hurley
Protein-peptide interactions are central to many biological processes and form the basis for many techniques in basic research and therapeutic discovery. A common research challenge is to identify specific peptide ligands or binding motifs ...
2'-O-Acetal Levulinic Ester Ribonucleoside 3'-Phosphoramidites for the Solid-Phase Synthesis of Long RNA [0.03%]
用于长RNA固相合成的2'-O-乙缩醛富马酯核糖核苷3'-磷酰胺
Zidi Lyu,Adam Katolik,Masad J Damha
Zidi Lyu
Solid-phase RNA synthesis is challenging because the 2'-hydroxyl requires a protecting group that is stable during chain assembly yet easily removable without degrading the product. Driven by the demand for long RNA therapeutics, we report ...
Protein Extraction From Mycobacterium spp. for Identification by MALDI-TOF Mass Spectrometry [0.03%]
基于基质辅助激光解吸电离飞行时间质谱鉴定的分枝杆菌蛋白质提取方法研究
Lucas Evangelista Marques,Stephanie Chrystine Balestro Mota,Christoffel Opperman et al.
Lucas Evangelista Marques et al.
The identification of mycobacteria remains challenging due to the high species diversity within the genus and the lack of a molecular method able to identify them in a single assay. Matrix-assisted laser desorption/ionization time-of-flight...
Correction: Impact of Hypertrophy-Oriented Resistance Training on Muscle Mass and Quality of Life in Patients with Unresectable Pancreatic Cancer (HY-PANC): Study Protocol for a Randomized Controlled Trial [0.03%]
hypertrophy- oriented抗阻训练对不可切除胰腺癌患者肌肉质量及生活质量影响的随机对照试验方案_correction_ 研究方案
Pablo Braceras Arias,Silvia Burgos Postigo,Lidia B Alejo
Pablo Braceras Arias
Published Erratum
Current protocols. 2026 Jul;6(7):e70441. DOI:10.1002/cpz1.70441 2026
An Integrated Field Protocol for Multi-taxon Biodiversity Surveys in African Rangeland Ecosystems [0.03%]
非洲草原生态系统多分类群生物多样性调查的综合野外方法学protocol
Edward Jenkins,Mael Glon,Julia Gulka et al.
Edward Jenkins et al.
Rangelands directly support both human livelihoods and wildlife, yet they are increasingly threatened by degradation, urban and agricultural expansion, and a changing climate. Healthy rangelands sequester carbon in soils, helping to mitigat...
Conditioned Medium from Irradiated Keratinocytes as a Tool for Investigating Communication within the Melano-epidermal Unit [0.03%]
辐照角质细胞的条件培养基作为研究黑素单位内通讯的工具
Lais Zortéa,Larissa Gomes Gusmão de Almeida,Daza de Moraes Vaz Batista Filgueira et al.
Lais Zortéa et al.
Keratinocytes and melanocytes form the melano-epidermal unit, whose communication is essential for maintaining the integrity, homeostasis, and functionality of the skin. This interaction is mediated by keratinocytes' release of various solu...
Weight Measurement [0.03%]
体重测量
Andrew Hurdle,Michael Guzy,Sean Gallagher
Andrew Hurdle
This unit provides information to aid in the selection and proper use of a laboratory balance. Laboratory balances are used for measuring the mass of an object and come in two main types: mechanical and electronic. Electronic balances gener...
Enrichment and Characterization Protocol for Plant-Derived Nanovesicles from the Aquatic Plant Wolffia globosa [0.03%]
Wolffia globosa 水生植物衍生纳米囊泡的富集和表征方案
Arunya Jiraviriyakul,Manatsanan Piamano,Korawut Pimpa et al.
Arunya Jiraviriyakul et al.
This article presents a rigorous and reproducible protocol for the enrichment of plant-derived nanovesicles (PDNVs) from the aquatic plant Wolffia globosa using differential ultracentrifugation combined with sucrose density gradient enrichm...
High-Throughput Isolation of Nanomouse-Derived VHH Domains: A Practical Guide from Immunization to Nanobody Expression [0.03%]
纳米尔兔VHH域的高通量分离：从免疫到纳米体表达的实用指南
Tessa J Casselman,Asa W Huffaker,Kristie C Mitchell et al.
Tessa J Casselman et al.
Nanobodies are small but specific heavy chain-only antibody fragments. Their small size, relative stability, and ability to access difficult to reach deep-tissue antigens makes them valuable research, diagnostic, and therapeutic tools. Nano...
Generation of Biparental Bovine Embryos via Haploid Cell Injection (Semi-cloning) for Embryo Production with Predetermined Parental Genomes [0.03%]
基于半克隆技术的父母本基因组可测定牛胚胎生产方法及应用
Luis Aguila,Jacinthe Therrien,Lawrence C Smith
Luis Aguila
This protocol describes a method for generating biparental bovine embryos with predetermined paternal genomes using haploid androgenetic blastomere injection (semi-cloning). The approach enables replacement of sperm at fertilization with pr...