Protocol for isotype-resolved 3D imaging and quantification of bone marrow plasma cells in murine femurs [0.03%]
小鼠股骨骨髓浆细胞的同种型分辨三维成像和量化方案
Abdouramane Camara,Baizhi Chen,An Qi Xu et al.
Abdouramane Camara et al.
Bone marrow plasma cells maintain durable antibody responses, but their tissue localization and morphology are difficult to quantify in situ. Here, we present a protocol for isotype-resolved 3D imaging and quantification of bone marrow plas...
Click-code-seq protocol for genome-wide single-nucleotide-resolution mapping of major types of DNA modifications [0.03%]
单核苷酸分辨率全基因组DNA修饰图谱的Click-Code-Sequencing技术
Lucie Lefevre,Emilie A Aghajani,Navnit K Singh et al.
Lucie Lefevre et al.
DNA modifications are central to carcinogenesis, chemotherapy drug action, neurodegeneration, and aging. Here, we present a protocol for click-code-seq, a next-generation sequencing technique for genome-wide single-nucleotide-resolution map...
Protocol for proteomic profiling of hypusinated protein candidates using a clickable spermidine probe [0.03%]
使用可点击鸟氨酸类似物探针进行蛋白质组范围内泛素化蛋白质的富集和鉴定实验方案
Tian Zhang,Jianlong Li,Xinbo Hu et al.
Tian Zhang et al.
Hypusination is a unique posttranslational modification in which deoxyhypusine synthase (DHPS) transfers an aminobutyl moiety from spermidine to specific lysine residues, followed by deoxyhypusine hydroxylase (DOHH)-mediated hydroxylation. ...
Protocol for surgical stress-promoted colorectal cancer liver metastasis in mice [0.03%]
小鼠结直肠癌肝转移的手术应激促发标准方案
Jian Han,Peter Tsung,Natalie Blatz et al.
Jian Han et al.
Although hepatectomy can be therapeutic, ischemia-reperfusion (I/R) caused by temporary interruption of blood flow creates an inflammatory microenvironment that facilitates tumor-cell seeding and postoperative metastasis. Here, we present a...
Protocol for untargeted LC-MS/MS metabolomics annotation and differential abundance analysis using the SIRIUS Python client [0.03%]
基于SIRIUS python客户端的非靶向液相色谱质谱代谢组学鉴定及差异分析协议
Jonas A Emmert,Sebastian Böcker,Markus Fleischauer
Jonas A Emmert
We present a protocol for programmatic untargeted LC-MS/MS metabolomics using PySirius, the Python client for SIRIUS. We describe steps for importing data with LC-MS feature alignment, and annotating features with molecular formulas, struct...
Protocol for live-imaging of blood flow and vascular permeability in zebrafish larvae [0.03%]
关于活体观察斑马鱼幼鱼血液流动和血管通透性的方案
Preethi Rajamannar,Gil Levkowitz
Preethi Rajamannar
Cerebral vascular dynamics are critical to maintain brain homeostasis, and the fenestrated capillaries of the hypophysis (also known as the pituitary) exhibit unique structural and functional features that support brain-to-periphery neuroen...
Elena S Philonenko,Baoyun Zhang,Eugene Albert et al.
Elena S Philonenko et al.
Human pluripotent stem cells (hPSCs) provide a powerful platform for generating T cell progenitors and investigating hematopoietic stem cell (HSC)-independent T cell development. Here, we present a protocol for the directed differentiation ...
Protocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slices [0.03%]
用于急性脑切片中单个突触多巴胺释放动力学的荧光假神经递质活体成像协议
Patrick Cottilli,Eugene V Mosharov,Michael J Devine et al.
Patrick Cottilli et al.
Striatal dopaminergic (DA) axons have many en passant boutons with synaptic vesicles, but only a fraction display exocytosis. Here, we present a protocol for live imaging of murine DA axons using fluorescent false neurotransmitter 200 (FFN2...
Protocol for identification of intronic lariat RNAs by RNA deep sequencing in Arabidopsis thaliana [0.03%]
拟南芥中通过RNA深度测序鉴定内含子环状RNA的协议
Haoran Ge,Xiaotuo Zhang,Qi Tang et al.
Haoran Ge et al.
Lariat RNAs formed from excised introns during RNA splicing typically undergo DBR1-mediated debranching and degradation; however, many lariat RNAs accumulate stably in various organisms. Here, we present a protocol for constructing RNA sequ...
Protocol for tumor detection in mice using β-glucuronidase targeted breath analysis [0.03%]
基于β-葡萄糖醛酸酶的呼吸分析小鼠肿瘤检测技术标准
Guochao Wu,Tianran Si,Han Jin
Guochao Wu
Early tumor detection is critical for improving cure rates. Here, we present a protocol for tumor detection in mice using a targeted breath-analysis strategy in which phenyl-β-D-glucuronide specifically targets β-glucuronidase, which is h...