Dmitrii Y Travin,Konstantin Severinov,Svetlana Dubiley
Dmitrii Y Travin
For most antimicrobial compounds with intracellular targets, getting inside the cell is the major obstacle limiting their activity. To pass this barrier some antibiotics mimic the compounds of specific interest for the microbe (siderophores...
Weijun Gui,Gregory A Davidson,Zhihao Zhuang
Weijun Gui
Ubiquitination is an important protein post-translational modification regulating many cellular processes in eukaryotes. Ubiquitination is catalyzed by a three-enzyme cascade resulting in the conjugation of the C-terminal carboxylate of ubi...
Design and Synthesis of Metabolic Chemical Reporters for the Visualization and Identification of Glycoproteins [0.03%]
用于糖蛋白的可视化与鉴别的代谢型化学报告器的设计和合成
Nichole J Pedowitz,Matthew R Pratt
Nichole J Pedowitz
Glycosylation events play an invaluable role in regulating cellular processes including enzymatic activity, immune recognition, protein stability, and cell-cell interactions. However, researchers have yet to realize the full range of glycan...
A two-trick pony: lysosomal protease cathepsin B possesses surprising ligase activity [0.03%]
一只耍俩把戏的 pony：溶酶体蛋白酶 cathepsin B 具有令人惊讶的连接酶活性
Tyler R Lambeth,Zhefu Dai,Yong Zhang et al.
Tyler R Lambeth et al.
Cathepsin B is an important protease within the lysosome, where it helps recycle proteins to maintain proteostasis. It is also known to degrade proteins elsewhere but has no other known functionality. However, by carefully monitoring peptid...
Corina Mathew,R Gregor Weiß,Christoph Giese et al.
Corina Mathew et al.
A hallmark of N-linked glycosylation in the secretory compartments of eukaryotic cells is the sequential remodeling of an initially uniform oligosaccharide to a site-specific, heterogeneous ensemble of glycostructures on mature proteins. To...
Bioorthogonal protein labelling enables the study of antigen processing of citrullinated and carbamylated auto-antigens [0.03%]
生物正交蛋白质标记使瓜氨酸和氨基甲酰化自身抗原的抗原处理的研究成为可能
Tyrza van Leeuwen,Can Araman,Linda Pieper Pournara et al.
Tyrza van Leeuwen et al.
Proteolysis is fundamental to many biological processes. In the immune system, it underpins the activation of the adaptive immune response: degradation of antigenic material into short peptides and presentation thereof on major histocompati...
An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells [0.03%]
用于活细胞中酚硫酸转移酶SULT1A1检测的荧光传感器
Regina A Baglia,Kira R Mills,Koushambi Mitra et al.
Regina A Baglia et al.
Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here...
Proteolysis targeting chimeras (PROTACs) come of age: entering the third decade of targeted protein degradation [0.03%]
蛋白降解靶向嵌合体(PROTAC)技术进入发展新阶段：靶向蛋白降解技术迎来第三个十年
Michael J Bond,Craig M Crews
Michael J Bond
With the discovery of PROteolysis TArgeting Chimeras (PROTACs) twenty years ago, targeted protein degradation (TPD) has changed the landscape of drug development. PROTACs have evolved from cell-impermeable peptide-small molecule chimeras to...
Antibody recruiting molecules (ARMs): synthetic immunotherapeutics to fight cancer [0.03%]
抗体募集分子（ARM）：抗癌的合成免疫治疗药物
Silvia Achilli,Nathalie Berthet,Olivier Renaudet
Silvia Achilli
Antibody-recruiting molecules (ARMs) are one of the most promising tools to redirect the immune response towards cancer cells. In this review, we aim to highlight the recent advances in the field. We will illustrate the advantages of differ...
Thibault J Harmand,Ashraful Islam,Novalia Pishesha et al.
Thibault J Harmand et al.
In vivo imaging has become in recent years an incredible tool to study biological events and has found critical applications in diagnostic medicine. Although a lot of efforts and applications have been achieved using monoclonal antibodies, ...