Illuminating spatial dynamics of glutamine metabolism with a sensitive genetically encoded biosensor [0.03%]
一种灵敏的谷氨酰胺代谢遗传编码生物传感器及其在揭示空间动态中的应用
Jack M Scully,Michael J Sun,Siddharth Das et al.
Jack M Scully et al.
Glutamine is the most abundant amino acid in serum, used as a key nutrient by cells for protein synthesis, energy production, carbon and nitrogen metabolism, and cellular redox balance. The use of glutamine in the cell is highly compartment...
Discovery of low molecular weight protein-binding fragments from bead-displayed libraries: application to monomeric proteins [0.03%]
用于单体蛋白的珠展示库低分子量结合片段的发现及应用
Joel Tong,Isuru M Jayalath,James V Parsons et al.
Joel Tong et al.
Fragment-based drug discovery (FBDD) is a powerful workflow for the development of drug candidates and probe molecules that begins with the discovery of one or more low affinity, low molecular weight ligands for a protein of interest (POI)....
Amrita Date,Archie Wall,Hannah Kiely-Collins et al.
Amrita Date et al.
The dysregulation of protein-protein interactions (PPIs) in disease states is well established, yet they are challenging to target, owing to the large surface area and featureless nature of protein binding interfaces. For targeting helix-me...
Characterization of novel cofactorless self-cleaving RNAs with activity at low pH [0.03%]
具有低pH活性的新型无辅因子自切割RNA的特征研究
Nae Sakimoto,Emily Matsusaka,Natsuki Doi et al.
Nae Sakimoto et al.
Several diseases abnormally lower cellular pH owing to metabolic pathway changes. Developing drugs based on genetic material that act only in target tissues may reduce side effects by exploiting the differences between diseased and normal t...
Ant abaecin-2 is a context-dependent copper-binding effector that can be either inhibitory or protective [0.03%]
abaecin-2抗菌肽是铜依赖性结合蛋白，可以抑制也可保护真菌感染肠道的宿主健康
Caroline M Donaghy,Helena V Heyer-Gray,Charlotte O&#x;Hern et al.
Caroline M Donaghy et al.
Host defense peptides (HDPs) are important components of the innate immune system that are used to combat pathogens and often rely on binding trace nutrient metals for their function. However, controlling nutrient metals may have other role...
Yi Yang,Takayoshi Awakawa,Ikuro Abe
Yi Yang
Orthoesters are unique functional groups consisting of three alkoxy substituents attached to a central carbon atom, which endow orthoester-bearing compounds with structural complexity and therapeutically valuable bioactivity. As orthoester ...
Dissecting the functional divergence of DCAF11 isoforms in protein degradation [0.03%]
分析DCAF11不同亚型在蛋白质降解中的功能差异
Xiaokang Jin,Xiaoyu Zhang
Xiaokang Jin
DCAF11 is a substrate receptor of the Cullin-RING ligase 4 (CRL4) ubiquitin ligase complex and an emerging effector supporting targeted protein degradation. DCAF11 exists as two major isoforms, but their functional differences remain incomp...
Jacob Byerly-Duke,Sarah M Bernhard,Rida Ibrahim et al.
Jacob Byerly-Duke et al.
The therapeutic potential of peptides is severely limited by rapid metabolism mediated by proteases. Traditional stabilization strategies often compromise pharmacological profiles by disrupting native conformation or physicochemical propert...
Advances in monitoring mammalian circadian components and their rhythms using reporter systems [0.03%]
运用报告系统监测哺乳动物昼夜成分及其节律的进展
Bhavna Kalyanaraman,Emmanuel F Rivera-Iglesias,Michelle E Farkas
Bhavna Kalyanaraman
Circadian rhythms are innate biological processes that cycle over periods of approximately 24 hours and are crucial in regulating many physiological and metabolic functions. At the molecular level in mammals, these cell-autonomous oscillati...
Exclusion of RNA-binding domains from G-quadruplex condensates by G-quadruplex ligands [0.03%]
G-四链体结合配体使RNA结合域从G-四链体凝集物中解离出去
Yoshiki Hashimoto,Ryosuke Suzuki,Mizuho Aya et al.
Yoshiki Hashimoto et al.
Biomolecular liquid-liquid phase separation, which forms droplets in living cells, plays a crucial role in the regulation of gene expression. Dysfunction of liquid-liquid phase separation leads to aberrant aggregates that sequester RNA-bind...