Protocol for biomass and carbon storage estimation in tropical rainforests via remote sensing imagery [0.03%]
基于遥感影像的热带雨林生物量和碳储量估算技术规范
Dung Trung Ngo,Hoi Dang Nguyen,Hieu Huu Viet Nguyen et al.
Dung Trung Ngo et al.
Here, we present a protocol for estimating biomass and carbon sequestration in tropical rainforests. We describe steps for combining remotely sensed data from Sentinel-2 imagery with data collected from standard field surveys. This protocol...
Protocol for differentiation of vascular smooth muscle cells from human iPSCs and their application in CRISPRa-mediated gene regulation [0.03%]
人多能干细胞向血管平滑肌细胞定向分化及CRISPRa调控基因表达的 protocol
Laura Priesmeier,Malte Tiburcy,Laura Cecilia Zelarayán
Laura Priesmeier
Directed differentiation of human induced pluripotent stem cells (hiPSCs) holds major promise for the development of disease models, drug screening platforms, and regenerative medicine. Here, we provide a step-by-step highly reproducible pr...
Protocol to study the role of endogenously produced itaconate using CRISPR-Cas9 technology in THP-1 cells [0.03%]
基于CRISPR-Cas9技术研究内源琥珀酸产生对THP-1细胞的作用机制
Luke A Bourner,Katie A Acken,Haiyan Long et al.
Luke A Bourner et al.
Itaconate mimetics inadequately represent endogenous itaconate, a negative regulator of innate immune-driven pro-inflammatory cytokines. We present a CRISPR-Cas9 protocol to delete a 4-nucleotide region in the immunoresponsive gene 1 (IRG1)...
Protocol to investigate human mitochondrial transcription initiation by integrating biochemical and cryo-EM approaches [0.03%]
结合生化和冷冻电子显微镜方法研究人类线粒体转录起始的协议
Yogeeshwar Ajjugal,Quinten Goovaerts,Jiayu Shen et al.
Yogeeshwar Ajjugal et al.
Here, we present a protocol to investigate the mechanism and regulation of human mitochondrial transcription initiation by integrating biochemical and cryoelectron microscopy (cryo-EM) approaches. We describe the steps for expressing and pu...
Protocol to express anti-viral nanobodies and antigenic proteins anchored on the bacterial cell surface through the engineering of probiotic Escherichia coli Nissle 1917 [0.03%]
工程益生菌大肠杆菌Nissle 1917在细菌细胞表面表达抗病毒纳米抗体和抗原蛋白的协议
Nitin S Kamble,Abram Canowitz,Nathan Muck et al.
Nitin S Kamble et al.
Here, we present a protocol to express anti-viral nanobodies or antigenic proteins anchored on the bacterial cell surface through the engineering of probiotic E. coli Nissle 1917 (EcN). We describe steps for bacterial transformation, protei...
Protocol for necropsy and tissue processing of the common marmoset, Callithrix jacchus, for histopathological analysis [0.03%]
用于食蟹猴尸体剖检和组织处理的协议以进行组织病理学分析
Na-Young Lee,Na Yun Lee,Jina Kwak et al.
Na-Young Lee et al.
The common marmoset (Callithrix jacchus) is an important nonhuman primate model in biomedical research, but the lack of a standardized necropsy protocol limits reproducibility and data quality. Here, we present a protocol for necropsy and t...
Protocol for conditional gene expression in Magnaporthe oryzae via fungal nitrate reductase promoter replacement [0.03%]
通过真菌硝酸还原酶启动子替换在稻瘟茵中进行条件基因表达的协议
Mostafa Rahnama,Justin King
Mostafa Rahnama
Functional genomic studies rely on controlled gene expression, which is challenging for essential genes or those active only under specific conditions. We provide a protocol for generating Magnaporthe oryzae (synonym of Pyricularia oryzae) ...
Protocol for identifying the BZR1 interactome under starvation in Arabidopsis by metabolic stable isotope labeling quantitative IP-MS [0.03%]
基于代谢稳定同位素标记的定量免疫沉淀质谱技术研究拟南芥胁迫条件下BRZ1互作蛋白组学的方案
Zhenzhen Zhang,Zhi-Yong Wang
Zhenzhen Zhang
Identification of interacting proteins, particularly those mediating protein degradation, remains technically challenging. The transcription factor BZR1 in Arabidopsis is activated by brassinosteroid (BR) signaling to promote plant growth a...
Protocol for minimizing off-target neuronal activation during optical stimulation in vivo [0.03%]
体内光学刺激中最小化脱靶神经元激活的协议
Simon Weiler,Mateo Vélez-Fort,Luke O&#x;Hara et al.
Simon Weiler et al.
In vivo optogenetic stimulation is a powerful approach for dissecting neuronal circuit function, yet it can inadvertently lead to activation of endogenous retinal opsins, producing off-target responses that propagate to the cortex and there...
Protocol to study adult neurogenesis in fresh-frozen human hippocampal tissue using an immunofluorescence quantitative approach [0.03%]
基于免疫荧光定量技术的新鲜冷冻人脑海马组织神经发生研究方法学探索
Marta Gallardo-Caballero,Berenice Márquez-Valadez,María Llorens-Martín
Marta Gallardo-Caballero
Adult hippocampal neurogenesis (AHN) results in the generation of new neurons throughout adulthood, thereby contributing to key hippocampal functions such as learning and mood regulation. Here, we present a protocol for the immunofluorescen...