Scalable nonparametric clustering with unified marker gene selection for single-cell RNA-seq data [0.03%]
具有统一标记基因选择的可扩展非参数聚类方法在单细胞RNA测序数据中的应用
Chibuikem Nwizu,Madeline Hughes,Michelle L Ramseier et al.
Chibuikem Nwizu et al.
Clustering is commonly used in single-cell RNA sequencing (scRNA-seq) to assess cellular heterogeneity, but standard methods often require user-specified heuristics and rely on post-selective differential expression analyses, which often le...
Ontology-aware DNA methylation classification with a curated atlas of human tissues and cell types [0.03%]
一种利用人类组织和细胞类型详图的基于本体的DNA甲基化分类方法
Mirae Kim,Ruth Dannenfelser,Yufei Cui et al.
Mirae Kim et al.
DNA methylation is a key regulatory mechanism reflecting both short- and long-term biological stimuli. While it has been widely used to study aging through disease-associated methylation shifts, its potential for revealing tissue-specific s...
A robust method for on-chip production and manipulation of lipid vesicles by inverted emulsion [0.03%]
一种制备和操控逆向乳液中脂质体的稳健方法研究
Naresh Yandrapalli,David T Gonzales,Weihua Leng et al.
Naresh Yandrapalli et al.
Lipid vesicles are important as minimal model systems for cellular compartmentalization. They drive major advances in deciphering biological mechanisms by molecular reconstitution; provide rational solutions for primitive compartmentalizati...
CBLN2 promoter enables genetic access to wide-field neurons of the tree shrew superior colliculus [0.03%]
CBLN2启动子可实现树鼩上丘广视野神经元的遗传获取
Arda Kipcak,Alev Erisir
Arda Kipcak
Wide-field (WF) neurons of the tectopulvinar pathway integrate retinal and cortical inputs via large dendritic arbors crucial for rapid visual motion detection. Previous studies identified potential marker genes for mouse WF neurons. Here, ...
Rapid expansion of primary human vocal fold epithelial cells via targeted pathway inhibition and anchorage-independent sphere culture [0.03%]
靶向途径抑制和锚独立球培养下人声带上皮细胞的快速扩增
Xudong Shi,Ryo Suzuki,Haiyan Lu et al.
Xudong Shi et al.
Vocal fold epithelial cells (VFEs) serve critical physiologic and immunologic functions at the boundary between the upper and lower airways but are difficult to maintain and expand in primary cultures. This technical challenge has impeded p...
Projection targeting with phototagging to study the structure and function of retinal ganglion cells [0.03%]
使用光标记的投影靶向技术研究视网膜节细胞的结构和功能
Martin O Bohlen,Andra M Rudzite,Tierney B Daw et al.
Martin O Bohlen et al.
Understanding the structure-function relationships across neurons is challenging, particularly when circuits are composed of dozens of distinct cell types. We refined an approach, called "projection targeting with phototagging", that allows...
Non-invasive ovulation tracking enables genetic engineering in wild rodents [0.03%]
无创性排卵监测使野生啮齿动物的基因组编辑成为可能
Joanna Buchthal,Emma J Chory,Zachary Hill et al.
Joanna Buchthal et al.
Many non-model rodent species are inaccessible to genetic engineering due to our limited understanding of their reproductive biology. Here, we present a low-cost, camera-based estrous-tracking technology that enables transgenesis in the whi...
Pietro Chiolerio,Beatrice Auletta,Camilla Pezzini et al.
Pietro Chiolerio et al.
Cancer cachexia, a devastating metabolic wasting syndrome affecting up to 80% of solid cancer patients, remains incurable despite advances in tumor biology understanding. This study introduces neuromuscular organoids (NMOs) derived from hum...
A 3D multi-compartment assembloid to study combined immune cell infiltration and cytotoxicity [0.03%]
一种用于研究联合免疫细胞浸润和细胞毒性的三维多隔室组装体模型
Eban A Hanna,Ashleigh J Crawford,Wenxuan Du et al.
Eban A Hanna et al.
Immune cell-mediated killing of cancer cells in a solid tumor is prefaced by a multi-step infiltration cascade of invasion, directed migration, and cytotoxic activities. Standard in vitro cytotoxicity assays measure immune cell killing in o...
Efficient global accuracy estimation for protein complex structural models using multi-view representation learning [0.03%]
基于多视角表示学习的蛋白质复合物结构模型全局精度高效估计方法
Dong Liu,Xuanfeng Zhao,Tianyou Zhang et al.
Dong Liu et al.
With the rapid advancement of protein structure prediction techniques and the explosive growth of predicted structural data, existing estimation of model accuracy (EMA) methods struggle to balance computational efficiency with estimation pe...