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Protocol for fluorescent false neurotransmitter live imaging of dopamine release dynamics from individual synapses in acute brain slices

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Striatal dopaminergic (DA) axons have many en passant boutons with synaptic vesicles, but only a fraction display exocytosis. Here, we present a protocol for live imaging of murine DA axons using fluorescent false neurotransmitter 200 (FFN200), a vesicular mon... ...