Efficient generation of human cDC1-like cells to detect and enhance tumor-reactive T cells via CD4+ T cell help [0.03%]
高效生成人类cDC1样细胞以检测和增强肿瘤反应性T细胞的CD4+T细胞帮助
Yizhi Wang,Xin Lei,Tom de Wit et al.
Yizhi Wang et al.
Classical type 1 dendritic cells (cDC1s) are crucial to anti-tumor immunity by cross-presenting tumor antigens and priming cytotoxic CD8+ T lymphocytes (CTLs). Licensing via CD4+ T cell help endows cDC1s with enhanced cross-presentation and...
HIT-MAP: A scalable approach to multimodal mapping of subcellular organization [0.03%]
HIT-MAP：亚细胞组织多模态图谱的可拓展构建方法
Gege Qian,Joonwon Kim,Richa Tiwari et al.
Gege Qian et al.
Multimodal mapping of subcellular protein organization through imaging and interaction proteomics has been mostly confined to large consortia, owing to high per-target costs, reliance on target-specific antibodies or libraries of epitope-ta...
Subtype-resolved norepinephrine imaging with an engineered α1D-adrenergic receptor indicator [0.03%]
工程型α1D肾上腺素受体示踪剂的去甲肾上腺素亚型分辨成像
Valentin Lu Rohner,Zacharoula Kagiampaki,Pauline Bohne et al.
Valentin Lu Rohner et al.
Genetically encoded fluorescent indicators enable optical measurement of neuromodulator dynamics, but available norepinephrine (NE) sensors draw from a limited subset of adrenergic receptor scaffolds. Here, we developed Alpha1DLight, an NE ...
A single-component optogenetic toolkit for reversible visualization and programmable control of microtubule dynamics [0.03%]
单组分光遗传学工具包揭示微管动力学的可逆视觉和程序控制
Tien-Hung Lan,Guolin Ma,Xiaoxuan Liu et al.
Tien-Hung Lan et al.
Microtubules form dynamic cytoskeletal scaffolds essential for intracellular transport, organelle positioning, and spatial organization of signaling. Their architecture and function are continuously remodeled through the concerted actions o...
SpaDiff denoises sequence-based spatial transcriptomics via diffusion process [0.03%]
基于序列的空间转录组学的去噪方法SpaDiff：通过扩散过程实现
Jiazhang Cai,Yongkai Chen,Luyang Fang et al.
Jiazhang Cai et al.
Spot-swapping in sequence-based spatial transcriptomics redistributes RNA molecules across neighboring spots, distorting spatial gene expression and biasing downstream analysis. We introduce SpaDiff, a mass-conserving denoising framework th...
Off-target effects of DREADD ligands revealed by an anesthesia emergence paradigm in mice [0.03%]
一种小鼠麻醉苏醒范式揭示了DREADD配体的脱靶效应
Miryam Moreno-Gomez,Guglielmo Foffani,Desire Humanes-Valera
Miryam Moreno-Gomez
Designer receptors exclusively activated by designer drugs (DREADDs) enable reversible control of specific neural circuits, but the pharmacological neutrality of their ligands is increasingly questioned. Here, we introduce an anesthesia eme...
Benchmarking dynamic analysis methods in diffusion-based single-molecule FRET [0.03%]
基于扩散的单分子FRET中的动态分析方法的基准测试
Joel A Crossley
Joel A Crossley
Single-molecule Förster resonance energy transfer (smFRET) provides nanometer-scale snapshots of biomolecular conformational dynamics, but extracting reliable dynamic information from diffusion-based measurements requires a careful choice ...
Kehan Chen,Ping Han,Zhe Lin et al.
Kehan Chen et al.
Tokenization is both a prerequisite and a central challenge for genomic language models, due to the inherent difficulty of delineating meaningful segments within continuous DNA sequences. We present GenART, a genomic language model framewor...
ABA-AA: A simple, reversible, and non-toxic anchor-away system for effective nuclear protein depletion [0.03%]
ABA-AA：一种简单、可逆且无毒的锚定撤离系统，用于有效耗尽核蛋白
Sofia Esteban-Serna,Tove Widén,Simon Seliner et al.
Sofia Esteban-Serna et al.
The anchor-away (AA) technique enables rapid depletion of nuclear proteins by tethering them to cytoplasmic anchors through rapamycin-induced heterodimerization. In Saccharomyces cerevisiae, this system is restricted to rapamycin-resistant ...
Mapping brain-wide monosynaptic inputs to single neurons with ROInet-seq [0.03%]
利用ROInet-seq技术绘制全脑单突触输入至单神经元的连接图
Zhige Lin,Osnat Ophir,Thorsten Trimbuch et al.
Zhige Lin et al.
Viral projection tracing strategies help build regional connectomes of mammalian brains. G-deleted rabies virus (ΔG-RV) establishes monosynaptic input connectivity but cannot distinguish networks at cell resolution. Here, we implement a ba...