Mesenchymal stem cell-derived liver-on-a-chip reconstitutes human liver structure and function [0.03%]
由间充质干细胞衍生的肝脏芯片重构人类肝脏结构和功能
Anastasia Brooks,Haotian Yang,Deborah S Barkauskas et al.
Anastasia Brooks et al.
An in vitro model of human liver with functional integrity would advance liver research and enable personalized therapeutics. A microfluidic liver-on-a-chip can generate a functional hepatobiliary network using human liver cells and recapit...
Hiroaki Takesue,Satoshi Okada,Takashi Ito
Hiroaki Takesue
Gene amplification plays a critical role in evolution and disease and is widely utilized to overexpress valuable gene products in biotechnology. To broaden these applications, we previously developed break-induced replication (BIR)-mediated...
Volumetric denoising enables high-throughput volume electron microscopy and efficient downstream analysis [0.03%]
体素去噪可实现高通量体积电子显微镜和高效的后续分析
Bohao Chen,Fangfang Wang,Haoyu Wang et al.
Bohao Chen et al.
Volume electron microscopy (VEM) enables nanometer-resolution three-dimensional (3D) visualization of biological specimens via serial sectioning and imaging. Owing to limitations of downstream analysis, VEM datasets are often acquired at sl...
AAV-delivered TurboRFP enables streamlined tracing and analysis of corticospinal tract sprouting [0.03%]
AAV递送的TurboRFP使皮质脊髓束侧支形成的追踪和分析更简便流畅
Marc Hernaiz-Llorens,Camilo J Londoño,Ellen Wang et al.
Marc Hernaiz-Llorens et al.
Quantitative analysis of corticospinal tract (CST) sprouting after injury requires reliable labeling of long-range axons and fine collateral branches. Conventional biotinylated dextran amine (BDA) tracing has limited sensitivity and require...
Targeting the peripheral nervous system in adipose tissue with engineered AAV vectors [0.03%]
利用工程化AAV载体靶向脂肪组织的外周神经系统
Jake W Willows,Lindsey M Lazor,Gilian Gunsch et al.
Jake W Willows et al.
Adipose tissue innervation plays a critical role in regulating energy homeostasis and metabolic functions, yet targeted gene delivery to these peripheral nerves has remained challenging. We systematically evaluated both naturally occurring ...
Rapid and reversible regulation of cell cycle progression in budding yeast using optogenetics [0.03%]
利用光遗传学在 budding酵母菌中快速且可逆地调节细胞周期进程
Andriana Koutsoumpa,Catherine Loukogiannaki,Danny Incarnato et al.
Andriana Koutsoumpa et al.
The complexity of the eukaryotic cell cycle complicates experiment design and data interpretation, limiting our understanding of how cells coordinate cell cycle processes. Traditional perturbation methods, including knockouts, deletions, an...
ATP-Red enables single-cell bioenergetic phenotyping by flow cytometry [0.03%]
ATP-Red使能够通过流式细胞术进行单细胞生物能量表型分析
Jose Marin,Bharati Singh,Amanda Fuchs et al.
Jose Marin et al.
Most established bioenergetic assays lack single-cell resolution and may mask metabolic heterogeneity. The main flow-cytometry-based approach for bioenergetic analysis infers energetic state from protein synthesis, although translation can ...
A xeno-free human iPSC-derived prostate organoid platform for multilineage differentiation and genetic manipulation [0.03%]
一种无异种动物成分的人类多能干细胞来源的前列腺类器官体系及其分化和遗传操作功能
Neha Shaikh,Matthew Teasdale,Lewis J Walker et al.
Neha Shaikh et al.
Current prostate organoid models rely on tissue-derived material or animal components and lack epithelial and stromal complexity. We defined a xeno-free system to generate human prostate organoids from induced pluripotent stem cells with co...
Trajectory-informed gene feature selection in single-cell analysis with SEEK-VFI [0.03%]
基于SEEK-VFI的单细胞数据分析中具有运动轨迹信息的基因特征选择方法研究
Rebecca Danning,Zheng Tracy Ke,Xihong Lin et al.
Rebecca Danning et al.
The prioritization of highly variable genes is an important step in single-cell trajectory inference. However, when variability arises from a continuous latent cell development trajectory, standard methods may fail to differentiate trajecto...
Chenxiang Li,Wenjing Gao,Qiqiao Zhang et al.
Chenxiang Li et al.
Influenza-like illness (ILI) remains a persistent global health challenge, necessitating accurate forecasting tools for timely public health response. This study systematically benchmarks fine-tuned large language models (LLMs), e.g., Llama...