Trans-interface coupling in the tetrameric enzyme underlies allosteric control in Escherichia coli biosynthetic threonine deaminase [0.03%]
四聚体酶的跨界面耦合是大肠杆菌合成型丝氨酸脱氨酶别构调控的基础
Samaneh Khodi,Abeeb Abiodun Yekeen,Haiyan Liu et al.
Samaneh Khodi et al.
Allosteric regulation in biosynthetic L-threonine deaminase (BTD) has long been attributed to effector-induced conformational changes. Here, we identify a trans-interface coupling mechanism that regulates the active site architecture of EcI...
Structural and functional characterisation of the dextran utilisome from Bacteroides thetaiotaomicron [0.03%]
d-半乳糖醛酸聚合物利用蛋白体的结构与功能鉴定：以拟杆菌门.thetaoinc.为例
Matthew Feasey,Augustinas Silale,Arnaud Baslé et al.
Matthew Feasey et al.
Bacteroides thetaiotaomicron (B. theta) is a model Bacteroidota of the healthy human gut microbiota and a specialist in glycan utilisation. Like other Bacteroides, B. theta has many highly regulated polysaccharide utilisation loci (PUL) tha...
J Wouter Beugelink,Bert J C Janssen
J Wouter Beugelink
Members of the Vps10p receptor family regulate protein trafficking and cellular differentiation in the nervous system. Previous structural studies of the dimeric Vps10p family member SorCS2 have focused on isolated ectodomains, revealing su...
Structural and chemical heterogeneity of pulp stones in young untreated bovine incisors: frequent dentin-like mineralizations [0.03%]
年轻未治疗的牛门牙中牙本质样矿化的牙石的结构和化学异质性
Hamza Elfarraj,Oleksandra Marushchenko,Franco Lizzi et al.
Hamza Elfarraj et al.
Pulp stone research has predominantly relied on aged or clinically treated human teeth, limiting insights into early-stage mineralization processes. This study investigates young bovine incisors (3-4 years old, ≤2 years after eruption) as ...
Bipixel rotation method: An algorithm for chirality analysis of biological mesh network [0.03%]
生物网状网络手性分析的双像素旋转法
Kanta Kumaki,Katsuhiro Kawaai,Shinobu Noji et al.
Kanta Kumaki et al.
Vertebrates are bilateral organisms characterized by both symmetrical and asymmetrical structures. While skeletons are grossly symmetrical, microscopic biological networks within the bone-such as cortical canals containing vascular networks...
Alanine scanning of the yeast killer toxin K2 reveals key residues for activity, gain-of-function variants, and supports prediction of precursor processing and 3D structure [0.03%]
丙氨酸扫描筛选酵母杀手毒素K2的活性关键位点，并发现功能获得性变异体，且可验证前体蛋白加工及三维结构预测
Rianne C Prins,Tycho Marinus,Eyal Dafni et al.
Rianne C Prins et al.
Yeast killer toxins (YKTs) are antimicrobial proteins secreted by yeast with potential applications ranging from food preservation to therapeutic agents in human health. However, the practical use of many YKTs is limited by specific pH requ...
High resolution cryo-EM structure of the Pseudomonas aeruginosa OprM multidrug efflux pump provides target for rational inhibitor design [0.03%]
高分辨率的铜绿假单胞菌外排泵OprM冷冻电镜结构为理性抑制剂设计提供靶标
Anna C Ratliff,Istvan Botos,Amanda M Putti et al.
Anna C Ratliff et al.
OprM is the outer membrane channel component of resistance-nodulation-division (RND) efflux pumps in Pseudomonas aeruginosa, a high-priority human pathogen. OprM-dependent pumps play diverse roles in bacterial physiology and mediate resista...
Structural characterization of an α-glucosaccharide-binding protein from Paenibacillus sp. str. FPU-7 [0.03%]
来自Paenibacillus sp. str. FPU-7的α-葡萄糖结合蛋白的结构表征
Takafumi Itoh,Kanato Kataoka,Yuma Kaneko et al.
Takafumi Itoh et al.
Paenibacillus sp. str. FPU-7 (P. FPU-7), a chitinolytic bacterium, efficiently degrades chitin and uses the solute-binding proteins (SBPs) NagB1 and NagB2 on the cell surface to facilitate the uptake and intracellular transport of chitoolig...
Thraustochytrid PUFA synthase ER domains form a stable heterodimer [0.03%]
硫藻游仆菌PUFA合成酶内质网结构域形成稳定的异二聚体
Nahuel Lofeudo,Gabriel Moncalian
Nahuel Lofeudo
Omega-3 polyunsaturated fatty acids (PUFAs) are essential nutrients for humans and are synthesized de novo by specialized enzymes known as PUFA synthases (Pfas). The domains of these enzymes are structurally related to those of mammalian or...
Inhibitory effects and structural analysis of drug candidate PF-07817883 against coronaviral main proteases (Mpro) and SARS-CoV-2 Mpro mutants [0.03%]
PF-07817883抑制冠状病毒主要蛋白酶（Mpro）和SARS-CoV-2 Mpro突变体的活性及结构分析
Zhenyu Zhao,Xuelan Zhou,Haihai Jiang et al.
Zhenyu Zhao et al.
Main proteases (Mpros) are a class of conserved cysteine proteases found among coronaviruses, playing a key role in their replication. These properties make Mpros ideal targets for the screening and design of pan-coronavirus drugs. Recently...