Generation of Biparental Bovine Embryos via Haploid Cell Injection (Semi-cloning) for Embryo Production with Predetermined Parental Genomes
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This protocol describes a method for generating biparental bovine embryos with predetermined paternal genomes using haploid androgenetic blastomere injection (semi-cloning). The approach enables replacement of sperm at fertilization with pre-genotyped haploid ... ...
