HyPIC-3D enables characterization of migratory cancer cell subpopulations in 3D hypoxic microenvironments [0.03%]
HyPIC-3D可在三维缺氧微环境中表征迁移性癌细胞亚群
Luana Schito,Sergio Rey-Keim
Luana Schito
Malignant tumors harbor cancer cell subpopulations with heterogeneous migratory behaviors. Identifying the molecular programs specifying whether these subpopulations migrate or remain stationary is essential for preventing metastasis and re...
In vivo visualization of bioactive iron and oxygen using LiON, the labile iron and oxygen notifier [0.03%]
用于活体可视化生物活性铁和氧的LiON（游离铁和氧示踪剂）标记体系
Ayato Maeda,Akihiro Nita,Shoko Sashiyama et al.
Ayato Maeda et al.
Iron and oxygen are essential for numerous biological processes, yet monitoring their bioactive intracellular dynamics in vivo remains challenging due to the limitations of current detection methods. Here, we introduce the labile iron and o...
A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study [0.03%]
基于多重PCR的测序方法用于血浆样本诊断和区分泡型和囊型包虫病的概念验证研究
Yanping Zhao,Yeqin Wang,Ming Zhi et al.
Yanping Zhao et al.
Echinococcosis poses a significant public health threat, and its diagnosis relies on imaging. Non-invasive plasma detection of Echinococcus cell-free DNA (cfDNA) offers a promising diagnostic approach. We developed a low-cost multiplex PCR ...
Human cerebral organoids with microglia and vasculature model glioma stem cell interactions and radiotherapy response [0.03%]
含小胶质细胞和血管的脑类器官模拟胶质瘤干细胞相互作用及放射治疗反应
Jérémy Raguin,Noa Legrand,Thierry Kortulewski et al.
Jérémy Raguin et al.
Most human brain organoid models derived from induced pluripotent stem cells (iPSCs) lack vascular and/or immune components, despite their critical roles in maintaining brain homeostasis and contributing to pathophysiological processes. We ...
Resilience of recombinant antibiotic resistance gene-containing plasmids against common cell culture disposal methods [0.03%]
耐药基因重组质粒在细胞培养废弃处理过程中的稳定性研究
Austin Gluth,Christian Zmasek,Stephen Chiu et al.
Austin Gluth et al.
Antibiotics have saved an untold number of people and animals since penicillin's miraculous discovery in 1928. In the following half-century, progressive discoveries involving antibiotic resistance genes (ARGs), the microorganisms responsib...
High-precision stress prediction using deep energy network enhanced by stress equilibrium with Delaunay integration on refined grid [0.03%]
基于精细网格上Delaunay积分的应力平衡增强型深度能量网络高精度应力预测方法
Yongqiang Ye,Dongming An,Hui Li et al.
Yongqiang Ye et al.
We present the deep energy method enhanced by stress equilibrium (DEM-SE), a physics-informed neural network (PINN) architecture for high-precision prediction of complex stress fields in elastic plates. The method calculates total potential...
DRIFT-EM enables direct wafer retrieval of ultrathin serial sections for large-volume electron microscopy [0.03%]
采用大视场电子显微镜直接回收超薄连续切片的漂移方法
Nelson Medina,Joseph V Gogola,Kevin Boergens et al.
Nelson Medina et al.
Large-volume serial electron microscopy (vEM) has transformed neuroscience by enabling reconstructions of neural circuits at synaptic resolution. Approaches to collecting libraries of ultrathin sections required for producing vEM datasets h...
Noninvasive focal gene transfer of chemogenetic proteins in the primate brain [0.03%]
灵长类动物大脑中化学遗传蛋白聚焦非病毒基因转移
Michael R Corigliano,T Vincenza Parks,Simeon S Guretse et al.
Michael R Corigliano et al.
We leverage our recent development of transcranial focused ultrasound (tFUS) for noninvasive, focal delivery of adeno-associated viruses (AAVs)-encoding excitatory designer receptors exclusively activated by designer drugs (DREADDs)-to marm...
Inferring cell-specific gene regulatory networks based on causal graph embedding [0.03%]
基于因果图嵌入的细胞特异性基因调控网络推理方法研究
Guojie Li,Rui Qiao,Yunnuo Xu et al.
Guojie Li et al.
Gene regulatory networks (GRNs) at single-cell resolution provide a fundamental framework for understanding cellular functions and regulatory mechanisms. However, existing methods often focus on regulatory relationships among genes while ov...
Integrated multi-dimensional modeling of non-model bacteria identifies engineering targets for acarbose biosynthesis optimization [0.03%]
整合多维度建模非模式细菌发现 engineered 靶点以优化壳聚糖生物合成
Feifei Cai,Shijie Zhang,Yang Dai et al.
Feifei Cai et al.
Metabolic engineering for high-value compounds such as acarbose, a diabetes drug, requires systematic understanding of metabolic regulation. Here, we applied a multi-dimensional systems biology approach in Actinoplanes sp. SE50/110, a non-m...