Protocol for preparation of a synaptosome-enriched fraction to profile presynaptic RNA content in adult Drosophila brains [0.03%]
Lauren Blot,Agnès Loubat,Marine Dehecq et al.
Lauren Blot et al.
Neuronal synapses contain rich repertoires of RNAs that can be used for onsite translation and modulation of their proteomes. Here, we present a protocol to recover a synaptosome-enriched fraction adapted to presynaptic RNA profiling, start...
Protocol to quantify cell-surface vimentin on senescent human chondrocytes and other cell types via a cell-based ELISA [0.03%]
Jana Riegger,Hartmut Geiger
Jana Riegger
Surface markers for the identification and potential targeting of senescent cells are limited and typically assessed via flow cytometry. Here, we present a protocol to quantify cell-surface vimentin (CSV) as a membrane-associated marker of ...
Protocol for quantifying silent ribosome induction in yeast and mammalian cells using polysome profiling [0.03%]
Sayanur Rahaman,Samuel Mondal,Nicole Schiffelholz et al.
Sayanur Rahaman et al.
Translationally silent ribosomes lack bound mRNA and are difficult to quantify. Here, we present a protocol to measure silent ribosome induction under diverse conditions in yeast and mammalian cells. We describe steps to isolate polysome-pr...
Protocol to study regeneration in human pancreatic slices using viral tracing, extended culture, dissociation, and functional Ca2+ influx techniques [0.03%]
Silvia Álvarez-Cubela,Isabella Altilio,Dagmar Klein et al.
Silvia Álvarez-Cubela et al.
Human pancreatic slices provide an organotypic platform that preserves native cell interactions for functional studies under near-physiological conditions. We present a protocol to regenerating human pancreatic slices, including lentiviral ...
Protocol for streamlining genotyping of germline-transmissible mutants from genome editing by using a parallel qPCR-based index and R analysis [0.03%]
Emeric M Louis,Liezhen Fu,Nga Luu et al.
Emeric M Louis et al.
Targeted genome editing using CRISPR-Cas, ZFNs, or TALENs enables precise gene function studies but often produces point mutations or insertions or deletions (indels) that are difficult to detect by conventional PCR. We developed a parallel...
Protocol to generate an allo-transplantable tumor in mouse models using the YUMM1.7 cell line [0.03%]
Ahmed Rokan,Somaya NoorSaeed,Ariberto Fassati
Ahmed Rokan
Here, we present a protocol to generate an allo-transplantable tumor in mouse models using the YUMM1.7 cell line. We describe steps for mouse breeding using syngeneic C57BL/6 and allogeneic BALB/c mice, injecting the YUMM1.7 melanoma cell l...
Ilaria Chiaradia,Jerome Boulanger,Sofie Blomberg Elmkvist et al.
Ilaria Chiaradia et al.
Neural organoids can exhibit variability in both tissue shape and tissue identity. Here, we present a pipeline for rapid, protocol-agnostic quality control screening of brain organoids based on their overall gross morphology. We describe a ...
Protocol for generation of brain metastasis mouse model using stereotactic intracranial tumor-derived cells and modified intracarotid injection [0.03%]
Thi-Anh-Thuy Tran,Young-Hee Kim,Kyung-Hwa Lee et al.
Thi-Anh-Thuy Tran et al.
Here, we present a protocol for establishing a reproducible mouse model of brain metastasis. We describe the steps for preparing and injecting tumor cells into the internal carotid artery to achieve direct cerebral circulation delivery. We ...
Protocol for detecting in vitro riboswitch conformational switching using a fluorescence anisotropy single-stranded RNA-targeting approach [0.03%]
Amanda Clairoux,Maira Rivera,Maureen McKeague et al.
Amanda Clairoux et al.
We present a high-sensitivity fluorescence anisotropy-based protocol (fluorescence anisotropy single-stranded targeting [FASST]) to monitor in vitro ligand-induced conformational switching of riboswitches. We first describe the sequence des...
Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy [0.03%]
Anna Jaeschke,Matt S Hepburn,Alireza Mowla et al.
Anna Jaeschke et al.
Here, we present a protocol combining quantitative micro-elastography (QME) with transmitted light (TL) and immunofluorescence (IF) microscopy to study ovarian elasticity and composition in situ. We describe steps for excising murine ovarie...