Protocol to investigate fibroblastic reticular cell-immune cell interaction in human lung cancer [0.03%]
人肺癌中成纤维网状细胞-免疫细胞相互作用的检测方法
Chrysa Papadopoulou,Hung-Wei Cheng,Almut Lütge et al.
Chrysa Papadopoulou et al.
Fibroblastic reticular cells (FRCs) play critical roles in the generation of anti-tumor immune responses. Here, we present a protocol for characterizing FRCs in human lung cancer. This step-by-step protocol involves enrichment of rare FRCs ...
Protocol for the differentiation of induced pluripotent stem cells into retinal pigment epithelial cells [0.03%]
诱导多能干细胞分化成为视网膜色素上皮细胞的规范流程
Mark Zorin,Rike Ewerling-Hähnel,Kerstin Nagel-Wolfrum
Mark Zorin
Induced pluripotent stem cells (iPSCs) are emerging as a valuable system for modeling tissues and organs. Here, we describe a highly scalable protocol for the differentiation of iPSCs into retinal pigment epithelium (RPE), including a new s...
Protocol for endolysosomal proteomics in goat atrial tissue using a refined density-gradient approach [0.03%]
山羊心房组织内溶酶体蛋白组学的协议：采用改进的密度梯度法
Thamali Ayagama,Georgina Berridge,Roman Fischer et al.
Thamali Ayagama et al.
Here, we present a protocol to analyze endolysosomal (EL) proteins in goat atrial fibrillation (AF) tissue using a modified density-gradient, proteomic-based fractionation. This method separates organelles from tissue lysate, isolating sarc...
Huan Zhao,Xinyue Wang,Liangsheng Wang
Huan Zhao
Chloroplasts are fundamental plant organelles in green plants and algae. As an endosymbiosis-originated organelle, most chloroplast proteins are encoded by nuclear genes, translated in the cytosol, and imported into the chloroplast. Here, w...
Protocol for preparing natural peptidoglycan nanoparticles and purifying plant-derived antigens [0.03%]
制备天然肽聚糖纳米颗粒和提纯植物抗原的方案
Hui-Xin Meng,Shi-Yu Ren,Xue-Jiao Xu et al.
Hui-Xin Meng et al.
Purifying plant-derived proteins is challenging due to host contaminants and high downstream costs. Here, we present a protocol for the preparation of natural peptidoglycan nanoparticles (NPNs) from lactic acid bacteria (LAB) and their use ...
Protocol for structural analysis of amyloid fibrils using hydrogen-deuterium exchange mass spectrometry [0.03%]
氢氘交换质谱鉴定淀粉样纤维结构的实验方案
Qian Meng,Yuliang Han,Hu Zhou et al.
Qian Meng et al.
Amyloid fibrils are common in many diseases. Here, we present a protocol that uses α-synuclein fibrils, a Parkinson's disease hallmark, to demonstrate hydrogen-deuterium exchange (HDX) mass spectrometry (HDX-MS) for amyloid protein structu...
Protocol for single-molecule analysis of synaptic protein complex-mediated vesicle recruitment [0.03%]
单分子分析突触蛋白质复合物介导的囊泡募集方案
Akshay Kapadia,Anne-Sophie Hafner
Akshay Kapadia
Single-molecule pull-down (SIM-Pull) combined with total internal reflection fluorescence (TIRF) microscopy enables direct visualization of proteins and multi-protein complexes. Here, we present an extended SIM-Pull protocol for analyzing p...
Protocol for obtaining polyhydroxyalkanoates from microbial cultures: Production, quantification, and analytical assays [0.03%]
通过微生物培养获取聚羟基烷酸酯的方案：生产、定量和分析测定
Maria-Tsampika Manoli,Natalia Hernández-Herreros,Virginia Rivero-Buceta et al.
Maria-Tsampika Manoli et al.
Polyhydroxyalkanoates (PHAs), a family of natural bacterial biodegradable biopolymers, constitute a promising alternative to conventional plastics. Here, we present a protocol to monitor bacterial growth and biopolymer accumulation in shake...
Protocol for generation of endothelial cells from human-derived iPSCs and integration into mouse brain explants [0.03%]
人源性iPSCs分化内皮细胞及整合到小鼠脑片的实验方案
Iza Erzar,Nora Noll,Roberta Lugano et al.
Iza Erzar et al.
The rapid and reliable generation of endothelial cells from induced pluripotent stem cells (iPSCs) is a keystone for biomedical research and regenerative medicine. Here, we present a protocol for human iPSC genetic engineering, ETS variant ...
Protocol for wide-field brainstem imaging largely preserving the overlying cerebellum [0.03%]
一种广泛的大脑干成像方案，最大程度地保存其上方的小脑组织
Azumi Hatakeyama,Chihiro Ito,Daisuke Yamada et al.
Azumi Hatakeyama et al.
The nucleus tractus solitarii (NTS) is a brainstem structure that receives information from various internal organs, relaying it to brain networks that regulate emotions and metabolism. Here, we describe a step-by-step experimental protocol...