MalDeepSeq panel: A targeted ultra-deep sequencing approach to trace drug resistance markers in Plasmodium falciparum [0.03%]
针对恶性疟原虫抗药性标记物追踪的靶向超高深度测序方法(MalDeepSeq)面板
Yanka E A R Salazar,Antonio M Rezende,Maria C S B Puça et al.
Yanka E A R Salazar et al.
The emergence of drug-resistant Plasmodium falciparum highlights the need for tools to detect minor parasite subpopulations before resistant lineages expand. We developed and validated a targeted ultra-deep sequencing framework for the full...
Induced pluripotent stem cell-derived macrophages enable broad modeling of human inflammasome signaling [0.03%]
诱导多能干细胞源性巨噬细胞可广泛模拟人类炎性小体信号转导
Chloe M McKee,Melanie Cranston,Emma C McKay et al.
Chloe M McKee et al.
Macrophage models are a mainstay of inflammasome research; however, current in vitro models have significant limitations. Here, we generate induced pluripotent stem cell (iPSC)-derived macrophages (iMacs) to study inflammasome signaling and...
Rapid discovery of cell-surface glycosylation regulators using a lectin-based magnetic CRISPR screen [0.03%]
基于凝集素的磁性CRISPR筛选快速发现细胞表面糖基化调节因子
Jimmy Kim,Halen Kovacs,Simon Wisnovsky
Jimmy Kim
FACS-based CRISPR screening has emerged as a potent tool for dissecting the genetic networks that regulate cell-surface glycosylation. However, existing protocols can be tedious and are not compatible with many cell models. We developed a l...
A real-time FRET ubiquitin transfer assay for quantitative characterization of ternary complexes in targeted protein degradation [0.03%]
用于定量表征靶向蛋白降解三元复合物的实时FRET泛素转移测定法
Corey H Yu,Vi Dougherty,Dongwen Lv et al.
Corey H Yu et al.
Inhibiting or redirecting E3 ubiquitin ligase activity with drug-like small molecules is of growing therapeutic interest, yet broadly applicable and robust assays for quantifying E3-catalyzed ubiquitination remain limited. Here, we present ...
A high-throughput, end-to-end pipeline for extracellular miRNA biomarker discovery from human biofluids [0.03%]
一种高效的体外microRNA生物标志物发现的端到端流程：从人体生物流体分析入手
Abbas Hakim,Jennifer N Chousal,Srimeenakshi Srinivasan et al.
Abbas Hakim et al.
We developed a scalable pipeline for extracellular miRNA (ex-miRNA) profiling that integrates automated exRNA extraction, small RNA sequencing, and bioinformatic analysis including data processing and normalization. Automated extraction pro...
Neha Srinivas,Serap Erdogmus,Gurkan Mollaoglu
Neha Srinivas
Intratumoral heterogeneity (ITH) is the coexistence of diverse cancer cell states, genotypes, and microenvironmental niches within a single tumor and is a major driver of therapeutic resistance and disease progression. While the clinical im...
Vijayaraj Nagarajan,Reiko Horai,Guangpu Shi et al.
Vijayaraj Nagarajan et al.
IAN is an R package addressing the challenge of integrating, analyzing, and interpreting gene expression data using an artificial intelligence (AI) system. It leverages popular pathway (KEGG, WikiPathways, Reactome, and GO) and regulatory d...
Simple 3D-printed stirred bioreactor enhances retinal organoid production via improved oxygenation [0.03%]
一种简单的3D打印搅拌生物反应器通过改善供氧来增强视网膜器官生产
Kyle H Schwab,Philsang Hwang,Ki Yoon Nam et al.
Kyle H Schwab et al.
Static cultures, the standard for growing monolayer cells, were presumed suitable for and widely used during the initial adherent phase of retinal organoid (RO) differentiation. This assumption may be incorrect, as monolayer human pluripote...
Direct quantification of the metabolic heat output of individual Drosophila brains [0.03%]
单个果蝇大脑代谢热输出的直接量化
Kanishka Panda,Rohith Mittapally,Qianqian Chen et al.
Kanishka Panda et al.
Quantitative insights into brain metabolism are essential for advancing our understanding of the energy dynamics in the brain. Here, we present a nanowatt-resolution biocalorimeter capable of real-time metabolic heat output measurements of ...
Φ-Space ST: A platform-agnostic method to identify cell states in spatial transcriptomics studies [0.03%]
基于空间转录组学研究的细胞状态识别平台无关方法
Jiadong Mao,Jarny Choi,Kim-Anh Lê Cao
Jiadong Mao
We introduce Φ-Space ST, a platform-agnostic method to identify continuous cell states in spatial transcriptomics (ST) data using multiple scRNA-seq references. For ST with supercellular resolution, Φ-Space ST achieves interpretable cell-...