Protocol for the quantitative imaging and segmentation of spatial patterns in Hibiscus trionum petals [0.03%]
Hibiscus trionum花瓣空间图案定量成像和分割的协议
Lucie Riglet,Edwige Moyroud
Lucie Riglet
Most developmental processes underpinning the diversity of floral forms remain unexplored due to limitations in current imaging approaches. Here, we present a protocol to spatially analyze tissue-wide cell growth dynamics through confocal i...
Protocol for identifying properties of ERBB receptor antagonists using the barcoded ERBBprofiler assay [0.03%]
用于识别ERBB受体拮抗剂特性的条形码ERBB检测方法协议
Lukša Popović,Moritz J Rossner,Michael C Wehr
Lukša Popović
Published Erratum
STAR protocols. 2026 Feb 3;7(1):104372. DOI:10.1016/j.xpro.2026.104372 2026
A high-parameter spectral cytometry protocol for immune subset and functional characterization of cryopreserved human blood with 49 biomarkers [0.03%]
一种高参数谱域细胞计数术：使用49种生物标志物对冷冻的人类血液的免疫亚型和功能进行表征的方法
Austin P Wright,Marissa Lindaman,Elaine Huang et al.
Austin P Wright et al.
Comprehensive immune profiling of human blood is a critical approach for evaluating immune responses following therapeutic intervention in clinical trials. Here, we present a protocol to assess leukocyte lineages, activation status, and sel...
Protocol for tailored in vitro neuronal networks on high-density microelectrode arrays with polydimethylsiloxane microstructures [0.03%]
基于聚二甲基硅氧烷微结构的高密度微电极阵列上构建定制型体外神经网络的方案
Anna-Christina Haeb,Hideaki Yamamoto,Paul Roach et al.
Anna-Christina Haeb et al.
Complementary metal-oxide-semiconductor (CMOS)-based high-density microelectrode arrays (HD-MEAs) enable neuronal recordings with high spatiotemporal resolution. However, integrating polydimethylsiloxane (PDMS) microstructures onto HD-MEA s...
Protocol for intraventricular injection of retinoids and hypothalamic application of viral vectors in the rat and mouse brain [0.03%]
小鼠和大鼠脑室视黄醇注射及下丘脑病毒载体应用的方案
Peter I Imoesi,Cristian M Olarte-Sánchez,Lora Heisler et al.
Peter I Imoesi et al.
The brain, specifically the hypothalamus, is a key regulator of the homeostatic balance of numerous biomolecules in the body via its control of the neuroendocrine system. Here, we present a protocol to study body vitamin A balance by inject...
Protocol for spatial quantitative analysis of cell division events across the epidermis of developing Hibiscus trionum petals [0.03%]
扶桑三色牡丹花瓣表皮细胞分裂事件的空间定量分析协议
Lucie Riglet,Katharina Schiessl,Edwige Moyroud
Lucie Riglet
Characterizing cell proliferation across developing tissue provides critical insights into how spatially regulated growth shapes mature organs. Here, we present a protocol for high-resolution mapping of cell division events across the epide...
Protocol for the simultaneous detection of nuclear long non-coding RNAs and proteins in human iPSC-derived cardiomyocytes [0.03%]
人诱导多能干细胞源性心肌细胞中长链非编码RNA和蛋白的联合检测方法
Giulia Buonaiuto,Tiziana Santini,Monica Ballarino
Giulia Buonaiuto
Nuclear-enriched long non-coding RNAs (lncRNAs) can regulate gene expression by interacting with specific RNA-binding proteins (RBPs). Here, we present a protocol to directly image these lncRNAs at the single-molecule level together with th...
Protocol to study mRNA levels during endoreplication in Arabidopsis thaliana through nuclei extraction and a sorting strategy [0.03%]
通过核提取和分选策略研究拟南芥在内多倍体过程中的mRNA水平的协议
Karin Rothkegel,Alex Cabrera Núñez,Rodrigo A Gutiérrez
Karin Rothkegel
In plants, differentiation and expansion of some cell types is accompanied by exit from cell division and onset of endoreplication. Here, we describe a protocol to study changes in mRNA levels during endoreplication in Arabidopsis thaliana ...
Protocol for the generation and xenotransplantation of human induced pluripotent stem cell-derived neural progenitor cells into the mouse forebrain [0.03%]
人诱导多能干细胞来源神经前体细胞的生成及其异种移植到小鼠大脑皮质的方法学论文
Julie J McInvale,Chloe E Ayers,Hemanta Sarmah et al.
Julie J McInvale et al.
Here, we present a protocol for the small-molecule-driven derivation of dopaminergic and GABAergic neurons from neural progenitor cells (NPCs) differentiated from human induced pluripotent stem cells and for stereotactic xenotransplantation...
Protocol for the isolation and immunoprecipitation of cell surface proteins from Drosophila melanogaster pupae [0.03%]
果蝇包壳细胞表面蛋白质的分离和免疫沉淀协议
Anne Bormann,Marina Bigl,Nicole Scholz
Anne Bormann
Transmembrane proteins are notoriously difficult to isolate from tissue samples due to their hydrophobic nature and often low abundance. Here, we describe a protocol for the isolation, enrichment, and detection of the high-molecular-weight ...