Structural insights of the coronavirus main protease in complex with the non-covalent inhibitor CCF0058981 [0.03%]
非共价抑制剂CCF0058981与冠状病毒主要蛋白酶复合物的结构洞察
Pei Zeng,Xuelan Zhou,Li Guo et al.
Pei Zeng et al.
The highly pathogenic SARS-CoV-2 causes COVID-19, which threatens global public health and socio-economic stability through persistent transmission and mutation. Effective therapeutics against SARS-CoV-2 and its variants are urgently needed...
Ruizhi Peng,Heidy Elkhaligy,Timothy Grant et al.
Ruizhi Peng et al.
Tubular structures are ubiquitous in biological systems and have been a focal point of cryo-electron microscopy (cryo-EM) structural analysis since the technique's inception. A critical step in processing tubular cryo-EM data is particle cl...
The structure of the bacterial outer membrane transporter FusA enabled by addition of the native lipid lipopolysaccharide [0.03%]
天然脂质脂多糖的添加促成的细菌外膜转运蛋白FusA的结构
Jonathan M Machin,Khedidja Mosbahi,Dheeraj Prakaash et al.
Jonathan M Machin et al.
Lipopolysaccharide (LPS) is a glycolipid found uniquely in the outer membrane of diderm bacteria, formed of 4-7 acyl chains covalently linked to an extended polysaccharide chain. While a few examples of the interaction between LPS and outer...
Calcium stabilizes the flexible N-terminal domain of the bacterial ion channel DeCLIC [0.03%]
钙稳定细菌离子通道DeClic的柔性N端结构域
Chen Fan,Marie Lycksell,Yuxuan Zhuang et al.
Chen Fan et al.
Pentameric ligand-gated ion channels (pLGICs) are responsible for the rapid conversion of chemical to electrical signals. In addition to the canonical extracellular and transmembrane domains, some prokaryotic pLGICs contain an N-terminal do...
Hemoglobin receptor redundancy in Staphylococcus aureus: molecular flexibility as a determinant of divergent hemophore activity [0.03%]
金黄色葡萄球菌血红蛋白受体的冗余性及其分子灵活性决定的不同血红素载体的作用机制
Valeria Buoli Comani,Omar De Bei,Francesca Pancrazi et al.
Valeria Buoli Comani et al.
To overcome iron limitation in the host, Staphylococcus aureus exploits sophisticated mechanisms to acquire this essential nutrient, particularly from hemoglobin (Hb). The bacterial hemophores IsdH and IsdB play key roles in binding Hb and ...
Structural characterization and inhibition of carbonic anhydrase from Candida parapsilosis [0.03%]
粗球壳酵母碳酸酐酶的结构分析及抑制研究
Jiří Dostál,Zdeňka Uhrová,Magdalena Škrlová et al.
Jiří Dostál et al.
Fungal carbonic anhydrases (CAs) are metalloenzymes that catalyze the reversible hydration of carbon dioxide and play an essential role in fungal adaptation to environments with fluctuating CO2 concentrations. The opportunistic pathogen Can...
Residues of the ribose binding site are required for human ribokinase activity [0.03%]
核糖结合位点遗迹残基对于人核糖激酶活性是必需的
Juliana C Ferreira,Lyudmila Nedyalkova,Adrian J Villanueva et al.
Juliana C Ferreira et al.
Ribokinase (RK) catalyzes the phosphorylation of D-ribose to ribose-5-phosphate, an essential metabolic intermediate for the pentose phosphate pathway, nucleotide biosynthesis, redox balance, and cellular energy metabolism. Despite its cent...
The J-shape of β2GPI reveals a cryptic discontinuous epitope across domains I and II [0.03%]
β2GP1的J形构象揭示了I和II结构域之间的隐形不连续表位
C J Lalaurie,M Kulke,N Geist et al.
C J Lalaurie et al.
Beta-2-Glycoprotein I is the main target for pathogenic antiphospholipid syndrome autoantibodies. It can adopt several conformations, including an O-shape and two more linear J- and S-shapes. The in vivo existence of the O-shape is debated,...
Structural analysis of TRIM family PRYSPRY domains and its implications for E3-ligand design [0.03%]
TRIM家族PRYSPRY结构域的结构分析及其对E3泛素连接酶设计的启示
Rezart Zhubi,Apirat Chaikuad,Christian J Muñoz Sosa et al.
Rezart Zhubi et al.
Tripartite motif (TRIM) proteins constitute one of the largest subfamilies of RING-type E3 ubiquitin ligases and are attractive targets for the development of novel degraders that exploit the ubiquitin-proteasome pathway. More than half of ...
Tomáš Majtner,Beata Turoňová
Tomáš Majtner
Cryo-electron tomography (cryo-ET) enables the visualization of macromolecular structures in their near-native cellular environment. However, acquired tilt series are often compromised by image corruption due to drift, contamination, and ic...