Protocol to study the role of endogenously produced itaconate using CRISPR-Cas9 technology in THP-1 cells
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Itaconate mimetics inadequately represent endogenous itaconate, a negative regulator of innate immune-driven pro-inflammatory cytokines. We present a CRISPR-Cas9 protocol to delete a 4-nucleotide region in the immunoresponsive gene 1 (IRG1), ablating ACOD1 (th... ...
