Crystal structure reveals the hydrophilic R1 group impairs NDM-1-ligand binding via water penetration at L3 [0.03%]
晶体结构显示，亲水性R1基团通过L3处的水分子渗入损害NDM-1配体结合能力
Xiangrui Shi,Huijuan Yang,Yujie Dai et al.
Xiangrui Shi et al.
The global spread of New Delhi metallo-β-lactamases (NDMs) has exacerbated the antimicrobial resistance crisis. This study resolved the crystal structure of NDM-1 hydrolyzing amoxicillin for the first time, revealed that the hydroxyl group...
Indel-driven evolution of the canavanine tRNA-editing deacetylase enzyme CtdA [0.03%]
由插入缺失驱动的_canavanine_tRN_编辑脱乙酰酶酶_CtdA_的进化
Nino Tabagari,Franziskus Hauth,Jennifer R Fleming et al.
Nino Tabagari et al.
Proteins are heteropolymers composed of twenty standard amino acids, but over 500 non-proteogenic amino acids exist in nature that can be misincorporated into proteins. Canavanine is an antimetabolite of the chemically similar L-arginine. I...
Xiao Wang,Yuan-Yuan Li,Zi-Yan Dou et al.
Xiao Wang et al.
The 3'-terminal CCA-end of tRNA is essential for the attachment of amino acids and correct positioning of the aminoacyl-tRNA in the ribosome. In higher plants, the CCA sequence is synthesized, maintained, and repaired by class-II CCA-adding...
Snake venom defensins: Defining the structural and functional characteristics of the toxin family [0.03%]
蛇毒(defensin): 定义毒素家族的结构和功能特征
David Melendez-Martinez,Adriana Morales-Martinez,Iliana Vanessa Almanza-Campos et al.
David Melendez-Martinez et al.
Snake venom defensins are a toxin family found in rattlesnake venoms (Crotalus) which are comprised of crotamine-like peptides and myotoxins. Their tertiary structure resembles the β-defensin family structure. Toxins from this family, such...
Tracing the aggregation pathway of the scaffold protein DISC1: Structural implications for chronic mental illnesses [0.03%]
精神疾病发病的结构基础：DISC1蛋白聚集机理研究
Abhishek Cukkemane,Nina Becker,Tatsiana Kupreichyk et al.
Abhishek Cukkemane et al.
Disrupted in schizophrenia 1 (DISC1) is a pleiotropic scaffold protein that is postulated to comprise large disordered regions and four distinct structured segments with a high proportion of helical or coiled-coil fold. DISC1 associates wit...
pytom-match-pick: A tophat-transform constraint for automated classification in template matching [0.03%]
pytom-match-pick：模板匹配中的自动分类的 tophat-transform 约束条件
Marten L Chaillet,Sander Roet,Remco C Veltkamp et al.
Marten L Chaillet et al.
Template matching (TM) in cryo-electron tomography (cryo-ET) enables in situ detection and localization of known macromolecules. However, TM faces challenges of weak signal of the macromolecules and interfering features with a high signal-t...
Generation of shark single-domain antibodies as an aid for Cryo-EM structure determination of membrane proteins: Use hyaluronan synthase as an example [0.03%]
生成鲨鱼单结构域抗体以辅助膜蛋白冷冻EM结构测定——以透明质酸合成酶为例
Penghui Deng,Xiaoyue Zhang,Jianqing Wen et al.
Penghui Deng et al.
In cartilaginous fish, the immunoglobulin new antigen receptor (IgNAR) is naturally devoid of light chains. The variable regions of IgNAR (VNARs) are solely responsible for antigen recognition, similar to VHHs (variable domain of the heavy ...
Localization of albumin with correlative super resolution light- and electron microscopy in the kidney [0.03%]
超分辨率光学显微镜和电子显微镜关联成像在肾脏定位白蛋白
Alexandra N Birtasu,Utz H Ermel,Johanna V Rahm et al.
Alexandra N Birtasu et al.
The functioning of vertebrate life relies on renal filtration of surplus fluid and elimination of low-molecular-weight waste products, while keeping serum proteins in the blood. In disease, however, there is leak of serum proteins and traci...
Cryo-EM structure of a phosphotransferase system glucose transporter stalled in an intermediate conformation [0.03%]
处于中间构象的停滞的磷酸转移系统葡萄糖转运蛋白的冷冻电镜结构
Patrick Roth,Dimitrios Fotiadis
Patrick Roth
The phosphotransferase system glucose-specific transporter IICBGlc serves as a central nutrient uptake system in bacteria. It transports glucose across the plasma membrane via the IICGlc domain and phosphorylates the substrate within the ce...
Molecular biophysics and inhibition mechanism of influenza virus A M2 viroporin by adamantane-based drugs - Challenges in designing antiviral agents [0.03%]
分子生物物理学及基于金刚烷的药物对甲型流感病毒M2病毒孔蛋白的抑制机制-设计抗病毒剂所面临的挑战
Kyriakos Georgiou,Dimitrios Kolokouris,Antonios Kolocouris
Kyriakos Georgiou
The influenza A matrix 2 (AM2) protein is a prototype viroporin that conducts protons through an array of water molecules and sidechains of ionizable amino acid residues, with His37 being the most important. Amantadine is a prototype AM2 ch...